Gab1 (NM_001301298) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AA408973; AW107238
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230993 representing NM_001301298 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGCGGCGAAGTGGTTTGCTCGGGATGGCTCCGCAAGTCGCCCCCGGAGAAGAAGTTGAAGCGTT ATGCGTGGAAGAGAAGGTGGTTTGTGTTGCGCAGTGGCCGTTTGACTGGAGACCCGGATGTCCTGGAGTA TTACAAAAACGATCATGCCAAGAAGCCTATTCGGATTATTGATTTAAATTTATGTCAGCAAGTTGATGCT GGGTTGACATTCAACAAAAAGGAGTTTGAAAACAGCTATATCTTTGATATCAACACCATCGACCGGATTT TCTACTTGGTGGCAGATAGTGAGGAAGACATGAACAAGTGGGTCCGTTGTATCTGTGACATCTGTGGATT CAATCCCACAGAAGAAGATCCTGTGAAGCCGCTGACTGGCTCCTCACAAGCACCCGTCGATTCACCTTTC GCTATAAGTACAGCACCAGCCTCCAGTCAGATGGAAGCTTCTTCAGTCGCGCTACCTCCTCCTTACCAGG TCATCAGCCTTCCGCCACACCCAGACACCCTCGGCCTCCAGGACGATCCACAAGACTACCTCTTGCTGAT CAACTGTCAAAGCAAGAAGCCTGAACCTAACAGAACCCTCTTTGACTCTGCCAAGCCCACCTTTTCTGAG ACAGACTGCAATGACAACGTCCCTTCCCACCAGACTCCTGCTTCCTCCCAGAGCAAACACGGAATGAATG GCTTTTTCCAGCAACAAATGATGTATGACTGCCCACCGTCCCGGCTGACATCTGTCTCGGGAGAGTCCAG CCTCTATAACCTGCCCAGGAGCTATTCCCATGACGTGTTGCCAAAGGAATCCCCATCAAGCACGGAGGCC GACGGGGAGCTGTACACCTTTAACACCCCATCTGGGACTGCAGGTGTAGAAACGCAGATGAGACATGTAT CCATCAGTTACGACATTCCGCCAACACCTGGCAACACTTACCAGATCCCACGGACATTTCCAGAAAGCAC ACTGGGACAGTCATCAAAGCTGGACACCATTCCTGATATCCCCCCACCTCGGCCACCAAAGCCACATCCA ACTCATGACCGGTCTCCTGTGGAAACGTGTGGAGTCCCACGCACGGCCTCGGACACTGACAGCAGTTACT GTATCCCTCCTCCAGCAGGCATGACGCCCTCCCGGAGTAATACCATTTCCACCGTGGATTTGAACAAGTT GCGGAAAGATGCTAGTTCTCAAGATTGCTATGATATTCCACGGACCTTTCCGAGCGATAGATCTAGTTCC CTGGAAGGCTTCCATAGCCAGTATAAAATCAAAAGCGTGTTGACAGCGGGAGGTGTCTCGGGTGAAGAGC TGGATGAGAACTACGTTCCCATGAACCCCAACTCGCCACCTCGACAACATTCCGGCAGCTTTACCGAGCC AATCCAGGAGCCAAACTATGTGCCAATGACCCCAGGGACCTTTGACTTTTCTTCCTTTGGAATGCAAGTC CCTCCTCCTGCTCATATGGGCTTCAGGTCCAGCCCAAAGACCCCTCCCAGGAGGCCAGTTCCTGTTGCTG ACTGTGAACCACCCCCGGTGGATAGGAACCTCAAGCCAGACAGAAAAGGTCAGAGTCCTAAAATTTTAAG ACCCAAACCCCATGGTTTAGAGCGAACTGATTCACAAACCATAGGTGACTTTGCTACAAGAAGAAAGGTC AAGCCGGCACCTTTAGACATAAAACCTCTGTCAGAATGGGAAGAGCTGCAAGCCCCAGTCAGATCTCCCA TCACCAGGAGCTTCGCTCGGGACTCCTCTAGGTTTCCCATGTCCCCTCGGCCTGATTCTGTGCACAGTAC GACATCGAGCAGCGACTCTCATGACAGTGAAGAGAACTATGTCCCCATGAATCCAAATCTGTCTGGCGAA GACCCGAATCTCTTTGCCAGCAACAGCCTTGATGGGGGAAGCAGCCCGATGAATAAACCCAAAGGAGACA AACAAGTCGAATACCTGGATTTAGACCTAGATTCTGGGAAGTCCACGCCACCACGGAAGCAAAAGAGCAG TGGTTCTGGCAGCAGCATGGCAGACGAGAGGGTGGATTACGTTGTGGTGGACCAACAGAAGACTCTGGCC CTGAAGAGTACCAGAGAAGCTTGGACGGATGGGAGGCAGTCCACAGAGTCCGAGACACCCACCAAGAATG TGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230993 representing NM_001301298 Red=Cloning site Green=Tags(s) MSGGEVVCSGWLRKSPPEKKLKRYAWKRRWFVLRSGRLTGDPDVLEYYKNDHAKKPIRIIDLNLCQQVDA GLTFNKKEFENSYIFDINTIDRIFYLVADSEEDMNKWVRCICDICGFNPTEEDPVKPLTGSSQAPVDSPF AISTAPASSQMEASSVALPPPYQVISLPPHPDTLGLQDDPQDYLLLINCQSKKPEPNRTLFDSAKPTFSE TDCNDNVPSHQTPASSQSKHGMNGFFQQQMMYDCPPSRLTSVSGESSLYNLPRSYSHDVLPKESPSSTEA DGELYTFNTPSGTAGVETQMRHVSISYDIPPTPGNTYQIPRTFPESTLGQSSKLDTIPDIPPPRPPKPHP THDRSPVETCGVPRTASDTDSSYCIPPPAGMTPSRSNTISTVDLNKLRKDASSQDCYDIPRTFPSDRSSS LEGFHSQYKIKSVLTAGGVSGEELDENYVPMNPNSPPRQHSGSFTEPIQEPNYVPMTPGTFDFSSFGMQV PPPAHMGFRSSPKTPPRRPVPVADCEPPPVDRNLKPDRKGQSPKILRPKPHGLERTDSQTIGDFATRRKV KPAPLDIKPLSEWEELQAPVRSPITRSFARDSSRFPMSPRPDSVHSTTSSSDSHDSEENYVPMNPNLSGE DPNLFASNSLDGGSSPMNKPKGDKQVEYLDLDLDSGKSTPPRKQKSSGSGSSMADERVDYVVVDQQKTLA LKSTREAWTDGRQSTESETPTKNVK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001301298
ORF Size	2175 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301298.1, NP_001288227.1
RefSeq Size	5006 bp
RefSeq ORF	2178 bp
Locus ID	14388
MW	80.6 kDa
Gene Summary	Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway.[UniProtKB/Swiss-Prot Function]

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