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Tert (NM_009354) Mouse Tagged ORF Clone
CAT＃: MR226892
- TrueORF®
Tert (Myc-DDK-tagged) - Mouse telomerase reverse transcriptase (Tert)
ORF Plasmid: tGFP
"NM_009354" in other vectors (4)
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CNY 11,720.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EST2; TCS1; TP2; TR; TRT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226892 representing NM_009354
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCGCGCTCCTCGTTGCCCCGCGGTGCGCTCTCTGCTGCGCAGCCGATACCGGGAGGTGTGGCCGC TGGCAACCTTTGTGCGGCGCCTGGGGCCCGAGGGCAGGCGGCTTGTGCAACCCGGGGACCCGAAGATCTA CCGCACTTTGGTTGCCCAATGCCTAGTGTGCATGCACTGGGGCTCACAGCCTCCACCTGCCGACCTTTCC TTCCACCAGGTGTCATCCCTGAAAGAGCTGGTGGCCAGGGTTGTGCAGAGACTCTGCGAGCGCAACGAGA GAAACGTGCTGGCTTTTGGCTTTGAGCTGCTTAACGAGGCCAGAGGCGGGCCTCCCATGGCCTTCACTAG TAGCGTGCGTAGCTACTTGCCCAACACTGTTATTGAGACCCTGCGTGTCAGTGGTGCATGGATGCTACTG TTGAGCCGAGTGGGCGACGACCTGCTGGTCTACCTGCTGGCACACTGTGCTCTTTATCTTCTGGTGCCCC CCAGCTGTGCCTACCAGGTGTGTGGGTCTCCCCTGTACCAAATTTGTGCCACCACGGATATCTGGCCCTC TGTGTCCGCTAGTTACAGGCCCACCCGACCCGTGGGCAGGAATTTCACTAACCTTAGGTTCTTACAACAG ATCAAGAGCAGTAGTCGCCAGGAAGCACCGAAACCCCTGGCCTTGCCATCTCGAGGTACAAAGAGGCATC TGAGTCTCACCAGTACAAGTGTGCCTTCAGCTAAGAAGGCCAGATGCTATCCTGTCCCGAGAGTGGAGGA GGGACCCCACAGGCAGGTGCTACCAACCCCATCAGGCAAATCATGGGTGCCAAGTCCTGCTCGGTCCCCC GAGGTGCCTACTGCAGAGAAAGATTTGTCTTCTAAAGGAAAGGTGTCTGACCTGAGTCTCTCTGGGTCGG TGTGCTGTAAACACAAGCCCAGCTCCACATCTCTGCTGTCACCACCCCGCCAAAATGCCTTTCAGCTCAG GCCATTTATTGAGACCAGACATTTCCTTTACTCCAGGGGAGATGGCCAAGAGCGTCTAAACCCCTCATTC CTACTCAGCAACCTCCAGCCTAACTTGACTGGGGCCAGGAGACTGGTGGAGATCATCTTTCTGGGCTCAA GGCCTAGGACATCAGGACCACTCTGCAGGACACACCGTCTATCGCGTCGATACTGGCAGATGCGGCCCCT GTTCCAACAGCTGCTGGTGAACCATGCAGAGTGCCAATATGTCAGACTCCTCAGGTCACATTGCAGGTTT CGAACAGCAAACCAACAGGTGACAGATGCCTTGAACACCAGCCCACCGCACCTCATGGATTTGCTCCGCC TGCACAGCAGTCCCTGGCAGGTATATGGTTTTCTTCGGGCCTGTCTCTGCAAGGTGGTGTCTGCTAGTCT CTGGGGTACCAGGCACAATGAGCGCCGCTTCTTTAAGAACTTAAAGAAGTTCATCTCGTTGGGGAAATAC GGCAAGCTATCACTGCAGGAACTGATGTGGAAGATGAAAGTAGAGGATTGCCACTGGCTCCGCAGCAGCC CGGGGAAGGACCGTGTCCCCGCTGCAGAGCACCGTCTGAGGGAGAGGATCCTGGCTACGTTCCTGTTCTG GCTGATGGACACATACGTGGTACAGCTGCTTAGGTCATTCTTTTACATCACAGAGAGCACATTCCAGAAG AACAGGCTCTTCTTCTACCGTAAGAGTGTGTGGAGCAAGCTGCAGAGCATTGGAGTCAGGCAACACCTTG AGAGAGTGCGGCTACGGGAGCTGTCACAAGAGGAGGTCAGGCATCACCAGGACACCTGGCTAGCCATGCC CATCTGCAGACTGCGCTTCATCCCCAAGCCCAACGGCCTGCGGCCCATTGTGAACATGAGTTATAGCATG GGTACCAGAGCTTTGGGCAGAAGGAAGCAGGCCCAGCATTTCACCCAGCGTCTCAAGACTCTCTTCAGCA TGCTCAACTATGAGCGGACAAAACATCCTCACCTTATGGGGTCTTCTGTACTGGGTATGAATGACATCTA CAGGACCTGGCGGGCCTTTGTGCTGCGTGTGCGTGCTCTGGACCAGACACCCAGGATGTACTTTGTTAAG GCAGATGTGACCGGGGCCTATGATGCCATCCCCCAGGGTAAGCTGGTGGAGGTTGTTGCCAATATGATCA GGCACTCGGAGAGCACGTACTGTATCCGCCAGTATGCAGTGGTCCGGAGAGATAGCCAAGGCCAAGTCCA CAAGTCCTTTAGGAGACAGGTCACCACCCTCTCTGACCTCCAGCCATACATGGGCCAGTTCCTTAAGCAT CTGCAGGATTCAGATGCCAGTGCACTGAGGAACTCCGTTGTCATCGAGCAGAGCATCTCTATGAATGAGA GCAGCAGCAGCCTGTTTGACTTCTTCCTGCACTTCCTGCGTCACAGTGTCGTAAAGATTGGTGACAGGTG CTATACGCAGTGCCAGGGCATCCCCCAGGGCTCCAGCCTATCCACCCTGCTCTGCAGTCTGTGTTTCGGA GACATGGAGAACAAGCTGTTTGCTGAGGTGCAGCGGGATGGGTTGCTTTTACGTTTTGTTGATGACTTTC TGTTGGTGACGCCTCACTTGGACCAAGCAAAAACCTTCCTCAGCACCCTGGTCCATGGCGTTCCTGAGTA TGGGTGCATGATAAACTTGCAGAAGACAGTGGTGAACTTCCCTGTGGAGCCTGGTACCCTGGGTGGTGCA GCTCCATACCAGCTGCCTGCTCACTGCCTGTTTCCCTGGTGTGGCTTGCTGCTGGACACTCAGACTTTGG AGGTGTTCTGTGACTACTCAGGTTATGCCCAGACCTCAATTAAGACGAGCCTCACCTTCCAGAGTGTCTT CAAAGCTGGGAAGACCATGCGGAACAAGCTCCTGTCGGTCTTGCGGTTGAAGTGTCACGGTCTATTTCTA GACTTGCAGGTGAACAGCCTCCAGACAGTCTGCATCAATATATACAAGATCTTCCTGCTTCAGGCCTACA GGTTCCATGCATGTGTGATTCAGCTTCCCTTTGACCAGCGTGTTAGGAAGAACCTCACATTCTTTCTGGG CATCATCTCCAGCCAAGCATCCTGCTGCTATGCTATCCTGAAGGTCAAGAATCCAGGAATGACACTAAAG GCCTCTGGCTCCTTTCCTCCTGAAGCCGCACATTGGCTCTGCTACCAGGCCTTCCTGCTCAAGCTGGCTG CTCATTCTGTCATCTACAAATGTCTCCTGGGACCTCTGAGGACAGCCCAAAAACTGCTGTGCCGGAAGCT CCCAGAGGCGACAATGACCATCCTTAAAGCTGCAGCTGACCCAGCCCTAAGCACAGACTTTCAGACCATT TTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226892 representing NM_009354
Red=Cloning site Green=Tags(s) MTRAPRCPAVRSLLRSRYREVWPLATFVRRLGPEGRRLVQPGDPKIYRTLVAQCLVCMHWGSQPPPADLS FHQVSSLKELVARVVQRLCERNERNVLAFGFELLNEARGGPPMAFTSSVRSYLPNTVIETLRVSGAWMLL LSRVGDDLLVYLLAHCALYLLVPPSCAYQVCGSPLYQICATTDIWPSVSASYRPTRPVGRNFTNLRFLQQ IKSSSRQEAPKPLALPSRGTKRHLSLTSTSVPSAKKARCYPVPRVEEGPHRQVLPTPSGKSWVPSPARSP EVPTAEKDLSSKGKVSDLSLSGSVCCKHKPSSTSLLSPPRQNAFQLRPFIETRHFLYSRGDGQERLNPSF LLSNLQPNLTGARRLVEIIFLGSRPRTSGPLCRTHRLSRRYWQMRPLFQQLLVNHAECQYVRLLRSHCRF RTANQQVTDALNTSPPHLMDLLRLHSSPWQVYGFLRACLCKVVSASLWGTRHNERRFFKNLKKFISLGKY GKLSLQELMWKMKVEDCHWLRSSPGKDRVPAAEHRLRERILATFLFWLMDTYVVQLLRSFFYITESTFQK NRLFFYRKSVWSKLQSIGVRQHLERVRLRELSQEEVRHHQDTWLAMPICRLRFIPKPNGLRPIVNMSYSM GTRALGRRKQAQHFTQRLKTLFSMLNYERTKHPHLMGSSVLGMNDIYRTWRAFVLRVRALDQTPRMYFVK ADVTGAYDAIPQGKLVEVVANMIRHSESTYCIRQYAVVRRDSQGQVHKSFRRQVTTLSDLQPYMGQFLKH LQDSDASALRNSVVIEQSISMNESSSSLFDFFLHFLRHSVVKIGDRCYTQCQGIPQGSSLSTLLCSLCFG DMENKLFAEVQRDGLLLRFVDDFLLVTPHLDQAKTFLSTLVHGVPEYGCMINLQKTVVNFPVEPGTLGGA APYQLPAHCLFPWCGLLLDTQTLEVFCDYSGYAQTSIKTSLTFQSVFKAGKTMRNKLLSVLRLKCHGLFL DLQVNSLQTVCINIYKIFLLQAYRFHACVIQLPFDQRVRKNLTFFLGIISSQASCCYAILKVKNPGMTLK ASGSFPPEAAHWLCYQAFLLKLAAHSVIYKCLLGPLRTAQKLLCRKLPEATMTILKAAADPALSTDFQTI LD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009354 |
| ORF Size | 3366 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009354.2 |
| RefSeq Size | 3426 bp |
| RefSeq ORF | 3369 bp |
| Locus ID | 21752 |
| UniProt ID | O70372 |
| MW | 128.4 kDa |
| Gene Summary | Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223645 | Tert (untagged) - Mouse telomerase reverse transcriptase (Tert), (10ug) |
CNY 11,728.00 |
|
| MG226892 | Tert (tGFP-tagged) - Mouse telomerase reverse transcriptase (Tert), (10ug) |
CNY 13,320.00 |
|
| MR226892L3 | Lenti ORF clone of Tert (Myc-DDK-tagged) - Mouse telomerase reverse transcriptase (Tert) |
CNY 14,120.00 |
|
| MR226892L4 | Lenti ORF clone of Tert (mGFP-tagged) - Mouse telomerase reverse transcriptase (Tert) |
CNY 14,120.00 |
