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ILKAP (NM_030768) Human Tagged ORF Clone
CAT＃: RC201456
ILKAP (Myc-DDK-tagged)-Human integrin-linked kinase-associated serine/threonine phosphatase (ILKAP)
ORF Plasmid: tGFP
"NM_030768" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ILKAP2; ILKAP3; PP2C-DELTA; PP2CD; PPM1O |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201456 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTCTTCGGGGACCTGCCGGAGCCCGAGCGCTCGCCGCGCCCGGCTGCCGGGAAAGAAGCTCAGA AAGGACCCCTGCTCTTTGATGACCTCCCTCCGGCCAGCAGTACTGACTCAGGATCAGGGGGACCTTTGCT TTTTGATGATCTCCCACCCGCTAGCAGTGGCGATTCAGGTTCTCTTGCCACATCAATATCCCAGATGGTA AAGACTGAAGGGAAAGGAGCAAAGAGAAAAACCTCCGAGGAAGAGAAGAATGGCAGTGAAGAGCTTGTGG AAAAGAAAGTTTGTAAAGCCTCTTCGGTGATCTTTGGTCTGAAGGGCTATGTGGCTGAGCGGAAGGGTGA GAGGGAGGAGATGCAGGATGCCCACGTCATCCTGAACGACATCACCGAGGAGTGTAGGCCCCCATCGTCC CTCATTACTCGGGTTTCATATTTTGCTGTTTTTGATGGACATGGAGGAATTCGAGCCTCAAAATTTGCTG CACAGAATTTGCATCAAAACTTAATCAGAAAATTTCCTAAAGGAGATGTAATCAGTGTAGAGAAAACCGT GAAGAGATGCCTTTTGGACACTTTCAAGCATACTGATGAAGAGTTCCTTAAACAAGCTTCCAGCCAGAAG CCTGCCTGGAAAGATGGGTCCACTGCCACGTGTGTTCTGGCTGTAGACAACATTCTTTATATTGCCAACC TCGGAGATAGTCGGGCAATCTTGTGTCGTTATAATGAGGAGAGTCAAAAACATGCAGCCTTAAGCCTCAG CAAAGAGCATAATCCAACTCAGTATGAAGAGCGGATGAGGATACAGAAGGCTGGAGGAAACGTCAGGGAT GGGCGTGTTTTGGGCGTGCTAGAGGTGTCACGCTCCATTGGGGACGGGCAGTACAAGCGCTGCGGTGTCA CCTCTGTGCCCGACATCAGACGCTGCCAGCTGACCCCCAATGACAGGTTCATTTTGTTGGCCTGTGATGG GCTCTTCAAGGTCTTTACCCCAGAAGAAGCCGTGAACTTCATCTTGTCCTGTCTCGAGGATGAAAAGATC CAGACCCGGGAAGGGAAGTCCGCAGCCGACGCCCGCTACGAAGCAGCCTGCAACAGGCTGGCCAACAAGG CGGTGCAGCGGGGCTCGGCCGACAACGTCACTGTGATGGTGGTGCGGATAGGGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201456 protein sequence
Red=Cloning site Green=Tags(s) MDLFGDLPEPERSPRPAAGKEAQKGPLLFDDLPPASSTDSGSGGPLLFDDLPPASSGDSGSLATSISQMV KTEGKGAKRKTSEEEKNGSEELVEKKVCKASSVIFGLKGYVAERKGEREEMQDAHVILNDITEECRPPSS LITRVSYFAVFDGHGGIRASKFAAQNLHQNLIRKFPKGDVISVEKTVKRCLLDTFKHTDEEFLKQASSQK PAWKDGSTATCVLAVDNILYIANLGDSRAILCRYNEESQKHAALSLSKEHNPTQYEERMRIQKAGGNVRD GRVLGVLEVSRSIGDGQYKRCGVTSVPDIRRCQLTPNDRFILLACDGLFKVFTPEEAVNFILSCLEDEKI QTREGKSAADARYEAACNRLANKAVQRGSADNVTVMVVRIGH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030768 |
| ORF Size | 1176 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030768.3 |
| RefSeq Size | 1480 bp |
| RefSeq ORF | 1179 bp |
| Locus ID | 80895 |
| UniProt ID | Q9H0C8 |
| Domains | PP2C |
| Protein Families | Druggable Genome, Phosphatase |
| MW | 42.9 kDa |
| Gene Summary | The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201456L1 | Lenti ORF clone of Human integrin-linked kinase-associated serine/threonine phosphatase (ILKAP), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201456L2 | Lenti ORF clone of Human integrin-linked kinase-associated serine/threonine phosphatase (ILKAP), mGFP tagged |
CNY 5,890.00 |
|
| RC201456L3 | Lenti ORF clone of Human integrin-linked kinase-associated serine/threonine phosphatase (ILKAP), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201456L4 | Lenti ORF clone of Human integrin-linked kinase-associated serine/threonine phosphatase (ILKAP), mGFP tagged |
CNY 5,890.00 |
|
| RG201456 | ILKAP (tGFP-tagged) - Human integrin-linked kinase-associated serine/threonine phosphatase (ILKAP) |
CNY 4,370.00 |
|
| SC111845 | ILKAP (untagged)-Human integrin-linked kinase-associated serine/threonine phosphatase (ILKAP) |
CNY 2,950.00 |
|
| SC320320 | ILKAP (untagged)-Human integrin-linked kinase-associated serine/threonine phosphatase (ILKAP) |
CNY 2,950.00 |
