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KBTBD6 (NM_152903) Human Tagged ORF Clone
CAT＃: RC200950
KBTBD6 (Myc-DDK-tagged)-Human kelch repeat and BTB (POZ) domain containing 6 (KBTBD6)
ORF Plasmid: tGFP
"NM_152903" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200950 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTCCCGGGAAGACGCCCCGCGCTCTCGCCGCCTAGCCAGTCCCCGTGGTGGGAAGCGGCCCAAGA AGATTCACAAACCCACAGTTTCGGCCTTTTTCACGGGTCCAGAGGAATTAAAGGACACGGCCCATTCTGC AGCCCTGCTGGCACAGCTCAAGTCCTTCTACGATGCGCGGCTGCTGTGTGATGTGACCATCGAGGTGGTG ACGCCTGGCAGCGGGCCTGGCACGGGTCGCCTGTTCCCCTGCAACCGCAATGTGCTGGCCGCGGCATGTC CCTACTTCAAGAGCATGTTCACAGGTGGCATGTACGAGAGCCAGCAGGCCAGCGTGACCATGCACGATGT GGACGCCGAGTCCTTCGAGGTGTTGGTCGACTACTGCTACACGGGTCGTGTGTCTCTCAGTGAGGCCAAC GTGGAGCGCCTGTACGCGGCCTCCGACATGCTACAGCTGGAATATGTGCGGGAAGCCTGTGCCTCCTTCT TAGCCCGACGTCTTGACCTGACCAACTGCACCGCCATCCTCAAGTTTGCAGATGCCTTTGGCCATCGCAA GCTGCGATCCCAGGCCCAGTCCTATATAGCTCAGAACTTCAAGCAACTCAGCCACATGGGTTCAATTCGG GAGGAGACTCTAGCAGATCTGACCCTGGCCCAGCTGCTGGCTGTCCTGCGCTTGGATAGTCTGGACGTGG AGAGTGAGCAGACAGTGTGCCATGTGGCAGTGCAGTGGCTGGAGGCTGCTCCCAAAGAGCGGGGTCCCAG TGCTGCAGAAGTCTTCAAGTGCGTGCGCTGGATGCACTTCACTGAAGAAGATCAGGACTACTTAGAAGGG CTGCTGACCAAGCCCATCGTGAAGAAGTACTGCCTGGACGTTATTGAAGGGGCCCTGCAGATGCGCTATG GTGACCTGTTGTACAAGTCTCTGGTGCCAGTGCCAAACAGCAGCAGCAGCAGTAGCAGCAGCAACTCTCT TGTATCTGCAGCAGAAAATCCACCCCAGAGACTGGGTATGTGTGCCAAGGAGATGGTGATCTTCTTTGGA CACCCCAGAGATCCCTTTCTCTGCTGTGATCCATACTCGGGGGACCTTTACAAAGTGCCGTCACCTTTGA CCTGTCTGGCTCACACTAGGACTGTCACCACTCTAGCTGTCTGTATCTCTCCTGACCATGACATCTATCT AGCTGCTCAGCCCAGGACAGACCTCTGGGTGTATAAACCAGCTCAGAATAGTTGGCAGCAACTTGCAGAT CGCTTGCTGTGTCGTGAGGGCATGGATGTGGCATATCTCAATGGCTATATCTACATTTTGGGGGGGCGAG ACCCTATTACTGGAGTTAAGTTGAAGGAAGTGGAATGCTACAATGTTAAGAGAAACCAGTGGGCATTGGT GGCTCCACTGCCCCATTCTTTTTTATCCTTTGACCTAATGGTAATTCGAGACTATCTCTATGCTCTCAAC AGTAAGCGCATGTTCTGTTATGATCCTAGCCACAATATGTGGCTGAAGTGCGTTTCTCTGAAGCGCAATG ACTTTCAGGAAGCCTGCGTCTTCAATGAGGAGATCTATTGTATCTGTGATATCCCAGTCATGAAGGTCTA CAACCCAGTTAGGGCAGAATGGAGGCAAATGAATAATATTCCCTTGGTCTCAGAGACCAACAACTACAGA ATTATCAAGCATGGCCAAAAATTGTTGCTCATCACCTCTCGCACCCCACAGTGGAAAAAGAACCGGGTGA CTGTGTATGAATATGATATTAGGGGAGACCAATGGATTAATATAGGTACCACATTAGGCCTCTTGCAGTT TGATTCTAACTTTTTTTGCCTCTCTGCTCGTGTTTATCCTTCCTGCCTTGAACCTGGTCAGAGTTTCCTC ACTGAAGAAGAAGAAATACCAAGTGAGTCTAGCACTGAATGGGACTTAGGTGGATTCAGTGAGCCAGACT CTGAGTCAGGAAGTTCAAGTTCTCTTTCTGATGATGATTTTTGGGTGCGTGTAGCGCCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200950 protein sequence
Red=Cloning site Green=Tags(s) MQSREDAPRSRRLASPRGGKRPKKIHKPTVSAFFTGPEELKDTAHSAALLAQLKSFYDARLLCDVTIEVV TPGSGPGTGRLFPCNRNVLAAACPYFKSMFTGGMYESQQASVTMHDVDAESFEVLVDYCYTGRVSLSEAN VERLYAASDMLQLEYVREACASFLARRLDLTNCTAILKFADAFGHRKLRSQAQSYIAQNFKQLSHMGSIR EETLADLTLAQLLAVLRLDSLDVESEQTVCHVAVQWLEAAPKERGPSAAEVFKCVRWMHFTEEDQDYLEG LLTKPIVKKYCLDVIEGALQMRYGDLLYKSLVPVPNSSSSSSSSNSLVSAAENPPQRLGMCAKEMVIFFG HPRDPFLCCDPYSGDLYKVPSPLTCLAHTRTVTTLAVCISPDHDIYLAAQPRTDLWVYKPAQNSWQQLAD RLLCREGMDVAYLNGYIYILGGRDPITGVKLKEVECYNVKRNQWALVAPLPHSFLSFDLMVIRDYLYALN SKRMFCYDPSHNMWLKCVSLKRNDFQEACVFNEEIYCICDIPVMKVYNPVRAEWRQMNNIPLVSETNNYR IIKHGQKLLLITSRTPQWKKNRVTVYEYDIRGDQWINIGTTLGLLQFDSNFFCLSARVYPSCLEPGQSFL TEEEEIPSESSTEWDLGGFSEPDSESGSSSSLSDDDFWVRVAPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152903 |
| ORF Size | 2022 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_152903.4, NP_690867.3 |
| RefSeq Size | 5228 bp |
| RefSeq ORF | 2025 bp |
| Locus ID | 89890 |
| UniProt ID | Q86V97 |
| Domains | BTB, Kelch |
| MW | 76.1 kDa |
| Gene Summary | As part of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex functions as a substrate adapter for the RAC1 guanine exchange factor (GEF) TIAM1, mediating its 'Lys-48' ubiquitination and proteasomal degradation (PubMed:25684205). By controlling this ubiquitination, regulates RAC1 signal transduction and downstream biological processes including the organization of the cytoskeleton, cell migration and cell proliferation (PubMed:25684205). Ubiquitination of TIAM1 requires the membrane-associated protein GABARAP which may restrict locally the activity of the complex (PubMed:25684205).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200950L3 | Lenti ORF clone of Human kelch repeat and BTB (POZ) domain containing 6 (KBTBD6), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200950L4 | Lenti ORF clone of Human kelch repeat and BTB (POZ) domain containing 6 (KBTBD6), mGFP tagged |
CNY 5,890.00 |
|
| RG200950 | KBTBD6 (tGFP-tagged) - Human kelch repeat and BTB (POZ) domain containing 6 (KBTBD6) |
CNY 4,370.00 |
|
| SC100640 | KBTBD6 (untagged)-Human kelch repeat and BTB (POZ) domain containing 6 (KBTBD6) |
CNY 7,220.00 |
