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SF3A1 (NM_005877) Human Tagged ORF Clone
CAT＃: RC201098
SF3A1 (Myc-DDK-tagged)-Human splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1
ORF Plasmid: tGFP
"NM_005877" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PRP21; PRPF21; SAP114; SF3A120 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201098 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGCCGGACCCGTGCAGGCGGTGCCCCCGCCGCCGCCCGTGCCCACGGAGCCCAAACAGCCCACAG AAGAAGAAGCATCTTCAAAGGAGGATTCTGCACCTTCTAAGCCAGTTGTGGGGATTATTTACCCTCCTCC AGAGGTCAGAAATATTGTTGACAAGACTGCCAGCTTTGTGGCCAGAAACGGGCCTGAATTTGAAGCTAGG ATCCGACAGAACGAGATCAACAACCCCAAGTTCAACTTTCTGAACCCCAATGACCCTTACCATGCCTACT ACCGCCACAAGGTCAGCGAGTTCAAGGAAGGGAAGGCTCAGGAGCCGTCCGCCGCCATCCCCAAGGTCAT GCAGCAGCAGCAGCAGACCACCCAGCAGCAGCTGCCCCAGAAGGTCCAAGCCCAAGTAATCCAAGAGACC ATCGTGCCCAAAGAGCCTCCTCCTGAGTTTGAGTTCATTGCTGATCCTCCCTCTATCTCAGCCTTCGACT TGGATGTGGTGAAGCTGACGGCTCAGTTTGTGGCCAGGAATGGGCGCCAGTTTCTGACCCAGCTGATGCA GAAAGAGCAGCGCAACTACCAGTTTGACTTTCTCCGCCCACAGCACAGCCTCTTCAACTACTTCACGAAG CTAGTGGAACAGTACACCAAGATCTTGATTCCACCCAAAGGTTTATTTTCAAAGCTCAAGAAAGAGGCTG AAAACCCCCGAGAAGTTTTGGATCAGGTGTGTTACCGAGTGGAATGGGCCAAATTCCAGGAACGTGAGAG GAAGAAGGAAGAAGAGGAGAAGGAGAAGGAGCGGGTGGCCTATGCTCAGATCGACTGGCATGATTTTGTG GTGGTGGAAACAGTGGACTTCCAACCCAATGAGCAAGGGAACTTCCCTCCCCCCACCACGCCAGAGGAGC TGGGGGCCCGAATCCTCATTCAGGAGCGCTATGAAAAGTTTGGGGAGAGTGAGGAAGTTGAGATGGAGGT CGAGTCTGATGAGGAGGATGACAAACAGGAGAAGGCGGAGGAGCCTCCTTCCCAGCTGGACCAGGACACC CAAGTACAAGATATGGATGAGGGTTCAGATGATGAAGAAGAAGGGCAGAAAGTGCCCCCACCCCCAGAGA CACCCATGCCTCCACCTCTGCCCCCAACTCCAGACCAAGTCATTGTCCGCAAGGATTATGATCCCAAAGC CTCCAAGCCCTTGCCTCCAGCCCCTGCTCCAGATGAGTATCTTGTGTCCCCCATTACTGGGGAGAAGATC CCCGCCAGCAAAATGCAGGAACACATGCGCATTGGACTTCTTGACCCTCGCTGGCTGGAGCAGCGGGATC GCTCCATCCGTGAGAAGCAGAGCGATGATGAGGTGTACGCACCAGGTCTGGATATTGAGAGCAGCTTGAA GCAGTTGGCTGAGCGGCGTACTGACATCTTCGGTGTAGAGGAAACAGCCATTGGTAAGAAGATCGGTGAG GAGGAGATCCAGAAGCCAGAGGAAAAGGTGACCTGGGATGGCCACTCAGGCAGCATGGCCCGGACCCAGC AGGCTGCCCAGGCCAACATCACCCTCCAGGAGCAGATTGAGGCCATTCACAAGGCCAAAGGCCTGGTGCC AGAGGATGACACTAAAGAGAAGATTGGCCCCAGCAAGCCCAATGAAATCCCTCAACAGCCACCGCCACCA TCTTCAGCCACCAACATCCCCAGCTCGGCTCCACCCATCACTTCAGTGCCCCGACCACCCACAATGCCAC CTCCAGTTCGTACTACAGTTGTCTCCGCAGTACCCGTCATGCCCCGGCCCCCAATGGCATCTGTGGTCCG GCTGCCCCCAGGCTCAGTGATCGCCCCCATGCCGCCCATCATCCACGCGCCCAGAATCAACGTGGTGCCC ATGCCTCCCTCGGCCCCTCCTATTATGGCCCCCCGCCCACCCCCCATGATTGTGCCAACAGCCTTTGTGC CTGCTCCACCTGTGGCACCTGTCCCAGCTCCAGCCCCAATGCCCCCTGTGCATCCCCCACCTCCCATGGA AGATGAGCCCACCTCCAAAAAACTGAAGACAGAGGACAGCCTCATGCCAGAGGAGGAGTTCCTGCGCAGA AACAAGGGTCCAGTGTCCATCAAAGTCCAGGTGCCCAACATGCAGGATAAGACGGAATGGAAACTGAATG GGCAGGTGCTGGTCTTCACCCTCCCACTCACGGACCAGGTCTCTGTCATTAAGGTGAAGATTCATGAAGC CACAGGCATGCCTGCAGGGAAACAGAAGCTACAGTATGAGGGTATCTTCATCAAAGATTCCAACTCACTG GCTTACTACAACATGGCCAATGGCGCAGTCATCCACCTGGCCCTCAAGGAGAGAGGCGGGAGGAAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201098 protein sequence
Red=Cloning site Green=Tags(s) MPAGPVQAVPPPPPVPTEPKQPTEEEASSKEDSAPSKPVVGIIYPPPEVRNIVDKTASFVARNGPEFEAR IRQNEINNPKFNFLNPNDPYHAYYRHKVSEFKEGKAQEPSAAIPKVMQQQQQTTQQQLPQKVQAQVIQET IVPKEPPPEFEFIADPPSISAFDLDVVKLTAQFVARNGRQFLTQLMQKEQRNYQFDFLRPQHSLFNYFTK LVEQYTKILIPPKGLFSKLKKEAENPREVLDQVCYRVEWAKFQERERKKEEEEKEKERVAYAQIDWHDFV VVETVDFQPNEQGNFPPPTTPEELGARILIQERYEKFGESEEVEMEVESDEEDDKQEKAEEPPSQLDQDT QVQDMDEGSDDEEEGQKVPPPPETPMPPPLPPTPDQVIVRKDYDPKASKPLPPAPAPDEYLVSPITGEKI PASKMQEHMRIGLLDPRWLEQRDRSIREKQSDDEVYAPGLDIESSLKQLAERRTDIFGVEETAIGKKIGE EEIQKPEEKVTWDGHSGSMARTQQAAQANITLQEQIEAIHKAKGLVPEDDTKEKIGPSKPNEIPQQPPPP SSATNIPSSAPPITSVPRPPTMPPPVRTTVVSAVPVMPRPPMASVVRLPPGSVIAPMPPIIHAPRINVVP MPPSAPPIMAPRPPPMIVPTAFVPAPPVAPVPAPAPMPPVHPPPPMEDEPTSKKLKTEDSLMPEEEFLRR NKGPVSIKVQVPNMQDKTEWKLNGQVLVFTLPLTDQVSVIKVKIHEATGMPAGKQKLQYEGIFIKDSNSL AYYNMANGAVIHLALKERGGRKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005877 |
| ORF Size | 2379 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005877.6 |
| RefSeq Size | 5159 bp |
| RefSeq ORF | 2382 bp |
| Locus ID | 10291 |
| UniProt ID | Q15459 |
| Domains | Surp, UBQ |
| Protein Families | Druggable Genome |
| Protein Pathways | Spliceosome |
| MW | 88.9 kDa |
| Gene Summary | This gene encodes a subunit of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer is a component of the mature U2 small nuclear ribonucleoprotein particle (snRNP). U2 small nuclear ribonucleoproteins play a critical role in spliceosome assembly and pre-mRNA splicing. [provided by RefSeq, Aug 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201098L1 | Lenti ORF clone of Human splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201098L2 | Lenti ORF clone of Human splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC201098L3 | Lenti ORF clone of Human splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201098L4 | Lenti ORF clone of Human splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG201098 | SF3A1 (tGFP-tagged) - Human splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1 |
CNY 4,370.00 |
|
| SC321295 | SF3A1 (untagged)-Human splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1 |
CNY 2,950.00 |
