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ITPKA (NM_002220) Human Tagged ORF Clone
CAT＃: RC205323
ITPKA (Myc-DDK-tagged)-Human inositol-trisphosphate 3-kinase A (ITPKA)
ORF Plasmid: tGFP
"NM_002220" in other vectors (4)
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CNY 3,656.00
CNY 4,090.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | IP3-3KA; IP3KA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205323 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCTGCCCGGGGGCCCAACGGGCATGGCGCGGCCGGGGGGCGCGAGGCCCTGCAGCCCGGGGCTGG AGCGGGCCCCGCGCAGGAGTGTCGGGGAGCTGCGCCTGCTCTTCGAGGCGCGCTGTGCGGCGGTCGCTGC GGCCGCCGCCGCGGGGGAGCCCCGGGCCCGCGGGGCCAAGCGGCGTGGGGGACAGGTCCCCAACGGGCTT CAGCGGGCTCCCCCGGCCCCGGTGATCCCTCAGCTGACCGTGACAGCCGAGGAGCCCGACGTGCCCCCGA CCAGCCCTGGGCCGCCGGAGCGGGAGAGGGACTGCCTCCCGGCAGCGGGCTCTTCGCACCTGCAGCAGCC GCGCCGCCTTTCCACCTCGTCGGTCTCCTCCACTGGCTCCTCGTCGCTGCTCGAGGACTCGGAGGACGAC CTGCTGAGCGACAGTGAGAGCCGGAGCCGCGGCAACGTGCAGCTGGAAGCGGGCGAGGACGTGGGTCAGA AAAACCACTGGCAGAAGATCCGGACCATGGTCAATCTGCCGGTCATAAGCCCTTTCAAGAAGCGCTACGC CTGGGTGCAGCTGGCAGGGCACACTGGGAGTTTTAAGGCGGCGGGCACCAGCGGGCTGATCCTGAAGCGC TGCTCGGAGCCGGAGCGCTACTGCCTGGCGCGGCTGATGGCTGACGCGCTGCGCGGCTGCGTGCCTGCCT TCCACGGCGTGGTGGAGCGCGACGGCGAAAGCTACCTGCAGCTGCAGGACCTGCTCGATGGCTTCGACGG ACCTTGTGTGCTCGACTGCAAAATGGGCGTCAGGACTTACCTAGAGGAGGAGCTGACCAAGGCCCGTGAG CGGCCCAAGCTGCGGAAGGACATGTACAAGAAAATGCTGGCGGTGGATCCTGAAGCTCCCACGGAGGAGG AGCACGCGCAGCGCGCCGTCACCAAGCCGCGCTACATGCAGTGGCGGGAAGGCATCAGCTCCAGCACCAC CCTCGGCTTCCGCATCGAGGGCATCAAGAAAGCGGACGGCTCCTGCAGCACCGACTTCAAGACTACGCGA AGCCGAGAGCAGGTGCTTCGCGTCTTTGAAGAGTTTGTGCAAGGAGATGAGGAAGTGCTGAGGCGGTATC TGAACCGCCTGCAGCAGATCCGGGACACCCTGGAGGTATCCGAGTTCTTCAGGAGGCACGAGGTGATCGG CAGCTCGCTCCTCTTTGTGCACGATCACTGCCATCGCGCCGGCGTGTGGCTCATCGACTTCGGCAAGACC ACGCCCCTCCCCGATGGCCAGATCCTGGACCACCGGCGGCCCTGGGAGGAGGGCAACCGCGAGGACGGCT ATTTGCTGGGGCTGGACAATCTCATTGGCATCCTGGCCAGCCTGGCTGAGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205323 protein sequence
Red=Cloning site Green=Tags(s) MTLPGGPTGMARPGGARPCSPGLERAPRRSVGELRLLFEARCAAVAAAAAAGEPRARGAKRRGGQVPNGL QRAPPAPVIPQLTVTAEEPDVPPTSPGPPERERDCLPAAGSSHLQQPRRLSTSSVSSTGSSSLLEDSEDD LLSDSESRSRGNVQLEAGEDVGQKNHWQKIRTMVNLPVISPFKKRYAWVQLAGHTGSFKAAGTSGLILKR CSEPERYCLARLMADALRGCVPAFHGVVERDGESYLQLQDLLDGFDGPCVLDCKMGVRTYLEEELTKARE RPKLRKDMYKKMLAVDPEAPTEEEHAQRAVTKPRYMQWREGISSSTTLGFRIEGIKKADGSCSTDFKTTR SREQVLRVFEEFVQGDEEVLRRYLNRLQQIRDTLEVSEFFRRHEVIGSSLLFVHDHCHRAGVWLIDFGKT TPLPDGQILDHRRPWEEGNREDGYLLGLDNLIGILASLAER TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002220 |
| ORF Size | 1383 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002220.3 |
| RefSeq Size | 1864 bp |
| RefSeq ORF | 1386 bp |
| Locus ID | 3706 |
| UniProt ID | P23677 |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| MW | 51 kDa |
| Gene Summary | Regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of the inositol 1,4,5-trisphosphate 3-kinase is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. It is also a substrate for the cyclic AMP-dependent protein kinase, calcium/calmodulin- dependent protein kinase II, and protein kinase C in vitro.[provided by RefSeq, Apr 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205323L3 | Lenti ORF clone of Human inositol-trisphosphate 3-kinase A (ITPKA), Myc-DDK-tagged |
CNY 5,990.00 |
|
| RC205323L4 | Lenti ORF clone of Human inositol-trisphosphate 3-kinase A (ITPKA), mGFP tagged |
CNY 5,990.00 |
|
| RG205323 | ITPKA (tGFP-tagged) - Human inositol-trisphosphate 3-kinase A (ITPKA) |
CNY 4,470.00 |
|
| SC303150 | ITPKA (untagged)-Human inositol-trisphosphate 3-kinase A (ITPKA) |
CNY 3,656.00 |
