CCDC34 (NM_080654) Human Tagged ORF Clone

CAT#: RC203345

CCDC34 (Myc-DDK-tagged)-Human coiled-coil domain containing 34 (CCDC34), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_080654" in other vectors (5)

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CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-CCDC34 Antibody
    • 50 ug

CNY 5,200.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms L15; NY-REN-41; RAMA3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203345 representing NM_080654
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGGCGGCGGGGCGCTGGGGGCCTACTTTTCCCTCTTCCTACGCCGGTTTCTCTGCTGACTGCAGAC
CCAGGTCTCGGCCCTCCTCGGACTCCTGCTCAGTCCCTATGACGGGCGCACGTGGGCAGGGGCTGGAGGT
GGTGCGCTCGCCGTCGCCGCCGCTGCCGCTGAGCTGCAGCAATTCCACCAGGTCGCTGTTGTCTCCCCTT
GGCCACCAGAGCTTCCAGTTTGACGAGGACGACGGTGACGGGGAGGATGAGGAAGACGTGGATGATGAGG
AAGACGTGGATGAAGATGCCCATGATTCAGAGGCCAAAGTGGCGAGCCTGAGAGGAATGGAGTTACAGGG
GTGCGCCAGCACTCAGGTTGAATCAGAAAATAACCAAGAAGAACAGAAACAGGTGCGCTTACCAGAAAGC
CGCCTGACACCATGGGAGGTGTGGTTTATTGGCAAAGAAAAAGAAGAACGTGACCGGCTGCAACTGAAAG
CTCTAGAGGAATTAAATCAACAACTAGAAAAAAGAAAAGAAATGGAAGAACGTGAAAAAAGAAAGATAAT
TGCTGAAGAAAAGCACAAGGAATGGGTTCAGAAAAAGAATGAGCAAGTAAGGAGGGGAAAATGGATACAC
ACATTGACATCTCTTTTGCAAAATATTTCTTCCTATTATACTTCATTACCTAGGTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203345 representing NM_080654
Red=Cloning site Green=Tags(s)

MWAAGRWGPTFPSSYAGFSADCRPRSRPSSDSCSVPMTGARGQGLEVVRSPSPPLPLSCSNSTRSLLSPL
GHQSFQFDEDDGDGEDEEDVDDEEDVDEDAHDSEAKVASLRGMELQGCASTQVESENNQEEQKQVRLPES
RLTPWEVWFIGKEKEERDRLQLKALEELNQQLEKRKEMEEREKRKIIAEEKHKEWVQKKNEQVRRGKWIH
TLTSLLQNISSYYTSLPRF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_080654
ORF Size 687 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_080654.2, NP_542385.1
RefSeq Size 2120 bp
RefSeq ORF 690 bp
Locus ID 91057
UniProt ID Q96HJ3
MW 26.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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