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Annexin VII (ANXA7) (NM_001156) Human Tagged ORF Clone
CAT＃: RC201226
ANXA7 (Myc-DDK-tagged)-Human annexin A7 (ANXA7), transcript variant 1
ORF Plasmid: tGFP
"NM_001156" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ANX7; SNX; SYNEXIN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201226 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATACCCAGGCTATCCCCCAACAGGCTACCCACCTTTCCCTGGATATCCTCCTGCAGGTCAGGAGT CATCTTTTCCCCCTTCTGGTCAGTATCCTTATCCTAGTGGCTTTCCTCCAATGGGAGGAGGTGCCTACCC ACAAGTGCCAAGTAGTGGCTACCCAGGAGCTGGAGGCTACCCTGCGCCTGGAGGTTATCCAGCCCCTGGA GGCTATCCTGGTGCCCCACAGCCAGGGGGAGCTCCATCCTATCCCGGAGTTCCTCCAGGCCAAGGATTTG GAGTCCCACCAGGTGGAGCAGGCTTTTCTGGGTATCCACAGCCACCTTCACAGTCTTATGGAGGTGGTCC AGCACAGGTTCCACTACCTGGTGGCTTTCCTGGAGGACAGATGCCTTCTCAGTATCCTGGAGGACAACCT ACTTACCCTAGTCAGCCTGCCACAGTGACTCAGGTCACTCAAGGAACTATCCGACCAGCTGCCAACTTCG ATGCTATAAGAGATGCAGAAATTCTTCGTAAGGCAATGAAGGGTTTTGGGACAGATGAGCAGGCAATTGT GGATGTGGTGGCCAACCGTTCCAATGATCAGAGGCAAAAAATTAAAGCAGCATTTAAGACCTCCTATGGC AAGGATTTAATCAAAGATCTCAAATCAGAGTTAAGTGGAAATATGGAAGAACTGATCCTGGCCCTCTTCA TGCCTCCTACGTATTACGATGCCTGGAGCTTACGGAAAGCAATGCAGGGAGCAGGAACTCAGGAACGTGT ATTGATTGAGATTTTGTGCACAAGAACAAATCAGGAAATCCGAGAAATTGTCAGATGTTATCAGTCAGAA TTTGGACGAGACCTTGAAAAGGACATTAGGTCAGATACATCAGGACATTTTGAACGTTTACTTGTGTCCA TGTGCCAGGGAAATCGTGATGAGAACCAGAGTATAAACCACCAAATGGCTCAGGAAGATGCTCAGCGTCT CTATCAAGCTGGTGAGGGGAGACTAGGGACCGATGAATCTTGCTTTAACATGATCCTTGCCACAAGAAGC TTTCCTCAGCTGAGAGCTACCATGGAGGCTTATTCTAGGATGGCTAATCGAGACTTGTTAAGCAGTGTGA GCCGTGAGTTTTCCGGATATGTAGAAAGTGGTTTGAAGACCATCTTGCAGTGTGCCCTGAACCGCCCTGC CTTCTTTGCTGAGAGGCTCTACTATGCTATGAAAGGTGCTGGCACAGATGACTCCACCCTGGTCCGGATT GTGGTCACTCGAAGTGAGATTGACCTTGTACAAATAAAACAGATGTTCGCTCAGATGTATCAGAAGACTC TGGGCACAATGATTGCAGGTGACACGAGTGGAGATTACCGAAGACTTCTTCTGGCTATTGTGGGCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201226 protein sequence
Red=Cloning site Green=Tags(s) MSYPGYPPTGYPPFPGYPPAGQESSFPPSGQYPYPSGFPPMGGGAYPQVPSSGYPGAGGYPAPGGYPAPG GYPGAPQPGGAPSYPGVPPGQGFGVPPGGAGFSGYPQPPSQSYGGGPAQVPLPGGFPGGQMPSQYPGGQP TYPSQPATVTQVTQGTIRPAANFDAIRDAEILRKAMKGFGTDEQAIVDVVANRSNDQRQKIKAAFKTSYG KDLIKDLKSELSGNMEELILALFMPPTYYDAWSLRKAMQGAGTQERVLIEILCTRTNQEIREIVRCYQSE FGRDLEKDIRSDTSGHFERLLVSMCQGNRDENQSINHQMAQEDAQRLYQAGEGRLGTDESCFNMILATRS FPQLRATMEAYSRMANRDLLSSVSREFSGYVESGLKTILQCALNRPAFFAERLYYAMKGAGTDDSTLVRI VVTRSEIDLVQIKQMFAQMYQKTLGTMIAGDTSGDYRRLLLAIVGQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001156 |
| ORF Size | 1398 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001156.5 |
| RefSeq Size | 2146 bp |
| RefSeq ORF | 1401 bp |
| Locus ID | 310 |
| UniProt ID | P20073 |
| Domains | annexin |
| MW | 50.3 kDa |
| Gene Summary | Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201226L1 | Lenti ORF clone of Human annexin A7 (ANXA7), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201226L2 | Lenti ORF clone of Human annexin A7 (ANXA7), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC201226L3 | Lenti ORF clone of Human annexin A7 (ANXA7), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201226L4 | Lenti ORF clone of Human annexin A7 (ANXA7), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG201226 | ANXA7 (tGFP-tagged) - Human annexin A7 (ANXA7), transcript variant 1 |
CNY 4,370.00 |
|
| SC126802 | ANXA7 (untagged)-Human annexin A7 (ANXA7), transcript variant 1 |
CNY 2,950.00 |
|
| SC320695 | ANXA7 (untagged)-Human annexin A7 (ANXA7), transcript variant 1 |
CNY 2,950.00 |
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