United States
Germany
Japan
United Kingdom
China
nSMase (SMPD2) (NM_003080) Human Tagged ORF Clone
CAT＃: RC200417
SMPD2 (Myc-DDK-tagged)-Human sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase) (SMPD2)
ORF Plasmid: tGFP
"NM_003080" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 5,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ISC1; NSMASE; NSMASE1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200417 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCCAACTTCTCCCTGCGACTGCGGATCTTCAACCTCAACTGCTGGGGCATTCCGTACTTGAGCA AGCACCGGGCCGACCGCATGAGGCGCCTGGGAGACTTTCTGAACCAGGAGAGCTTCGACCTGGCTTTGCT GGAGGAGGTGTGGAGTGAGCAGGACTTCCAGTACCTGAGACAGAAGCTGTCACCTACCTACCCAGCTGCA CACCACTTCCGGAGCGGAATCATTGGCAGTGGCCTCTGTGTCTTCTCCAAACATCCAATCCAGGAGCTTA CCCAGCACATCTACACTCTCAATGGCTACCCCTACATGATCCATCATGGTGACTGGTTCAGTGGGAAGGC TGTGGGGCTGCTGGTGCTCCATCTAAGTGGCATGGTGCTCAACGCCTATGTGACCCATCTCCATGCCGAA TACAATCGACAGAAGGACATCTACCTAGCACATCGTGTGGCCCAAGCTTGGGAATTGGCCCAGTTCATCC ACCACACATCCAAGAAGGCAGACGTGGTTCTGTTGTGTGGAGACCTCAACATGCACCCAGAAGACCTGGG CTGCTGCCTGCTGAAGGAGTGGACAGGGCTTCATGATGCCTATCTTGAAACTCGGGACTTCAAGGGCTCT GAGGAAGGCAACACAATGGTACCCAAGAACTGCTACGTCAGCCAGCAGGAGCTGAAGCCATTTCCCTTTG GTGTCCGCATTGACTACGTGCTTTACAAGGCAGTTTCTGGGTTTTACATCTCCTGTAAGAGTTTTGAAAC CACTACAGGCTTTGACCCTCACAGTGGCACCCCCCTCTCTGATCATGAAGCCCTGATGGCTACTCTGTTT GTGAGGCACAGCCCCCCACAGCAGAACCCCAGCTCTACCCACGGACCAGCAGAGAGGTCGCCGTTGATGT GTGTGCTAAAGGAGGCCTGGACGGAGCTGGGTCTGGGCATGGCTCAGGCTCGCTGGTGGGCCACCTTCGC TAGCTATGTGATTGGCCTGGGGCTGCTTCTCCTGGCACTGCTGTGTGTCCTGGCGGCTGGAGGAGGGGCC GGGGAAGCTGCCATACTGCTCTGGACCCCCAGTGTAGGGCTGGTGCTGTGGGCAGGTGCATTCTACCTCT TCCACGTACAGGAGGTCAATGGCTTATATAGGGCCCAGGCTGAGCTCCAGCATGTGCTAGGAAGGGCAAG GGAGGCCCAGGATCTGGGCCCAGAGCCTCAGCCAGCCCTACTCCTGGGGCAGCAGGAGGGGGACAGAACT AAAGAACAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200417 protein sequence
Red=Cloning site Green=Tags(s) MKPNFSLRLRIFNLNCWGIPYLSKHRADRMRRLGDFLNQESFDLALLEEVWSEQDFQYLRQKLSPTYPAA HHFRSGIIGSGLCVFSKHPIQELTQHIYTLNGYPYMIHHGDWFSGKAVGLLVLHLSGMVLNAYVTHLHAE YNRQKDIYLAHRVAQAWELAQFIHHTSKKADVVLLCGDLNMHPEDLGCCLLKEWTGLHDAYLETRDFKGS EEGNTMVPKNCYVSQQELKPFPFGVRIDYVLYKAVSGFYISCKSFETTTGFDPHSGTPLSDHEALMATLF VRHSPPQQNPSSTHGPAERSPLMCVLKEAWTELGLGMAQARWWATFASYVIGLGLLLLALLCVLAAGGGA GEAAILLWTPSVGLVLWAGAFYLFHVQEVNGLYRAQAELQHVLGRAREAQDLGPEPQPALLLGQQEGDRT KEQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003080 |
| ORF Size | 1269 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003080.3 |
| RefSeq Size | 1697 bp |
| RefSeq ORF | 1272 bp |
| Locus ID | 6610 |
| UniProt ID | O60906 |
| Domains | Exo_endo_phos |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
| MW | 47.6 kDa |
| Gene Summary | This gene encodes a protein which was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase. [provided by RefSeq, Oct 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200417L3 | Lenti ORF clone of Human sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase) (SMPD2), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200417L4 | Lenti ORF clone of Human sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase) (SMPD2), mGFP tagged |
CNY 5,890.00 |
|
| RG200417 | SMPD2 (tGFP-tagged) - Human sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase) (SMPD2) |
CNY 4,370.00 |
|
| SC319400 | SMPD2 (untagged)-Human sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase) (SMPD2) |
CNY 3,656.00 |
