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Antizyme inhibitor 1 (AZIN1) (NM_015878) Human Tagged ORF Clone
CAT＃: RC200022
AZIN1 (Myc-DDK-tagged)-Human antizyme inhibitor 1 (AZIN1), transcript variant 1
ORF Plasmid: tGFP
"NM_015878" in other vectors (7)
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CNY 5,320.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AZI; AZI1; AZIA1; OAZI; OAZIN; ODC1L |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200022 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGGATTTATTGATGATGCAAACTACTCCGTTGGCCTGTTGGATGAAGGAACAAACCTTGGAAATG TTATTGATAACTATGTTTATGAACATACCCTGACAGGGAAAAATGCATTTTTTGTGGGAGATCTTGGAAA GATTGTGAAGAAACACAGTCAATGGCAGAATGTAGTGGCTCAGATAAAGCCATTCTACACAGTGAAGTGC AACTCTGCTCCAGCTGTACTTGAGATTTTGGCAGCTCTTGGAACCGGATTTGCTTGTTCCAGTAAAAATG AAATGGCTTTAGTGCAAGAGTTGGGTGTACCTCCAGAAAACATTATTTACATAAGTCCTTGCAAGCAAGT GTCTCAGATAAAGTATGCAGCAAAAGTTGGAGTGAATATCCTGACATGTGACAATGAAATTGAATTGAAG AAAATTGCACGTAATCACCCAAATGCCAAGGTCTTACTACATATTGCAACAGAAGATAATATTGGAGGTG AAGAGGGTAACATGAAGTTTGGCACTACCCTGAAGAACTGTAGGCATCTCTTGGAATGTGCTAAGGAACT TGATGTCCAAATAATTGGGGTTAAATTTCATGTTTCGAGTGCTTGCAAAGAATCTCAAGTATATGTACAT GCTCTATCTGATGCTCGATGTGTGTTTGACATGGCTGGAGAAATTGGCTTTACGATGAACATGTTAGACA TTGGTGGAGGATTCACGGGAACTGAATTTCAATTGGAAGAGGTTAATCATGTTATCAGCCCTCTGTTGGA TATCTACTTTCCTGAAGGATCTGGTGTTAAGATAATTTCAGAACCCGGAAGCTACTATGTGTCTTCTGCA TTTACACTCGCAGTTAATATCATAGCAAAGAAAGTTGTTGAAAATGATAAATTTCCCTCTGGAGTAGAAA AAACCGGAAGTGATGAACCAGCCTTCATGTATTATATGAATGATGGTGTTTATGGTTCTTTTGCAAGTAA ACTGTCTGAGGACTTAAATACCATTCCAGAGGTTCACAAGAAATACAAGGAAGATGAGCCTCTGTTTACA AGCAGCCTTTGGGGTCCATCCTGTGATGAGCTTGATCAAATTGTGGAAAGCTGTCTTCTTCCTGAGCTGA ATGTGGGAGATTGGCTTATCTTTGATAACATGGGAGCAGATTCTTTCCATGAACCATCTGCTTTTAATGA TTTTCAGAGGCCAGCCATTTATTACATGATGTCATTCAGTGATTGGTATGAGATGCAAGATGCTGGAATT ACTTCAGACTCAATGATGAAGAACTTCTTCTTTGTGCCTTCTTGCATTCAGCTGAGCCAAGAAGACAGCT TTTCCGCTGAAGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200022 protein sequence
Red=Cloning site Green=Tags(s) MKGFIDDANYSVGLLDEGTNLGNVIDNYVYEHTLTGKNAFFVGDLGKIVKKHSQWQNVVAQIKPFYTVKC NSAPAVLEILAALGTGFACSSKNEMALVQELGVPPENIIYISPCKQVSQIKYAAKVGVNILTCDNEIELK KIARNHPNAKVLLHIATEDNIGGEEGNMKFGTTLKNCRHLLECAKELDVQIIGVKFHVSSACKESQVYVH ALSDARCVFDMAGEIGFTMNMLDIGGGFTGTEFQLEEVNHVISPLLDIYFPEGSGVKIISEPGSYYVSSA FTLAVNIIAKKVVENDKFPSGVEKTGSDEPAFMYYMNDGVYGSFASKLSEDLNTIPEVHKKYKEDEPLFT SSLWGPSCDELDQIVESCLLPELNVGDWLIFDNMGADSFHEPSAFNDFQRPAIYYMMSFSDWYEMQDAGI TSDSMMKNFFFVPSCIQLSQEDSFSAEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015878 |
| ORF Size | 1344 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015878.5 |
| RefSeq Size | 4385 bp |
| RefSeq ORF | 1347 bp |
| Locus ID | 51582 |
| UniProt ID | O14977 |
| Domains | Orn_Arg_deC_N |
| Protein Families | Druggable Genome |
| MW | 49.5 kDa |
| Gene Summary | The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 1, the first member of this gene family that is ubiquitously expressed, and is localized in the nucleus and cytoplasm. Overexpression of antizyme inhibitor 1 gene has been associated with increased proliferation, cellular transformation and tumorigenesis. Gene knockout studies showed that homozygous mutant mice lacking functional antizyme inhibitor 1 gene died at birth with abnormal liver morphology. RNA editing of this gene, predominantly in the liver tissue, has been linked to the progression of hepatocellular carcinoma. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200022L1 | Lenti ORF clone of Human antizyme inhibitor 1 (AZIN1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC200022L2 | Lenti ORF clone of Human antizyme inhibitor 1 (AZIN1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC200022L3 | Lenti ORF clone of Human antizyme inhibitor 1 (AZIN1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200022L4 | Lenti ORF clone of Human antizyme inhibitor 1 (AZIN1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG200022 | AZIN1 (tGFP-tagged) - Human antizyme inhibitor 1 (AZIN1), transcript variant 1 |
CNY 7,088.00 |
|
| SC125518 | AZIN1 (untagged)-Human antizyme inhibitor 1 (AZIN1), transcript variant 1 |
CNY 5,488.00 |
|
| SC324431 | AZIN1 (untagged)-Human antizyme inhibitor 1 (AZIN1), transcript variant 1 |
CNY 5,488.00 |
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