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Gaa (NM_008064) Mouse Tagged ORF Clone
CAT＃: MR226319
- TrueORF®
Gaa (Myc-DDK-tagged) - Mouse glucosidase, alpha, acid (Gaa), transcript variant 1
ORF Plasmid: tGFP
"NM_008064" in other vectors (5)
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CNY 10,464.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | E430018M07Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226319 representing NM_008064
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATATACGGAAGCCCCTCTGTTCGAACTCCGTGGTTGGGGCCTGCACCCTTATCTCTCTGACTACAG CGGTCATCCTGGGTCATCTCATGCTTCGGGAGTTAATGCTGCTTCCCCAAGACCTTCATGAGTCCTCTTC AGGACTGTGGAAGACGTACCGACCTCACCACCAGGAAGGTTACAAGCCAGGGCCTCTGCACATCCAGGAG CAGACTGAACAGCCCAAAGAAGCACCCACACAGTGTGATGTGCCCCCCAGCAGCCGCTTTGACTGTGCCC CCGACAAAGGCATCTCACAGGAGCAATGCGAGGCCCGCGGCTGCTGCTATGTCCCAGCAGGGCAGGTGCT GAAGGAGCCGCAGATAGGGCAGCCCTGGTGTTTCTTCCCTCCCAGCTACCCAAGCTACCGTCTAGAGAAC CTGAGCTCTACAGAGTCGGGGTACACAGCCACCCTGACCCGTACCAGCCCGACCTTCTTCCCAAAGGATG TGCTGACCTTACAGCTGGAGGTGCTGATGGAGACAGACAGCCGCCTCCACTTCAAGATCAAAGATCCTGC TAGTAAGCGCTACGAAGTGCCCCTGGAGACCCCACGTGTGCTGAGCCAGGCACCATCCCCACTTTACAGC GTGGAATTCTCAGAGGAACCCTTTGGAGTGATCGTTCGTAGGAAGCTTGGTGGCCGAGTGTTGCTGAACA CAACCGTGGCCCCCCTGTTCTTCGCTGACCAGTTCCTGCAGCTGTCCACTTCCCTGCCCTCCCAGCACAT CACAGGCCTGGGGGAACACCTCAGCCCACTCATGCTCAGCACCGACTGGGCTCGTATCACCCTCTGGAAC CGGGACACACCACCCTCGCAAGGTACCAACCTCTACGGGTCACATCCTTTCTACCTGGCACTGGAGGACG GTGGCTTGGCTCACGGTGTCTTCTTGCTAAACAGCAATGCCATGGATGTCATCCTGCAACCCAGCCCAGC CCTAACCTGGAGGTCAACGGGCGGGATCCTGGATGTGTATGTGTTCCTAGGCCCAGAGCCCAAGAGCGTT GTGCAACAATACCTGGATGTTGTGGGATACCCCTTCATGCCTCCATACTGGGGCCTCGGCTTCCACCTCT GCCGCTGGGGCTACTCCTCGACCGCCATTGTCCGCCAGGTAGTGGAGAACATGACCAGGACACACTTCCC GCTGGATGTGCAATGGAATGACCTGGACTACATGGACGCCCGAAGAGACTTCACCTTCAACCAGGACAGC TTTGCCGACTTCCCAGACATGGTGCGGGAGCTGCACCAGGGTGGCCGGCGCTACATGATGATCGTGGACC CTGCCATCAGCAGCGCAGGCCCTGCTGGGAGTTACAGGCCCTACGACGAGGGTCTGCGGAGGGGTGTGTT CATCACCAACGAGACTGGGCAGCCGCTGATTGGGAAGGTTTGGCCCGGAACCACCGCCTTCCCTGATTTC ACCAACCCTGAGACCCTTGACTGGTGGCAGGACATGGTGTCTGAGTTCCACGCCCAGGTGCCCTTCGATG GCATGTGGCTCGACATGAACGAACCGTCCAACTTCGTTAGAGGCTCTCAGCAGGGCTGCCCCAACAATGA ACTGGAGAACCCCCCCTATGTGCCCGGGGTGGTTGGCGGGATCTTGCAGGCAGCCACCATCTGTGCCTCC AGCCACCAATTCCTCTCCACACACTACAACCTCCACAACCTGTACGGCCTCACTGAAGCTATCGCCTCCA GCAGGGCCCTGGTCAAGACTCGGGGAACACGACCCTTTGTGATCTCCCGCTCAACCTTCTCGGGCCACGG CCGGTACGCTGGTCACTGGACAGGGGATGTGCGGAGCTCTTGGGAGCATCTTGCATACTCTGTGCCAGAC ATCCTGCAGTTCAACCTGCTGGGCGTGCCCCTGGTCGGGGCGGACATCTGCGGCTTCATAGGAGACACGT CAGAAGAGCTGTGTGTGCGCTGGACCCAGTTGGGGGCCTTCTACCCCTTCATGCGGAACCACAATGACCT GAATAGCGTGCCTCAGGAGCCGTACAGGTTCAGCGAGACGGCGCAGCAGGCCATGAGGAAGGCCTTCGCC TTACGCTATGCCCTTCTGCCCTACCTGTACACTCTCTTCCACCGCGCCCACGTCAGAGGAGACACGGTGG CCCGGCCCCTCTTCCTGGAGTTCCCTGAGGATCCCAGCACCTGGTCTGTGGACCGCCAGCTCTTGTGGGG GCCGGCCCTGCTCATCACACCTGTGCTTGAGCCTGGGAAAACTGAAGTGACGGGCTACTTCCCCAAGGGC ACGTGGTACAACATGCAGGTGGTGTCAGTGGATTCCCTCGGTACTCTCCCTTCTCCATCATCGGCTTCAT CCTTCAGATCTGCTGTCCAGAGCAAGGGGCAGTGGCTGACACTGGAAGCCCCACTGGATACCATCAACGT GCACCTGAGGGAGGGGTACATCATACCGCTGCAGGGTCCCAGCCTCACAACCACGGAGTCCCGAAAGCAG CCCATGGCTCTGGCTGTGGCATTAACAGCAAGCGGCGAGGCCGATGGGGAGCTGTTCTGGGACGACGGGG AGAGCCTTGCAGTTCTGGAGCGTGGGGCCTACACACTGGTCACCTTCTCAGCCAAGAACAATACCATTGT GAACAAGTTAGTGCGTGTGACCAAGGAGGGAGCTGAGCTACAACTGAGGGAGGTGACCGTCTTGGGAGTG GCCACAGCTCCTACCCAGGTCCTTTCCAACGGCATCCCTGTCTCCAATTTCACCTACAGCCCTGACAACA AGAGCCTGGCCATCCCTGTCTCACTGCTGATGGGAGAGCTGTTTCAAATCAGCTGGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226319 representing NM_008064
Red=Cloning site Green=Tags(s) MNIRKPLCSNSVVGACTLISLTTAVILGHLMLRELMLLPQDLHESSSGLWKTYRPHHQEGYKPGPLHIQE QTEQPKEAPTQCDVPPSSRFDCAPDKGISQEQCEARGCCYVPAGQVLKEPQIGQPWCFFPPSYPSYRLEN LSSTESGYTATLTRTSPTFFPKDVLTLQLEVLMETDSRLHFKIKDPASKRYEVPLETPRVLSQAPSPLYS VEFSEEPFGVIVRRKLGGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLGEHLSPLMLSTDWARITLWN RDTPPSQGTNLYGSHPFYLALEDGGLAHGVFLLNSNAMDVILQPSPALTWRSTGGILDVYVFLGPEPKSV VQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAIVRQVVENMTRTHFPLDVQWNDLDYMDARRDFTFNQDS FADFPDMVRELHQGGRRYMMIVDPAISSAGPAGSYRPYDEGLRRGVFITNETGQPLIGKVWPGTTAFPDF TNPETLDWWQDMVSEFHAQVPFDGMWLDMNEPSNFVRGSQQGCPNNELENPPYVPGVVGGILQAATICAS SHQFLSTHYNLHNLYGLTEAIASSRALVKTRGTRPFVISRSTFSGHGRYAGHWTGDVRSSWEHLAYSVPD ILQFNLLGVPLVGADICGFIGDTSEELCVRWTQLGAFYPFMRNHNDLNSVPQEPYRFSETAQQAMRKAFA LRYALLPYLYTLFHRAHVRGDTVARPLFLEFPEDPSTWSVDRQLLWGPALLITPVLEPGKTEVTGYFPKG TWYNMQVVSVDSLGTLPSPSSASSFRSAVQSKGQWLTLEAPLDTINVHLREGYIIPLQGPSLTTTESRKQ PMALAVALTASGEADGELFWDDGESLAVLERGAYTLVTFSAKNNTIVNKLVRVTKEGAELQLREVTVLGV ATAPTQVLSNGIPVSNFTYSPDNKSLAIPVSLLMGELFQISWS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008064 |
| ORF Size | 2859 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008064.2 |
| RefSeq Size | 3756 bp |
| RefSeq ORF | 2862 bp |
| Locus ID | 14387 |
| UniProt ID | P70699 |
| MW | 106.7 kDa |
| Gene Summary | This gene encodes a lysosomal acid glucosidase that is involved in the degradation of glycogen. The encoded preproprotein undergoes proteolytic processing to generate a mature enzyme that cleaves alpha-1-4 and alpha-1-6 glycosidic bonds of glycogen, maltose and intermediate oligosaccharides within the lysosome. Mice lacking the encoded protein exhibit symptoms similar to human Pompe syndrome such as accumulation of glycogen in cardiac and skeletal muscle lysosomes resulting in reduced mobility and strength. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203732 | Gaa (untagged) - Mouse glucosidase, alpha, acid (Gaa), transcript variant 1, (10ug) |
CNY 10,480.00 |
|
| MG211293 | Gaa (tGFP-tagged) - Mouse glucosidase, alpha, acid (Gaa) |
CNY 10,450.00 |
|
| MG226319 | Gaa (tGFP-tagged) - Mouse glucosidase alpha acid (Gaa) transcript variant 1, (10ug) |
CNY 12,064.00 |
|
| MR226319L3 | Lenti ORF clone of Gaa (Myc-DDK-tagged) - Mouse glucosidase, alpha, acid (Gaa), transcript variant 1 |
CNY 11,400.00 |
|
| MR226319L4 | Lenti ORF clone of Gaa (mGFP-tagged) - Mouse glucosidase, alpha, acid (Gaa), transcript variant 1 |
CNY 11,400.00 |
