PPP6C (NM_002721) Human Tagged ORF Clone

CAT#: RC202684

  • TrueORF®

PPP6C (Myc-DDK-tagged)-Human protein phosphatase 6, catalytic subunit (PPP6C), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_002721" in other vectors (7)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit anti-PPP6C polyclonal antibody
    • 100 ul

CNY 3,330.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms PP6; PP6C
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202684 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCCGCTAGACCTGGACAAGTATGTGGAAATAGCGCGGCTGTGCAAGTACCTGCCAGAGAACGACC
TGAAGCGGCTATGTGACTACGTTTGTGACCTCCTCTTAGAAGAGTCAAATGTTCAGCCAGTATCAACACC
AGTAACAGTGTGTGGAGATATCCATGGACAGTTTTATGACCTTTGTGAACTGTTCAGAACTGGAGGTCAG
GTTCCTGACACAAACTACATATTTATGGGTGATTTTGTAGACAGAGGTTACTATAGTTTGGAGACCTTCA
CTTACCTTCTTGCATTAAAGGCTAAATGGCCTGATCGTATTACACTTTTGCGAGGAAATCATGAGAGTAG
ACAGATAACACAGGTCTATGGATTTTATGATGAGTGCCAAACCAAATATGGAAATGCTAATGCCTGGAGA
TACTGTACCAAAGTTTTTGACATGCTCACAGTAGCAGCTTTAATAGATGAGCAGATTTTGTGTGTCCATG
GTGGTTTATCTCCTGATATCAAAACACTGGATCAAATTCGAACCATCGAACGGAATCAGGAAATTCCTCA
TAAAGGAGCATTTTGTGATCTGGTTTGGTCAGATCCTGAAGATGTGGATACCTGGGCTATCAGTCCCCGA
GGAGCAGGTTGGCTTTTTGGAGCAAAGGTCACAAATGAGTTTGTTCATATCAACAACTTAAAACTCATCT
GCAGAGCACATCAACTAGTGCACGAAGGCTATAAATTTATGTTTGATGAGAAGCTGGTGACAGTATGGTC
TGCTCCTAATTACTGCTATCGTTGTGGAAATATTGCTTCGATCATGGTCTTCAAAGATGTAAATACAAGA
GAACCAAAGTTATTCCGGGCAGTTCCAGATTCAGAACGTGTTATTCCTCCCAGAACGACAACGCCATATT
TCCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202684 protein sequence
Red=Cloning site Green=Tags(s)

MAPLDLDKYVEIARLCKYLPENDLKRLCDYVCDLLLEESNVQPVSTPVTVCGDIHGQFYDLCELFRTGGQ
VPDTNYIFMGDFVDRGYYSLETFTYLLALKAKWPDRITLLRGNHESRQITQVYGFYDECQTKYGNANAWR
YCTKVFDMLTVAALIDEQILCVHGGLSPDIKTLDQIRTIERNQEIPHKGAFCDLVWSDPEDVDTWAISPR
GAGWLFGAKVTNEFVHINNLKLICRAHQLVHEGYKFMFDEKLVTVWSAPNYCYRCGNIASIMVFKDVNTR
EPKLFRAVPDSERVIPPRTTTPYFL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002721
ORF Size 915 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002721.5
RefSeq Size 4256 bp
RefSeq ORF 918 bp
Locus ID 5537
UniProt ID O00743
Domains Metallophos, PP2Ac
Protein Families Druggable Genome, Phosphatase
MW 35.1 kDa
Gene Summary This gene encodes the catalytic subunit of protein phosphatase, a component of a signaling pathway regulating cell cycle progression. Splice variants encoding different protein isoforms exist. The pseudogene of this gene is located on chromosome X. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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