Mavs (NM_001206382) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	cardif; D430028G21Rik; IPS-1; Visa
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229300 representing NM_001206382 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGTGGCTCTTTGATACCCTCTCCTAACCAGCAGGCTCTCAGCCCTCAGCCCTCCAGAGAGCATC AAGAGCAAGAACCAGAACTGGGTGGCGCCCACGCAGCAAATGTTGCCTCTGTTCCCATAGCAACCTATGG ACCTGTGTCTCCAACCGTTTCCTTCCAGCCCCTTCCACGTACTGCCCTGAGGACAAACCTCTTGTCTGGG GTCACAGTATCAGCCCTATCTGCTGATACCTCTTTGTCCTCCTCGTCCACTGGATCAGCTTTTGCAAAGG GAGCTGGTGACCAGGCCAAAGCTGCCACCTGTTTCAGTACTACACTCACCAATTCTGTGACTACCAGCTC AGTGCCTTCTCCCAGATTGGTCCCAGTAAAAACCATGTCTTCCAAGTTGCCCCTCAGTTCAAAGTCCACT GCTGCGATGACGTCTACTGTGCTCACCAATACAGCGCCATCAAAATTACCCAGCAACTCAGTGTATGCGG GCACAGTGCCATCCAGAGTGCCTGCTAGTGTGGCCAAAGCACCTGCCAACACAATACCACCTGAGAGGAA CAGCAAGCAAGCCAAGGAGACCCCGGAGGGTCCAGCAACCAAAGTCACCACTGGAGGCAACCAGACTGGA CCAAATAGCAGTATCAGGAGCTTGCACTCTGGACCAGAGATGAGCAAGCCAGGTGTGCTGGTATCCCAGT TGGACGAGCCATTCTCAGCCTGCTCTGTGGACCTTGCCATTAGCCCTAGCAGCTCCTTGGTCTCAGAACC CAACCATGGTCCAGAGGAGAATGAGTATTCGTCCTTTAGAATCCAGGTAGACGAAAGCCCCAGTGCTGAT CTATTAGGAAGCCCTGAGCCACTAGCCACCCAGCAGCCCCAAGAAGAGGAAGAACATTGTGCCAGTTCAA TGCCCTGGGCTAAGTGGCTTGGGGCCACCAGTGCACTCTTGGCTGTATTCCTGGCAGTGATGCTGTACCG TAGTAGGCGCCTGGCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229300 representing NM_001206382 Red=Cloning site Green=Tags(s) MSGGSLIPSPNQQALSPQPSREHQEQEPELGGAHAANVASVPIATYGPVSPTVSFQPLPRTALRTNLLSG VTVSALSADTSLSSSSTGSAFAKGAGDQAKAATCFSTTLTNSVTTSSVPSPRLVPVKTMSSKLPLSSKST AAMTSTVLTNTAPSKLPSNSVYAGTVPSRVPASVAKAPANTIPPERNSKQAKETPEGPATKVTTGGNQTG PNSSIRSLHSGPEMSKPGVLVSQLDEPFSACSVDLAISPSSSLVSEPNHGPEENEYSSFRIQVDESPSAD LLGSPEPLATQQPQEEEEHCASSMPWAKWLGATSALLAVFLAVMLYRSRRLAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001206382
ORF Size	999 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001206382.1, NP_001193311.1
RefSeq Size	2962 bp
RefSeq ORF	1002 bp
Locus ID	228607
MW	34.8 kDa
Gene Summary	Required for innate immune defense against viruses (PubMed:24037184). Acts downstream of DHX33, DDX58/RIG-I and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5) (PubMed:24037184). Peroxisomal and mitochondrial MAVS act sequentially to create an antiviral cellular state (By similarity). Upon viral infection, peroxisomal MAVS induces the rapid interferon-independent expression of defense factors that provide short-term protection, whereas mitochondrial MAVS activates an interferon-dependent signaling pathway with delayed kinetics, which amplifies and stabilizes the antiviral response (By similarity). May activate the same pathways following detection of extracellular dsRNA by TLR3 (By similarity). May protect cells from apoptosis (By similarity).[UniProtKB/Swiss-Prot Function]

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