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Trim72 (NM_001079932) Mouse Tagged ORF Clone
CAT＃: MR226036
- TrueORF®
Trim72 (Myc-DDK-tagged) - Mouse tripartite motif-containing 72 (Trim72)
ORF Plasmid: tGFP
"NM_001079932" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BC067209; MG53 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226036 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCTGCACCCGGCCTTCTGCGTCAGGAACTGTCCTGCCCACTGTGCTTGCAGCTGTTCGATGCGC CAGTGACGGCTGAGTGTGGCCACAGTTTCTGCCGTGCCTGCCTGATCCGGGTGGCAGGGGAGCCTGCTGC GGACGGCACAGTTGCCTGTCCCTGTTGTCAGGCACCTACACGGCCGCAGGCTCTAAGCACTAACCTCCAG TTGTCACGCCTTGTGGAGGGTTTGGCGCAAGTGCCCCAAGGCCACTGCGAGGAACACCTGGATCCACTGA GCATCTACTGCGAGCAGGACCGCACACTTGTGTGTGGTGTGTGTGCCTCGCTCGGTTCTCACCGTGGTCA TCGTCTCCTGCCTGCCGCTGAAGCCCAAGCACGCCTCAAGACACAGCTTCCACAGCAGAAGATGCAGCTG CAGGAGGCATGCATGCGCAAGGAGAAGACTGTAGCGGTGCTGGAGCATCAGCTGGTGGAGGTGGAGGAGA CAGTGCGCCAGTTCCGGGGAGCTGTCGGGGAGCAGCTGGGGAAGATGCGGATGTTCCTGGCTGCCCTAGA AAGTTCTCTGGACCGTGAAGCAGAAAGGGTTCGGGGTGATGCTGGGGTTGCCTTGCGTCGGGAGCTGTCA AGCCTGAACTCTTACCTAGAGCAACTGAGGCAGATGGAGAAGGTGCTGGAGGAGGTGGCTGACAAGCCAC AGACAGAATTCCTCATGAAATTCTGCCTGGTAACCAGCAGGCTGCAGAAGATCCTGTCAGAGTCACCACC ACCGGCAAGGCTAGATATCCAGCTGCCTGTCATCTCAGATGACTTCAAATTCCAGGTGTGGAAGAAGATG TTCCGGGCTCTGATGCCAGCGCTGGAGGAACTGACTTTTGACCCCAGCTCTGCGCACCCGAGCCTGGTGG TGTCCTCCTCTGGTCGCCGAGTGGAGTGCTCAGACCAGAAGGCGCCGCCAGCGGGAGAAGACACGCGTCA GTTCGACAAGGCAGTAGCGGTGGTGGCGCAGCAGCTGCTGTCACAGGGCGAGCACTATTGGGAGGTGGAG GTGGGCGACAAACCACGCTGGGCCCTGGGAGTGATGGCGGCTGACGCTTCCCGCCGTGGCCGGCTGCACG CGGTGCCCTCACAGGGGCTGTGGCTGCTGGGTCTGCGCGATGGCAAGATCCTGGAGGCGCACGTGGAGGC CAAGGAGCCGCGGGCACTGCGCACCCCAGAGAGGCCTCCGGCGCGCATTGGCCTCTACCTAAGCTTCGCA GATGGCGTCCTGGCTTTCTATGATGCGAGCAACCCCGACGTACTTACGCCAATCTTTTCTTTCCACGAGC GTCTGCCCGGGCCGGTGTACCCCATCTTTGACGTGTGCTGGCACGACAAGGGCAAGAATGCCCAGCCCCT GCTGCTTGTGGGGCCGGAGCAGGAACAGGCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR226036 protein sequence
Red=Cloning site Green=Tags(s) MSAAPGLLRQELSCPLCLQLFDAPVTAECGHSFCRACLIRVAGEPAADGTVACPCCQAPTRPQALSTNLQ LSRLVEGLAQVPQGHCEEHLDPLSIYCEQDRTLVCGVCASLGSHRGHRLLPAAEAQARLKTQLPQQKMQL QEACMRKEKTVAVLEHQLVEVEETVRQFRGAVGEQLGKMRMFLAALESSLDREAERVRGDAGVALRRELS SLNSYLEQLRQMEKVLEEVADKPQTEFLMKFCLVTSRLQKILSESPPPARLDIQLPVISDDFKFQVWKKM FRALMPALEELTFDPSSAHPSLVVSSSGRRVECSDQKAPPAGEDTRQFDKAVAVVAQQLLSQGEHYWEVE VGDKPRWALGVMAADASRRGRLHAVPSQGLWLLGLRDGKILEAHVEAKEPRALRTPERPPARIGLYLSFA DGVLAFYDASNPDVLTPIFSFHERLPGPVYPIFDVCWHDKGKNAQPLLLVGPEQEQA SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001079932 |
| ORF Size | 1434 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001079932.3, NP_001073401.1 |
| RefSeq Size | 2472 bp |
| RefSeq ORF | 1434 bp |
| Locus ID | 434246 |
| UniProt ID | Q1XH17 |
| MW | 52.8 kDa |
| Gene Summary | Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216581 | Trim72 (untagged) - Mouse tripartite motif-containing 72 (Trim72), (10ug) |
CNY 6,180.00 |
|
| MG226036 | Trim72 (tGFP-tagged) - Mouse tripartite motif-containing 72 (Trim72), (10ug) |
CNY 7,088.00 |
|
| MR226036L1 | Lenti ORF clone of Trim72 (Myc-DDK-tagged) - Mouse tripartite motif-containing 72 (Trim72) |
CNY 7,888.00 |
|
| MR226036L2 | Lenti ORF clone of Trim72 (mGFP-tagged) - Mouse tripartite motif-containing 72 (Trim72) |
CNY 6,650.00 |
|
| MR226036L3 | Lenti ORF clone of Trim72 (Myc-DDK-tagged) - Mouse tripartite motif-containing 72 (Trim72) |
CNY 6,650.00 |
|
| MR226036L4 | Lenti ORF clone of Trim72 (mGFP-tagged) - Mouse tripartite motif-containing 72 (Trim72) |
CNY 6,650.00 |
