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ACADSB (NM_001609) Human Tagged ORF Clone
CAT＃: RC204465
ACADSB (Myc-DDK-tagged)-Human acyl-Coenzyme A dehydrogenase, short/branched chain (ACADSB), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_001609" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2-MEBCAD; ACAD7; SBCAD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204465 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGCCTGGCAGTGCGGTTGCTGCGCGGCAGCAAGCTGCTAAGAAGAAATTTCCTGACTTGTTTGT CTTCTTGGAAGATTCCTCCTCATGTCTCAAAATCTTCCCAGTCAGAAGCTCTACTCAATATAACAAATAA TGGAATACACTTTGCTCCCCTGCAAACATTTACAGATGAGGAAATGATGATAAAGAGTTCAGTTAAAAAA TTTGCTCAGGAACAAATTGCACCTTTGGTTTCAACCATGGATGAAAATTCGAAAATGGAGAAATCAGTAA TACAAGGATTATTTCAACAAGGGTTGATGGGTATTGAAGTTGACCCAGAATATGGAGGCACAGGAGCTTC ATTTTTATCCACTGTGCTCGTGATAGAGGAATTAGCCAAAGTTGATGCATCTGTGGCTGTCTTTTGTGAG ATCCAGAACACATTAATTAACACACTGATTAGAAAACATGGAACAGAAGAACAAAAGGCCACCTATTTGC CTCAGCTCACTACAGAAAAAGTAGGAAGTTTCTGCCTTTCAGAGGCTGGAGCAGGTAGTGACTCATTTGC TTTGAAGACCAGAGCTGATAAAGAGGGAGATTATTATGTCCTCAATGGATCAAAGATGTGGATCAGCAGT GCTGAGCACGCAGGGCTCTTTCTGGTGATGGCAAATGTAGACCCTACCATTGGATATAAGGGAATTACCT CCTTCTTAGTAGATCGTGATACTCCGGGCCTTCATATAGGGAAACCTGAAAACAAATTGGGGCTCAGAGC TTCTTCCACCTGCCCGTTAACATTCGAAAATGTCAAGGTTCCAGAAGCCAATATCTTGGGACAAATTGGA CATGGCTATAAGTATGCCATAGGGAGTCTCAATGAAGGTAGAATAGGAATTGCTGCACAGATGCTGGGAC TGGCGCAAGGATGTTTTGACTACACTATTCCATATATTAAAGAAAGGATACAATTTGGCAAAAGACTATT TGATTTTCAGGGCCTCCAACACCAAGTGGCTCACGTGGCCACCCAGCTGGAAGCTGCAAGATTACTAACA TACAATGCTGCTAGGCTTTTAGAAGCTGGAAAGCCATTCATAAAAGAAGCGTCAATGGCCAAATACTATG CATCAGAGATTGCAGGACAAACAACGAGTAAATGTATCGAGTGGATGGGGGGAGTAGGCTACACCAAAGA TTACCCTGTGGAGAAATACTTCCGAGATGCAAAGATTGGTACGATATATGAAGGAGCTTCCAACATCCAG TTGAACACCATTGCAAAGCATATCGATGCAGAATAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204465 protein sequence
Red=Cloning site Green=Tags(s) MEGLAVRLLRGSKLLRRNFLTCLSSWKIPPHVSKSSQSEALLNITNNGIHFAPLQTFTDEEMMIKSSVKK FAQEQIAPLVSTMDENSKMEKSVIQGLFQQGLMGIEVDPEYGGTGASFLSTVLVIEELAKVDASVAVFCE IQNTLINTLIRKHGTEEQKATYLPQLTTEKVGSFCLSEAGAGSDSFALKTRADKEGDYYVLNGSKMWISS AEHAGLFLVMANVDPTIGYKGITSFLVDRDTPGLHIGKPENKLGLRASSTCPLTFENVKVPEANILGQIG HGYKYAIGSLNEGRIGIAAQMLGLAQGCFDYTIPYIKERIQFGKRLFDFQGLQHQVAHVATQLEAARLLT YNAARLLEAGKPFIKEASMAKYYASEIAGQTTSKCIEWMGGVGYTKDYPVEKYFRDAKIGTIYEGASNIQ LNTIAKHIDAEY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001609 |
| ORF Size | 1296 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001609.4 |
| RefSeq Size | 5941 bp |
| RefSeq ORF | 1299 bp |
| Locus ID | 36 |
| UniProt ID | P45954 |
| Domains | Acyl-CoA_dh, Acyl-CoA_dh_M, Acyl-CoA_dh_N |
| Protein Pathways | Fatty acid metabolism, Metabolic pathways, Valine, leucine and isoleucine degradation |
| MW | 47.5 kDa |
| Gene Summary | Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204465L3 | Lenti ORF clone of Human acyl-Coenzyme A dehydrogenase, short/branched chain (ACADSB), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204465L4 | Lenti ORF clone of Human acyl-Coenzyme A dehydrogenase, short/branched chain (ACADSB), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RG204465 | ACADSB (tGFP-tagged) - Human acyl-Coenzyme A dehydrogenase, short/branched chain (ACADSB), nuclear gene encoding mitochondrial protein |
CNY 4,370.00 |
|
| SC321668 | ACADSB (untagged)-Human acyl-Coenzyme A dehydrogenase, short/branched chain (ACADSB), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
