Ecsit (NM_001253898) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Sitpec
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229893 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTGGGTGCAGGTCAACTTGCTGGTGCGAAGCCTCTCTAGGGGCTGGGGAGGCCTCTGCAGGCCTG CCCTTTCAGGAACTCCCTTTGCTCAGGTGTCCCTCCAGGCTCTACGAGGCCTTCACTGTAGTGCAGCTAC ACACAAGGATGAGCCGTGGTTGGTTCCTCGACCCCCGGAGCCCCAGAGGAAGCCCATCAAGGTTCCAGCA ATGCATGAAGACTCGTTCAAGCCATCAGGAAACAGGGAGCGGGACAAGGCAAGCTTCCTGAACGCAGTGC GCAGCTTTGGGGCGCACAATGTGCGCAAGCGCGGCCACGTGGACTTCATCTACCTAGCACTGCGCAAGAT GCCAGAGTTTGGTGTGGAGCGGGACTTGTCAGTATACAACCTGCTGCTGGATGTTTTCCCCAAGGAGGTC TTCCGGCCCCGCAACGTTATCCAGCGCATCTTCGTCCACTACCCACGGCAGCAGGAATGTGGGGTCGCAG TCCTGGAGCAGATGGAACGACACGGGGTCATGCCCAGCGCAGAGACAGAGTTCCTACTGATTCAGATATT CGGGCGCAAAAGTTACCCCATGCTCAAGTTCCTGCGGATGAAGCTGTGGTTCACCCGATTCAAGAATATC AACCCCTACCCAGTGCCCCGAGATCTTCCCCAGGACCCTTTGGACCTGGCCAAGCTAGGCCTGCGACACA TGGAGCCTGACCTCAGTGCTAAGGTCACTGTCTACCAGATGTCTTTGCCCAGTGACTCGACAGGCATGGA AGATCCCACACAGCCTCACATTGTAGGAATCCAGAGTCCAGATCAGCAAGCTGCCCTGGCCCGCCACAAC CCATCCAGGCCTGTTTTTGTTGAGGGCCCCTTCCCTCTCTGGCTTCGTAATAAGTGTGTCTACTATCACA TCCTAAGAGCTGACCTGCCACCTCCTGAGGAAGAGAAAGTAGAGGAGATCCCAGAAGAATGGGAGCTGTA CTACCCACAGAAGCTGGACCTGGAATATTCAAGGAGTGGTTGGGACGACTATGAGTTTGACGTGGATGAA GTGACAGAAGGCCCTGTCTTCGCCATGTGCATGGCTGGTGCCCATGATCAGGCAACATTGATCAAGTGGA TCCAAGGCTTGCAGGAGACCAACCCAACCCTGGCACAGATCCCAGTGGTATTCCGCCTGGCCAGGTCCAC GGGGGAGCTCCTGACAACTTCAAGGCTGGAGGGACAGTCCCCTCCCCACAGTCCTCCCAAGGGGCCTGAG GAAGATGATGAGACCATTCAGGCAGAGCAGCAGCAGGGGCAAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229893 protein sequence Red=Cloning site Green=Tags(s) MSWVQVNLLVRSLSRGWGGLCRPALSGTPFAQVSLQALRGLHCSAATHKDEPWLVPRPPEPQRKPIKVPA MHEDSFKPSGNRERDKASFLNAVRSFGAHNVRKRGHVDFIYLALRKMPEFGVERDLSVYNLLLDVFPKEV FRPRNVIQRIFVHYPRQQECGVAVLEQMERHGVMPSAETEFLLIQIFGRKSYPMLKFLRMKLWFTRFKNI NPYPVPRDLPQDPLDLAKLGLRHMEPDLSAKVTVYQMSLPSDSTGMEDPTQPHIVGIQSPDQQAALARHN PSRPVFVEGPFPLWLRNKCVYYHILRADLPPPEEEKVEEIPEEWELYYPQKLDLEYSRSGWDDYEFDVDE VTEGPVFAMCMAGAHDQATLIKWIQGLQETNPTLAQIPVVFRLARSTGELLTTSRLEGQSPPHSPPKGPE EDDETIQAEQQQGQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001253898
ORF Size	1305 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253898.1, NP_001240827.1
RefSeq Size	1496 bp
RefSeq ORF	1308 bp
Locus ID	26940
UniProt ID	Q9QZH6
MW	49.8 kDa
Gene Summary	Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.