UBE2H (NM_003344) Human Tagged ORF Clone
CAT#: RC202516
UBE2H (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 1
ORF Plasmid: tGFP
"NM_003344" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | E2-20K; GID3; UBC8; UBCH; UBCH2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202516 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATCTCCCAGTCCGGGCAAGAGGCGGATGGACACGGACGTGGTCAAGCTCATCGAGAGTAAACATG AGGTTACGATCCTGGGAGGACTTAATGAATTTGTAGTGAAGTTTTATGGACCACAAGGAACACCATATGA AGGCGGAGTATGGAAAGTTAGAGTGGACCTACCTGATAAATACCCTTTCAAATCTCCATCTATAGGATTC ATGAATAAAATTTTCCATCCCAACATTGATGAAGCGTCAGGAACTGTGTGTCTAGATGTAATTAATCAAA CTTGGACAGCTCTCTATGATCTTACCAATATATTTGAGTCCTTCCTGCCTCAGTTATTGGCCTATCCTAA CCCCATAGATCCTCTCAATGGTGACGCTGCAGCCATGTACCTCCACCGACCAGAAGAATACAAGCAGAAA ATTAAAGAGTACATCCAGAAATACGCCACGGAGGAGGCGCTGAAAGAACAGGAAGAGGGTACCGGGGACA GCTCATCGGAGAGCTCTATGTCTGACTTTTCCGAAGATGAGGCCCAGGATATGGAGTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202516 protein sequence
Red=Cloning site Green=Tags(s) MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIGF MNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQK IKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_003344 |
ORF Size | 549 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003344.4 |
RefSeq Size | 5174 bp |
RefSeq ORF | 552 bp |
Locus ID | 7328 |
UniProt ID | P62256 |
Domains | UBCc |
Protein Pathways | Ubiquitin mediated proteolysis |
MW | 20.7 kDa |
Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation
,Li, K;Wang, F;Yang, ZN;Zhang, TT;Yuan, YF;Zhao, CX;Yeerjiang, Z;Cui, B;Hua, F;Lv, XX;Zhang, XW;Yu, JJ;Liu, SS;Yu, JM;Shang, S;Xiao, Y;Hu, ZW;,
Nature communications
,PubMed ID 33298911
[UBE2H]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202516L1 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC202516L2 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC202516L3 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC202516L4 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG202516 | UBE2H (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 1 |
CNY 4,000.00 |
|
SC111701 | UBE2H (untagged)-Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 1 |
CNY 2,400.00 |