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PRPF3 (NM_004698) Human Tagged ORF Clone
CAT＃: RC201365
PRPF3 (Myc-DDK-tagged)-Human PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae) (PRPF3)
ORF Plasmid: tGFP
"NM_004698" in other vectors (4)
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CNY 5,000.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HPRP3; HPRP3P; PRP3; Prp3p; RP18; SNRNP90 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201365 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACTGTCAAAGAGGGAGCTGGATGAGCTGAAACCATGGATAGAGAAGACAGTGAAGAGGGTCCTGG GTTTCTCAGAGCCTACGGTGGTCACAGCAGCATTGAACTGTGTGGGGAAGGGCATGGACAAGAAGAAGGC AGCCGATCATCTGAAACCTTTTCTTGATGATTCTACTCTCCGATTTGTGGACAAACTGTTTGAGGCTGTG GAGGAAGGCCGAAGCTCTAGGCATTCCAAGTCTAGCAGTGACAGGAGCAGAAAACGAGAGCTAAAGGAGG TGTTTGGTGATGACTCTGAGATCTCTAAAGAATCATCAGGAGTAAAGAAGCGACGAATACCCCGTTTTGA GGAGGTGGAAGAAGAGCCAGAGGTGATCCCTGGGCCTCCATCAGAGAGCCCTGGCATGCTGACTAAGCTC CAGATCAAACAGATGATGGAGGCAGCAACACGACAAATCGAGGAGAGGAAAAAACAGCTGAGCTTCATTA GCCCCCCTACACCTCAGCCAAAGACTCCTTCTTCCTCCCAACCAGAACGACTTCCTATTGGCAACACTAT TCAGCCCTCCCAGGCTGCCACTTTCATGAATGATGCCATTGAGAAGGCAAGGAAAGCAGCTGAACTGCAA GCTCGAATCCAAGCCCAGCTGGCACTGAAGCCAGGACTCATCGGCAATGCCAACATGGTGGGCCTGGCTA ATCTCCATGCCATGGGCATTGCTCCCCCGAAGGTGGAGTTAAAAGACCAAACGAAACCTACACCACTGAT CCTGGATGAGCAAGGGCGCACTGTAGATGCAACAGGCAAGGAGATTGAGCTGACACACCGCATGCCTACT CTGAAAGCCAATATTCGTGCTGTGAAGAGGGAACAATTCAAGCAACAACTAAAGGAAAAGCCATCAGAAG ACATGGAATCCAATACCTTTTTTGACCCCCGAGTCTCCATTGCCCCTTCCCAGCGCCAGAGACGCACTTT TAAATTCCATGACAAGGGCAAATTTGAGAAGATTGCTCAGCGATTACGGACAAAGGCTCAACTGGAGAAG CTACAGGCAGAGATTTCACAAGCAGCTCGAAAAACAGGCATCCATACTTCGACTAGGCTTGCCCTCATTG CTCCTAAGAAGGAGCTAAAGGAAGGAGATATTCCTGAAATTGAGTGGTGGGACTCTTACATAATCCCCAA TGGCTTTGATCTTACAGAGGAAAATCCCAAGAGAGAAGATTATTTTGGAATCACAAATCTTGTTGAACAT CCAGCCCAGCTCAATCCTCCAGTTGACAATGACACACCAGTTACTCTGGGAGTATATCTTACCAAGAAGG AACAGAAAAAACTTCGGAGACAAACAAGGAGGGAAGCACAGAAGGAACTACAAGAAAAAGTCAGGCTGGG CCTGATGCCTCCTCCAGAACCCAAAGTGAGAATTTCTAATTTGATGCGAGTATTAGGAACAGAAGCTGTT CAAGACCCCACGAAGGTAGAAGCCCACGTCAGAGCTCAGATGGCAAAAAGACAGAAAGCGCATGAAGAGG CCAACGCTGCCCGAAAACTCACAGCAGAACAGAGAAAGGTCAAGAAAATTAAAAAGCTTAAAGAAGACAT TTCACAGGGGGTACACATATCTGTATATAGAGTTCGAAATTTGAGCAACCCAGCCAAGAAGTTCAAGATT GAAGCCAATGCTGGGCAACTGTACCTGACAGGGGTGGTGGTACTGCACAAGGATGTCAACGTGGTAGTAG TGGAAGGGGGCCCCAAGGCCCAGAAGAAATTTAAGCGTCTTATGCTGCATCGGATAAAGTGGGATGAACA GACATCTAACACAAAGGGAGATGATGATGAGGAGTCTGATGAGGAAGCTGTGAAGAAAACCAACAAATGT GTACTAGTCTGGGAGGGTACAGCCAAAGACCGGAGCTTTGGAGAGATGAAGTTTAAACAGTGTCCTACAG AGAACATGGCTCGTGAGCATTTCAAAAAGCATGGGGCTGAACACTACTGGGACCTTGCGCTGAGTGAATC TGTGTTAGAGTCCACTGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201365 protein sequence
Red=Cloning site Green=Tags(s) MALSKRELDELKPWIEKTVKRVLGFSEPTVVTAALNCVGKGMDKKKAADHLKPFLDDSTLRFVDKLFEAV EEGRSSRHSKSSSDRSRKRELKEVFGDDSEISKESSGVKKRRIPRFEEVEEEPEVIPGPPSESPGMLTKL QIKQMMEAATRQIEERKKQLSFISPPTPQPKTPSSSQPERLPIGNTIQPSQAATFMNDAIEKARKAAELQ ARIQAQLALKPGLIGNANMVGLANLHAMGIAPPKVELKDQTKPTPLILDEQGRTVDATGKEIELTHRMPT LKANIRAVKREQFKQQLKEKPSEDMESNTFFDPRVSIAPSQRQRRTFKFHDKGKFEKIAQRLRTKAQLEK LQAEISQAARKTGIHTSTRLALIAPKKELKEGDIPEIEWWDSYIIPNGFDLTEENPKREDYFGITNLVEH PAQLNPPVDNDTPVTLGVYLTKKEQKKLRRQTRREAQKELQEKVRLGLMPPPEPKVRISNLMRVLGTEAV QDPTKVEAHVRAQMAKRQKAHEEANAARKLTAEQRKVKKIKKLKEDISQGVHISVYRVRNLSNPAKKFKI EANAGQLYLTGVVVLHKDVNVVVVEGGPKAQKKFKRLMLHRIKWDEQTSNTKGDDDEESDEEAVKKTNKC VLVWEGTAKDRSFGEMKFKQCPTENMAREHFKKHGAEHYWDLALSESVLESTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004698 |
| ORF Size | 2049 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004698.4 |
| RefSeq Size | 2480 bp |
| RefSeq ORF | 2052 bp |
| Locus ID | 9129 |
| UniProt ID | O43395 |
| Domains | PWI |
| Protein Pathways | Spliceosome |
| MW | 77.5 kDa |
| Gene Summary | The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201365L3 | Lenti ORF clone of Human PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae) (PRPF3), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC201365L4 | Lenti ORF clone of Human PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae) (PRPF3), mGFP tagged |
CNY 7,700.00 |
|
| RG201365 | PRPF3 (tGFP-tagged) - Human PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae) (PRPF3) |
CNY 6,370.00 |
|
| SC319472 | PRPF3 (untagged)-Human PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae) (PRPF3) |
CNY 2,950.00 |
