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Cblb (NM_001033238) Mouse Tagged ORF Clone
CAT＃: MR227649
- TrueORF®
Cblb (Myc-DDK-tagged) - Mouse Casitas B-lineage lymphoma b (Cblb)
ORF Plasmid: tGFP
"NM_001033238" in other vectors (4)
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CNY 6,872.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI429560; AI851073; Cbl-b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227649 representing NM_001033238
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAATTCTATGAATGGCAGAAATCCTGGTGGTCGAGGAGGAAACCCCCGCAAAGGTCGCATTTTGG GGATTATTGATGCTATTCAGGATGCAGTCGGACCCCCAAAGCAAGCTGCAGCTGACCGCAGGACTGTGGA GAAGACTTGGAAACTCATGGACAAAGTGGTAAGACTGTGCCAAAATCCCAAGCTTCAGTTGAAAAACAGC CCACCGTATATACTTGATATTTTACCTGATACGTATCAGCACTTGAGACTTATATTGAGTAAATATGATG ACAACCAGAAGCTGGCTCAACTGAGCGAGAATGAGTACTTTAAAATCTACATCGATAGTCTCATGAAGAA GTCGAAGCGAGCGATCCGGCTCTTTAAAGAAGGCAAGGAAAGGATGTACGAAGAGCAGTCGCAGGACAGA CGGAATCTCACAAAGCTGTCCCTTATCTTCAGTCACATGCTGGCAGAAATCAAGGCGATCTTTCCCAATG GCCAGTTCCAGGGAGATAACTTCCGGATCACCAAAGCAGATGCTGCTGAGTTCTGGAGGAAGTTTTTTGG AGACAAAACTATTGTACCATGGAAAGTCTTCAGACAGTGCCTGCATGAGGTCCATCAGATCAGCTCTGGC CTGGAAGCAATGGCTCTGAAGTCAACCATTGATTTAACTTGCAATGATTACATCTCAGTGTTTGAATTTG ATATTTTTACCAGGCTATTTCAGCCCTGGGGCTCTATTTTACGGAATTGGAACTTCTTGGCTGTGACTCA CCCGGGATACATGGCATTTCTCACGTATGATGAAGTTAAAGCTCGGCTACAGAAATACAGCACCAAGCCT GGAAGTTACATTTTCCGGTTAAGCTGCACTCGGCTGGGACAATGGGCCATTGGCTATGTGACTGGGGACG GCAATATCCTACAGACCATACCTCATAACAAGCCCCTGTTCCAAGCCCTGATTGATGGTAGCAGGGAAGG CTTTTATCTTTATCCTGATGGGCGGAGCTATAACCCTGATTTAACCGGATTATGTGAACCTACACCTCAC GATCATATAAAAGTTACACAGGAGCAGTATGAACTGTATTGTGAAATGGGCTCCACTTTTCAGCTCTGCA AGATCTGTGCAGAGAATGACAAAGATGTCAAGATTGAGCCTTGCGGGCATCTGATGTGCACTTCGTGCCT TACCGCGTGGCAGGAGTCTGATGGCCAAGGCTGCCCCTTCTGTCGCTGTGAGATAAAAGGAACGGAGCCC ATCATTGTGGACCCCTTCGACCCCAGAGATGAAGGCTCCAGGTGCTGCAGCATCATTGACCCTTTCAGCA TCCCCATGCTTGACTTGGACGATGACGATGATCGAGAGGAGTCTTTGATGATGAACAGGCTGGCGAGTGT TCGAAAGTGCACGGACAGGCAGAACTCACCAGTCACATCGCCAGGCTCCTCACCCCTTGCCCAGAGAAGA AAGCCTCAGCCAGACCCTCTCCAGATCCCCCACCTCAGCCTGCCACCAGTGCCTCCCCGCCTAGATCTCA TTCAGAAAGGCATCGTGCGCTCTCCGTGTGGCAGCCCCACAGGCTCGCCAAAGTCTTCTCCATGCATGGT TAGAAAACAAGACAAACCACTCCCGGCACCCCCTCCTCCCTTGAGAGATCCACCTCCTCCACCAGAGAGG CCTCCCCCCATCCCACCTGACAATAGACTGAGCAGACACTTCCACCATGGAGAGAGTGTGCCTTCCCGGG ACCAGCCCATGCCCCTCGAAGCCTGGTGCCCTCGGGATGCCTTCGGGACTAACCAGGTGATGGGATGTCG CATCCTCGGGGATGGCTCTCCAAAGCCTGGCGTCACCGCAAACTCCAGCTTAAATGGGAGGCACAGTAGA ATGGGCTCCGAGCAGGTTCTTATGAGGAAACACAGACGCCATGATTTGCCTTCAGAAGGAGCCAAGGTCT TTTCCAATGGCCACCTTGCCACTGAAGAATACGATGTCCCTCCTCGGCTTTCCCCTCCGCCTCCAGTCAC CACCCTTCTCCCAAGCATAAAGTGTACTGGTCCATTAGCAAATTGTCTCTCAGAGAAAACTAGAGACACG GTAGAAGATGATGACGATGAATACAAGATTCCTTCATCCCATCCTGTGTCCCTGAATTCACAACCATCTC ATTGCCATAATGTCAAAGCTCCTGTTCGGTCTTGTGATAATGGTCATTGTATATTGAATGGAACTCATGG TGCGCCTTCAGAGATGAAGAAATCGAACATCCCAGATTTAGGCATCTATTTGAAGGGTGAGGACGCCTTT GATGCTCTCCCTCCATCCCTCCCTCCTCCCCCACCTCCTGCAAGACACAGTCTCATCGAACATTCAAAAC CTCCAGGCTCCAGTAGCCGGCCTTCCTCAGGGCAGGACCTTTTCCTTCTTCCTTCAGATCCCTTTTTTGA CCCAACAAGTGGCCAAGTTCCATTGCCTCCTGCCAGGAGAGCAGCAGGAGACAGCGGCAAAGCCAACAGA GCCTCGCAGGACTATGACCAGCTCCCTTCATCTTCCGATGGTTCGCAAGCACCAGCTAGACCCCCCAAAC CACGACCCCGAAGGACTGCACCAGAAATTCATCACAGAAAGCCCCATGGGCCCGAAGCGGCGTTGGAAAA TGTCGATGCAAAAATTGCAAAACTCATGGGAGAGGGGTATGCCTTTGAAGAGGTGAAGAGAGCCTTAGAG ATTGCCCAGAATAACGTGGAAGTGGCCAGGAGCATACTTCGAGAATTTGCCTTCCCCCCTCCTGTCTCCC CACGTCTGAATCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227649 representing NM_001033238
Red=Cloning site Green=Tags(s) MANSMNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKNS PPYILDILPDTYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDR RNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQISSG LEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKYSTKP GSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDGRSYNPDLTGLCEPTPH DHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESDGQGCPFCRCEIKGTEP IIVDPFDPRDEGSRCCSIIDPFSIPMLDLDDDDDREESLMMNRLASVRKCTDRQNSPVTSPGSSPLAQRR KPQPDPLQIPHLSLPPVPPRLDLIQKGIVRSPCGSPTGSPKSSPCMVRKQDKPLPAPPPPLRDPPPPPER PPPIPPDNRLSRHFHHGESVPSRDQPMPLEAWCPRDAFGTNQVMGCRILGDGSPKPGVTANSSLNGRHSR MGSEQVLMRKHRRHDLPSEGAKVFSNGHLATEEYDVPPRLSPPPPVTTLLPSIKCTGPLANCLSEKTRDT VEDDDDEYKIPSSHPVSLNSQPSHCHNVKAPVRSCDNGHCILNGTHGAPSEMKKSNIPDLGIYLKGEDAF DALPPSLPPPPPPARHSLIEHSKPPGSSSRPSSGQDLFLLPSDPFFDPTSGQVPLPPARRAAGDSGKANR ASQDYDQLPSSSDGSQAPARPPKPRPRRTAPEIHHRKPHGPEAALENVDAKIAKLMGEGYAFEEVKRALE IAQNNVEVARSILREFAFPPPVSPRLNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033238 |
| ORF Size | 2814 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033238.1, NP_001028410.1 |
| RefSeq Size | 6323 bp |
| RefSeq ORF | 2817 bp |
| Locus ID | 208650 |
| UniProt ID | Q3TTA7 |
| MW | 105 kDa |
| Gene Summary | E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBL, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA (PubMed:29237719).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222751 | Cblb (untagged) - Mouse Casitas B-lineage lymphoma b (Cblb), (10ug) |
CNY 12,070.00 |
|
| MG227649 | Cblb (tGFP-tagged) - Mouse Casitas B-lineage lymphoma b (Cblb), (10ug) |
CNY 7,890.00 |
|
| MR227649L3 | Lenti ORF clone of Cblb (Myc-DDK-tagged) - Mouse Casitas B-lineage lymphoma b (Cblb) |
CNY 9,030.00 |
|
| MR227649L4 | Lenti ORF clone of Cblb (mGFP-tagged) - Mouse Casitas B-lineage lymphoma b (Cblb) |
CNY 9,030.00 |
