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Rasgrf2 (NM_009027) Mouse Tagged ORF Clone
CAT＃: MR226568
- TrueORF®
Rasgrf2 (Myc-DDK-tagged) - Mouse RAS protein-specific guanine nucleotide-releasing factor 2 (Rasgrf2)
ORF Plasmid: tGFP
"NM_009027" in other vectors (4)
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CNY 8,280.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6330417G04Rik; AW048350; Grf2; Ras-GRF2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226568 representing NM_009027
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAAGAGCGTGCGCTACAACGAGGGGCACGCCCTGTACCTGGCCATGCTGGCGCGCAAGGAGGGCA CCAAACGCGGCTTCCTGAGCAAGAAGGCTGCAGAGGCGAGCCGCTGGCACGAGAAGTGGTTCGCTCTCTA CCAGAATGTGCTCTTTTACTTCGAAGGCGAGCAGAGCGGCCGGCCAGCGGGCATGTACCTTCTAGAGGGC TGCAGCTGTGAGCGGACGCCTGCACCACCCAGGACCAACGCAGGACCCGCAGGCGCCCGGGACGCTTTGG ACAAGCAGTATTACTTCACTGTCCTTTTTGGGCACGATGGTCAAAAACCACTGGAGCTGCGCTGTGAGGA GGAGCAGGCCGGGAAAGAGTGGATGGAGGCCATTCACCAAGCCAGTTACGCGGACATTTTGATCGAGAGG GAAGTGCTAATGCAGAAGTACATTCATCTAGTTCAGATTGTGGAGACCGAGAAGATCGCCACCAACCAAC TCCGACATCAACTCGAAGATCAAGATACCGAAATCGAAAGGCTTAAATCAGAGATTGTTGCTCTTAATAA GACCAAGGAACGGATGCGTCCGTACCACGTCCATCAAGAGGAGGAGGATCCAGACATCAAGAAGATTAAG AAGGTTCAGAGCTTCATGAGAGGGTGGCTGTGTAGGAGGAAGTGGAAGACCATCGTGCAGGACTACATTT GTTCTCCCCATGCAGAGAGCATGAGGAAGAGAAACCAGATTGTGTTCACGATGGTGGAGGCCGAGACGGA GTATGTACACCAGCTTTACATCCTGGTGAACGGTTTCCTCAGGCCCCTGCGCATGGCAGCCAGCTCCAAG AAGCCCCCCATTAACCACGATGACGTCAGCAGCATTTTTCTCAACAGTGAAACAATCATGTTCCTTCATG AAATATTCCATCAAGGACTAAAGGCAAGGCTGGCAAATTGGCCTACTTTGGTTTTAGCGGATCTATTTGA TATTTTGCTGCCCATGCTGAACATTTATCAAGAATTTGTACGTAATCACCAGTACAGCCTCCAAGTACTT GCCAACTGTAAGCAAAACAGAGATTTTGACAAGCTCTTAAAGCAGTATGAAGCCAACCCTGCCTGTGAGG GGAGGATGCTGGAGACGTTCTTGACCTACCCAATGTTTCAGATTCCCAGGTATATCATCACGCTGCACGA ACTCCTGGCCCACACCCCCCATGAACATGTGGAGAGGAAAAGTCTGGAGTTCGCTAAGTCCAAGCTGGAG GAACTGTCCAGAGTGATGCATGATGAAGTCAGTGACACCGAGAACATACGGAAAAATCTCGCCATTGAGA GGATGATCGTAGAGGGCTGTGACATTTTGCTGGACACCAGCCAAACTTTTATCCGACAAGGTTCCCTTAT TCAAGTGCCTTCTGTGGAGAGGGGGAAACTGAGTAAAGTCCGCCTGGGCTCGCTGTCCCTGAAAAAGGAA GGCGAGAGACAGTGTTTCTTATTCACGAAGCACTTTCTGATTTGTACACGAAGTTCAGGAGGAAAGCTGC ATTTGCTCAAGACAGGTGGGGTTCTCTCCCTCATTCAGTGTACATTGATTGAGGAGCCCGATGGAAGTGA CGATGACCCCAAAGGTTCTGGCCACATGTTTGGACATCTGGATTTTAAAATCGTAGTGGAGCCTCCTGAC GCTGCCTCTTTCACTGTGGTCTTATTGGCACCTTCACGCCAGGAGAAGGCTGCATGGATGAGCGACATCA GTCAGTGTGTGGACAATATACGATGTAACGGATTGATGACTATAGTCTTTGAAGAGAATTCCAAAGTTAC TGTGCCACATATGATTAAGTCTGATGCCCGTCTTCACAAAGACGACACTGACATCTGTTTCAGTAAAACA CTCAATTCCTGCAAAGTGCCCCAGATCCGCTACGCCAGCGTGGAGCGCCTCTTGGAGCGGTTGACGGACT TGCGGTTTCTGAGCATTGATTTCCTCAACACCTTCCTGCACACCTACCGCATTTTCACCACGGCCACCGT AGTGCTGGCAAAGCTCTCAGACATCTACAAGAGGCCTTTCACCTCCATTCCTGTCAGGTCATTAGAGTTA TTTTTTGCCACAAGCCAGAACAACAGAGAACATTTGGTAGATGGAAAATCCCCACGTCTCTGTCGCAAGT TCTCTTCCCCACCTCCCCTGGCTGTGTCCAGAACATCCTCCCCAGTGAGGGCCAGAAAGCTGTCTTTGAC GTCTTCCTTGAACTCAAGGATTGGAGCCTTGGACCTGACTAATTCCTCATCTTCCAGTAGTCCCACCACC ACCACCCACAGCCCTGCTGCATCCCCACCACCGCACACTGCTGTCTTGGAGTCTGCACCAGCCGACAAAG CGGGAGATTCTGCAGACATGTCACCATGTAGATCCCCCACAACTCCCCGGCACCTCCGCTATCGGCAGCC TGGAGGACAGGTAGCCGACAGCGCCCACTGTTCTGTGTCACCTGCTTCTGCTTTTGCAATCGCCACAGCT GCAGCAGGACATGGGAGTCCACCAGGATTTAACAACGAGAGAACATGTGACAAGGAGTTCATCATACGGA GAACAGCCACCAATCGAGTCCTGAATGTCCTCCGCCACTGGGTCTCAAAGCATGCCCAGGATTTTGAACT CAATAATGAACTCAAAATGAATGTTCTAAACTTGCTAGAAGAAGTTCTACGAGACCCGGACCTTCTTCCT CAAGAAAGGAAAGCAACGGCGAACATCCTGAGGGCTCTTTCACAAGATGATCAAGATGATATCCACCTAA AATTAGAGGATATAATTCAAATGACAGACTGTCCAAAGGCCGAGTGCTTTGAGACGTTGTCAGCCATGGA GCTGGCCGAGCAGATCACTCTCCTGGACCACATTGTTTTCAGAAGCATCCCCTACGAAGAGTTTCTTGGG CAGGGCTGGATGAAGCTTGACAAGAACGAAAGGACGCCTTACATCATGAAAACCAGCCAGCACTTCAATG AGATGAGTAACCTGGTGGCCTCCCAGATAATGAATTATGCCGACATCAGCTCCCGTGCCAACGCCATTGA GAAGTGGGTGGCAGTGGCGGACATTTGCCGATGTCTCCACAACTACAATGGTGTTCTGGAGATCACCTCA GCCTTAAACAGAAGTGCCATCTACAGGCTGAAGAAAACCTGGGCCAAGGTGTCCAAGCAGACAAAAGCTC TAATGGACAAACTTCAGAAGACTGTTTCATCTGAAGGAAGATTTAAAAACCTCAGAGAGACTCTCAAAAA CTGTAACCCCCCAGCTGTCCCTTACCTTGGGATGTACTTGACAGATCTGGCATTCATTGAAGAAGGGACA CCGAACTTTACCGAAGAGGGCCTTGTCAATTTCTCCAAAATGCGAATGATATCACACATTATTCGAGAGA TACGCCAGTTCCAGCAGACCGCTTACCGCATAGACCAGCAGCCAAAGGTCATACAATACCTGCTTGACAA AGCCCTTGTCATAGACGAAGATTCACTCTATGAGCTGTCACTCAAAATTGAACCTCGACTCCCTGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226568 representing NM_009027
Red=Cloning site Green=Tags(s) MQKSVRYNEGHALYLAMLARKEGTKRGFLSKKAAEASRWHEKWFALYQNVLFYFEGEQSGRPAGMYLLEG CSCERTPAPPRTNAGPAGARDALDKQYYFTVLFGHDGQKPLELRCEEEQAGKEWMEAIHQASYADILIER EVLMQKYIHLVQIVETEKIATNQLRHQLEDQDTEIERLKSEIVALNKTKERMRPYHVHQEEEDPDIKKIK KVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAETEYVHQLYILVNGFLRPLRMAASSK KPPINHDDVSSIFLNSETIMFLHEIFHQGLKARLANWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQVL ANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLE ELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKE GERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIQCTLIEEPDGSDDDPKGSGHMFGHLDFKIVVEPPD AASFTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKT LNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTATVVLAKLSDIYKRPFTSIPVRSLEL FFATSQNNREHLVDGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSSLNSRIGALDLTNSSSSSSPTT TTHSPAASPPPHTAVLESAPADKAGDSADMSPCRSPTTPRHLRYRQPGGQVADSAHCSVSPASAFAIATA AAGHGSPPGFNNERTCDKEFIIRRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLP QERKATANILRALSQDDQDDIHLKLEDIIQMTDCPKAECFETLSAMELAEQITLLDHIVFRSIPYEEFLG QGWMKLDKNERTPYIMKTSQHFNEMSNLVASQIMNYADISSRANAIEKWVAVADICRCLHNYNGVLEITS ALNRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGT PNFTEEGLVNFSKMRMISHIIREIRQFQQTAYRIDQQPKVIQYLLDKALVIDEDSLYELSLKIEPRLPA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009027 |
| ORF Size | 3567 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009027.4 |
| RefSeq Size | 7595 bp |
| RefSeq ORF | 3570 bp |
| Locus ID | 19418 |
| UniProt ID | P70392 |
| MW | 136.1 kDa |
| Gene Summary | Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP. Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation. Functions in synaptic plasticity by contributing to the induction of long term potentiation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223885 | Rasgrf2 (untagged) - Mouse RAS protein-specific guanine nucleotide-releasing factor 2 (Rasgrf2), (10ug) |
CNY 15,300.00 |
|
| MG226568 | Rasgrf2 (tGFP-tagged) - Mouse RAS protein-specific guanine nucleotide-releasing factor 2 (Rasgrf2), (10ug) |
CNY 11,500.00 |
|
| MR226568L3 | Lenti ORF clone of Rasgrf2 (Myc-DDK-tagged) - Mouse RAS protein-specific guanine nucleotide-releasing factor 2 (Rasgrf2) |
CNY 12,350.00 |
|
| MR226568L4 | Lenti ORF clone of Rasgrf2 (mGFP-tagged) - Mouse RAS protein-specific guanine nucleotide-releasing factor 2 (Rasgrf2) |
CNY 12,350.00 |
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