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2 Hydroxy phytanoyl CoA lyase (HACL1) (NM_012260) Human Tagged ORF Clone
CAT＃: RC200197
HACL1 (Myc-DDK-tagged)-Human 2-hydroxyacyl-CoA lyase 1 (HACL1)
ORF Plasmid: tGFP
"NM_012260" in other vectors (4)
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CNY 4,304.00
CNY 5,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2-HPCL; HPCL; HPCL2; PHYH2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200197 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGACAGTAACTTCGCAGAGCGCAGCGAGGAGCAGGTGTCTGGTGCTAAAGTCATCGCTCAGGCCC TGAAAACGCAAGATGTGGAGTACATATTTGGCATCGTAGGCATCCCAGTGACCGAAATCGCCATTGCTGC CCAGCAGCTAGGCATCAAGTACATCGGGATGAGGAATGAGCAAGCGGCTTGTTATGCTGCCTCCGCGATT GGATATCTGACAAGCAGGCCAGGAGTCTGCCTTGTTGTTTCTGGCCCAGGTCTCATCCATGCCTTGGGCG GTATGGCAAATGCAAACATGAACTGCTGGCCCTTGCTTGTGATTGGTGGTTCCTCTGAAAGAAACCAAGA AACAATGGGAGCTTTCCAGGAGTTTCCTCAGGTTGAAGCTTGTAGATTATATACCAAGTTCTCTGCCCGC CCAAGCAGCATAGAAGCTATTCCTTTTGTTATTGAAAAGGCAGTGAGAAGCAGTATCTATGGTCGTCCAG GTGCTTGCTATGTTGACATACCAGCAGATTTTGTGAACCTTCAGGTGAATGTGAATTCTATAAAGTACAT GGAACGCTGCATGTCACCTCCTATTAGCATGGCAGAAACCTCTGCTGTGTGCACGGCGGCTTCTGTTATT AGGAATGCCAAACAACCCCTTCTTATCATCGGGAAAGGTGCTGCTTACGCTCATGCAGAAGAGAGTATCA AGAAATTGGTGGAGCAATATAAACTGCCATTTTTGCCCACCCCTATGGGGAAGGGTGTTGTCCCTGACAA CCATCCATACTGTGTAGGTGCAGCCAGATCCAGGGCTTTGCAATTTGCTGATGTAATTGTGTTATTTGGT GCCAGACTAAATTGGATTTTACATTTTGGACTGCCTCCAAGATATCAGCCAGATGTGAAGTTTATCCAGG TTGATATCTGTGCAGAAGAATTGGGGAATAATGTAAAGCCCGCTGTTACTTTGCTAGGAAACATACATGC TGTCACTAAGCAGCTTTTAGAGGAACTTGATAAAACACCATGGCAGTATCCTCCAGAGAGCAAGTGGTGG AAAACTCTGAGAGAAAAAATGAAGAGCAATGAAGCTGCATCCAAGGAACTAGCTTCTAAAAAATCCCTGC CTATGAATTATTACACAGTATTCTACCATGTTCAAGAACAACTACCTAGAGACTGTTTCGTGGTAAGTGA AGGAGCAAATACTATGGACATTGGACGGACTGTGCTTCAGAACTACCTTCCTCGTCACAGGCTTGATGCT GGTACTTTCGGAACAATGGGAGTTGGTTTGGGATTTGCTATTGCAGCTGCCGTGGTGGCTAAAGATAGAA GCCCTGGGCAATGGATCATCTGTGTGGAAGGAGACAGTGCATTTGGGTTTTCTGGCATGGAGGTAGAAAC CATCTGCAGGTACAACTTGCCAATCATACTGTTGGTAGTGAATAACAATGGAATTTACCAAGGTTTTGAT ACAGATACTTGGAAAGAAATGTTAAAATTTCAAGATGCTACTGCAGTGGTCCCTCCAATGTGTTTGCTGC CAAATTCACATTATGAGCAAGTCATGACTGCATTTGGAGGCAAAGGGTATTTTGTACAAACACCAGAAGA ACTCCAAAAATCCCTGAGGCAGAGCCTAGCAGACACAACTAAACCTTCTCTTATCAACATCATGATTGAG CCCCAAGCCACACGGAAGGCCCAGGATTTTCATTGGCTGACCCGCTCTAATATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200197 protein sequence
Red=Cloning site Green=Tags(s) MPDSNFAERSEEQVSGAKVIAQALKTQDVEYIFGIVGIPVTEIAIAAQQLGIKYIGMRNEQAACYAASAI GYLTSRPGVCLVVSGPGLIHALGGMANANMNCWPLLVIGGSSERNQETMGAFQEFPQVEACRLYTKFSAR PSSIEAIPFVIEKAVRSSIYGRPGACYVDIPADFVNLQVNVNSIKYMERCMSPPISMAETSAVCTAASVI RNAKQPLLIIGKGAAYAHAEESIKKLVEQYKLPFLPTPMGKGVVPDNHPYCVGAARSRALQFADVIVLFG ARLNWILHFGLPPRYQPDVKFIQVDICAEELGNNVKPAVTLLGNIHAVTKQLLEELDKTPWQYPPESKWW KTLREKMKSNEAASKELASKKSLPMNYYTVFYHVQEQLPRDCFVVSEGANTMDIGRTVLQNYLPRHRLDA GTFGTMGVGLGFAIAAAVVAKDRSPGQWIICVEGDSAFGFSGMEVETICRYNLPIILLVVNNNGIYQGFD TDTWKEMLKFQDATAVVPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQKSLRQSLADTTKPSLINIMIE PQATRKAQDFHWLTRSNM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012260 |
| ORF Size | 1734 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012260.4 |
| RefSeq Size | 2287 bp |
| RefSeq ORF | 1737 bp |
| Locus ID | 26061 |
| UniProt ID | Q9UJ83 |
| Domains | TPP_enzymes |
| MW | 63.7 kDa |
| Gene Summary | Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200197L3 | Lenti ORF clone of Human 2-hydroxyacyl-CoA lyase 1 (HACL1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200197L4 | Lenti ORF clone of Human 2-hydroxyacyl-CoA lyase 1 (HACL1), mGFP tagged |
CNY 5,890.00 |
|
| RG200197 | HACL1 (tGFP-tagged) - Human 2-hydroxyacyl-CoA lyase 1 (HACL1) |
CNY 4,370.00 |
|
| SC127113 | HACL1 (untagged)-Human 2-hydroxyacyl-CoA lyase 1 (HACL1) |
CNY 2,950.00 |
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