KCTD14 (NM_023930) Human Tagged ORF Clone

CAT#: RC200745

KCTD14 (Myc-DDK-tagged)-Human potassium channel tetramerisation domain containing 14 (KCTD14)

ORF Plasmid: DDK tGFP



  "NM_023930" in other vectors (5)

Need custom modification / cloning service?
Get a free quote

CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


KCTD14 mouse monoclonal antibody, clone OTI1D11 (formerly 1D11)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200745 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTACTGTTGTGGAGCTGAACGTCGGGGGTGAGTTCCACACCACCACCCTGGGTACCCTGAGGAAGT
TTCCGGGCTCAAAGCTGGCAGAGATGTTCTCTAGCTTAGCCAAGGCCTCCACGGACGCGGAGGGCCGCTT
CTTCATCGACCGCCCCAGCACCTATTTCAGACCCATCCTGGACTACCTGCGCACTGGGCAAGTGCCCACA
CAGCACATCCCTGAAGTGTACCGTGAGGCTCAGTTCTACGAAATCAAGCCTTTGGTCAAGCTGCTGGAGG
ACATGCCACAGATCTTTGGTGAGCAGGTGTCTCGGAAGCAGTTTTTGCTGCAAGTGCCGGGCTACAGCGA
GAACCTGGAGCTCATGGTGCGCCTGGCACGTGCAGAAGCCATAACAGCACGGAAGTCCAGCGTGCTTGTG
TGCCTGGTGGAAACTGAGGAGCAGGATGCATATTATTCAGAGGTCCTGTGTTTTCTGCAGGATAAGAAGA
TGTTCAAGTCTGTTGTCAAGTTTGGGCCCTGGAAGGCGGTCCTAGACAACAGCGACCTCATGCACTGCCT
GGAGATGGACATTAAGGCCCAGGGGTACAAGGTATTCTCCAAGTTCTACCTGACGTACCCCACCAAAAGA
AACGAATTCCATTTTAACATTTATTCATTCACCTTCACCTGGTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200745 protein sequence
Red=Cloning site Green=Tags(s)

MSTVVELNVGGEFHTTTLGTLRKFPGSKLAEMFSSLAKASTDAEGRFFIDRPSTYFRPILDYLRTGQVPT
QHIPEVYREAQFYEIKPLVKLLEDMPQIFGEQVSRKQFLLQVPGYSENLELMVRLARAEAITARKSSVLV
CLVETEEQDAYYSEVLCFLQDKKMFKSVVKFGPWKAVLDNSDLMHCLEMDIKAQGYKVFSKFYLTYPTKR
NEFHFNIYSFTFTWW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_023930
ORF Size 675 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_023930.2, NP_076419.1
RefSeq Size 1688 bp
RefSeq ORF 768 bp
Locus ID 65987
UniProt ID Q9BQ13
Domains BTB, K_tetra
Protein Families Ion Channels: Other
MW 26.2 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...