F8 (NM_007977) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Cf-8; Cf8; FVIII
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR225863 representing NM_007977 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAATAGCACTCTTCGCTTGCTTCTTTCTGAGCCTTTTCAATTTCTGCTCTAGTGCCATCAGAAGAT ACTACCTTGGTGCAGTGGAATTGTCCTGGAACTATATTCAGAGTGATCTGCTCAGTGTGCTGCATACAGA CTCAAGATTTCTTCCTAGAATGTCAACATCTTTTCCATTCAACACCTCCATCATGTATAAAAAGACTGTG TTTGTAGAGTACAAGGACCAGCTTTTCAACATTGCCAAGCCCAGGCCACCCTGGATGGGTTTGCTAGGTC CTACCATTTGGACTGAGGTTCATGACACAGTGGTCATTACACTTAAAAACATGGCTTCTCATCCTGTCAG TCTTCATGCTGTTGGTGTGTCCTACTGGAAAGCTTCTGAGGGAGATGAATATGAAGATCAGACAAGCCAA ATGGAGAAGGAAGATGATAAAGTTTTCCCTGGTGAAAGTCATACTTATGTTTGGCAAGTCCTGAAAGAGA ATGGTCCAATGGCCTCTGACCCTCCATGTCTCACTTACTCATATATGTCTCATGTGGATCTGGTGAAAGA TTTGAATTCAGGCCTCATTGGAGCTCTGCTAGTATGTAAAGAAGGCAGTCTCTCCAAAGAAAGAACACAG ATGTTGTACCAATTTGTACTGCTTTTTGCTGTATTTGATGAAGGGAAGAGCTGGCACTCAGAAACAAACG ACTCTTATACACAGTCTATGGATTCTGCATCTGCTAGAGACTGGCCTAAAATGCACACAGTCAATGGCTA TGTAAACAGGTCTCTTCCAGGTCTGATTGGATGCCATAGGAAATCAGTCTACTGGCACGTGATTGGAATG GGCACCACTCCTGAAATACACTCAATATTCCTCGAAGGTCACACATTTTTTGTGAGGAACCACCGTCAAG CTTCATTGGAGATATCACCAATAACTTTCCTTACTGCTCAAACACTCTTGATAGATCTTGGGCAGTTCCT ACTATTTTGTCATATCTCTTCCCATAAACATGATGGCATGGAAGCTTATGTCAAAGTAGATAGCTGCCCT GAGGAATCCCAATGGCAAAAGAAAAATAATAATGAGGAAATGGAAGATTATGATGATGATCTTTATTCAG AAATGGATATGTTCACATTGGATTATGACAGCTCTCCTTTTATCCAAATTCGCTCGGTTGCTAAAAAGTA CCCTAAAACTTGGATACATTATATTTCTGCTGAGGAGGAAGACTGGGACTATGCACCTTCAGTTCCTACC TCGGATAATGGAAGTTATAAAAGCCAGTATCTGAGCAATGGTCCTCATCGGATTGGTAGGAAATATAAAA AAGTCAGATTTATAGCATACACAGATGAAACCTTTAAGACTCGTGAAACTATTCAGCATGAATCAGGACT CTTGGGACCTTTACTTTATGGAGAAGTTGGAGACACACTGTTGATTATTTTTAAGAATCAAGCAAGCCGA CCATATAACATTTACCCTCATGGAATCACTGATGTCAGTCCTCTACATGCAAGGAGATTGCCAAGAGGTA TAAAGCACGTGAAGGATTTGCCAATTCATCCAGGAGAGATATTCAAGTACAAGTGGACAGTTACAGTAGA AGATGGACCAACTAAATCAGATCCACGGTGCCTGACCCGCTATTATTCAAGTTTCATTAACCCTGAGAGA GATCTAGCTTCAGGACTGATTGGCCCTCTTCTCATCTGCTACAAAGAATCTGTAGATCAAAGGGGAAACC AGATGATGTCAGACAAAAGAAATGTCATCCTGTTTTCTATATTTGATGAGAACCAAAGCTGGTACATCAC AGAGAACATGCAACGCTTCCTCCCCAATGCAGCTAAAACACAGCCCCAGGACCCTGGGTTCCAGGCCTCC AACATCATGCACAGCATCAATGGCTATGTTTTTGATAGCTTGGAGTTGACAGTTTGTTTGCATGAGGTGG CATACTGGCACATTCTCAGTGTTGGAGCACAGACAGACTTCTTATCTATCTTCTTCTCTGGATATACTTT CAAACACAAAATGGTCTATGAAGATACACTTACCCTGTTCCCATTCTCAGGAGAAACTGTCTTTATGTCG ATGGAAAACCCAGGTCTATGGGTCTTGGGGTGTCATAATTCAGACTTTCGGAAGAGAGGTATGACAGCAT TGCTGAAAGTTTCTAGTTGTGACAAGAGCACTAGTGATTATTATGAAGAAATATATGAAGATATTCCAAC ACAGTTGGTGAATGAGAACAATGTCATTGATCCCAGAAGCTTCTTCCAGAATACAAATCATCCTAATACT AGGAAAAAGAAATTCAAAGATTCCACAATTCCAAAAAATGATATGGAGAAGATTGAGCCTCAGTTTGAAG AGATAGCAGAGATGCTTAAAGTACAGAGTGTCTCAGTTAGTGACATGTTGATGCTCTTGGGACAGAGTCA TCCTACTCCACATGGCTTATTTTTATCAGATGGCCAAGAAGCCATCTATGAGGCTATTCATGATGATCAT TCACCAAATGCAATAGACAGCAATGAAGGCCCATCTAAAGTGACCCAACTCAGGCCAGAATCCCATCACA GTGAGAAAATAGTATTTACTCCTCAGCCCGGCCTCCAGTTAAGATCCAATAAAAGTTTGGAGACAACTAT AGAAGTAAAGTGGAAGAAACTTGGTTTGCAAGTTTCTAGTTTGCCAAGTAATCTAATGACTACAACAATT CTGTCAGACAATTTGAAAGCAACTTTTGAAAAGACAGATTCTTCAGGATTTCCAGATATGCCAGTTCACT CTAGTAGTAAATTAAGTACTACTGCATTTGGTAAGAAAGCATATTCCCTTGTTGGGTCTCATGTACCTTT AAACGTGAGTGAAGAAAATAGTGATTCCAACATATTGGATTCAACTTTAATGTATAGTCAAGAAAGTTTA CCAAGAGATAATATATTATCAATGGAGAATGATAGATTACTCAGAGAGAAGAGGTTTCATGGAATTGCTT TATTGACCAAAGATAATACTTTATTCAAAGACAATGTCTCCTTAATGAAAACAAACAAAACATATAATCA TTCAACAACTAATGAAAAACTACACACTGAGAGCCCAACATCAATTGAGAATAGTACAACAGACTTGCAA GATGCCATATTAAAGGTCAATAGTGAGATTCAAGAAGTAACAGCTTTGATTCATGATGGAACACTTTTAG GCAAAAATTCTACATATTTGAGACTAAACCATATGCTAAATAGAACTACCTCAACAAAAAATAAAGACAT ATTTCATAGAAAAGATGAAGATCCTATTCCACAAGATGAAGAGAATACAATCATGCCATTTTCCAAGATG TTGTTCTTGTCAGAATCTTCAAATTGGTTTAAAAAGACCAATGGAAATAATTCCTTGAACTCTGAGCAAG AACATAGTCCAAAGCAATTAGTATATTTAATGTTTAAAAAATATGTAAAAAATCAAAGTTTCTTGTCAGA GAAAAATAAAGTCACAGTAGAACAGGATGGATTTACAAAGAACATAGGACTTAAAGACATGGCTTTTCCA CATAATATGAGCATATTTCTTACCACTTTGTCTAACGTACATGAAAATGGTAGGCACAATCAAGAAAAAA ATATTCAGGAAGAGATAGAGAAGGAAGCACTAATTGAAGAGAAAGTAGTTTTGCCCCAGGTGCACGAAGC AACTGGCTCTAAGAATTTCTTGAAAGACATATTGATACTAGGCACTAGGCAAAATATAAGTTTATATGAA GTACATGTACCAGTACTTCAAAACATCACATCAATAAACAATTCAACAAATACAGTACAGATTCACATGG AGCATTTCTTTAAAAGAAGGAAGGACAAGGAAACAAATTCAGAAGGCTTGGTAAATAAAACCAGAGAAAT GGTAAAAAACTATCCAAGCCAGAAGAATATTACTACTCAACGTAGTAAACGGGCTTTGGGACAATTCAGA CTGTCAACTCAATGGCTTAAAACCATAAACTGTTCAACACAGTGTATCATTAAACAGATAGACCACAGCA AGGAAATGAAAAAGTTCATTACTAAATCTTCCTTATCAGATTCTTCTGTGATTAAAAGCACCACTCAGAC AAATAGTTCTGACTCACACATTGTAAAAACATCAGCATTTCCACCAATAGATCTCAAAAGGAGTCCATTC CAAAACAAATTTTCTCATGTTCAAGCATCATCCTACATTTATGACTTTAAGACAAAAAGTTCAAGAATTC AAGAAAGCAATAATTTCTTAAAAGAAACCAAAATAAATAACCCTTCTTTAGCCATTCTACCATGGAATAT GTTCATAGATCAAGGAAAATTTACCTCCCCAGGGAAAAGTAACACAAACTCAGTCACATATAAGAAACGT GAGAACATTATTTTCTTGAAACCAACTTTGCCTGAAGAATCTGGCAAAATTGAATTGCTTCCTCAAGTTT CCATTCAAGAGGAAGAAATTTTACCTACAGAAACTAGCCATGGATCTCCTGGACACTTGAATCTCATGAA AGAGGTCTTTCTTCAGAAAATACAGGGGCCTACTAAATGGAATAAAGCAAAGAGGCATGGAGAAAGTATA AAAGGTAAAACAGAGAGCTCTAAAAATACTCGCTCAAAACTGCTAAATCATCATGCTTGGGATTATCATT ATGCTGCACAGATACCAAAAGATATGTGGAAATCCAAAGAGAAGTCACCAGAAATTATATCCATTAAGCA AGAGGACACCATTTTGTCTCTGAGGCCTCATGGAAACAGTCATTCAATAGGGGCAAATGAGAAACAAAAT TGGCCTCAAAGAGAAACCACTTGGGTAAAGCAAGGCCAAACTCAAAGGACATGCTCTCAAATCCCACCAG TGTTGAAACGACATCAAAGGGAACTTAGTGCTTTTCAATCAGAACAAGAAGCAACTGACTATGATGATGC CATCACCATTGAAACAATCGAGGATTTTGACATTTACAGTGAGGACATAAAGCAAGGTCCCCGCAGCTTT CAACAGAAAACAAGGCACTATTTTATTGCAGCTGTGGAACGACTCTGGGACTATGGGATGAGTACATCTC ATGTTCTACGAAATAGGTATCAAAGTGACAATGTACCTCAGTTCAAGAAAGTAGTTTTCCAGGAATTTAC TGATGGCTCCTTTAGTCAGCCCTTATATCGTGGAGAATTAAATGAACACCTGGGGTTGTTGGGCCCATAT ATAAGAGCAGAAGTTGAAGACAACATTATGGTAACTTTCAAAAACCAGGCCTCCCGTCCCTACTCCTTCT ATTCTAGCCTCATTTCTTATAAAGAAGATCAGAGAGGAGAAGAACCTAGAAGAAACTTTGTCAAGCCTAA TGAAACCAAAATTTATTTTTGGAAAGTACAACATCATATGGCACCCACAGAAGATGAGTTTGACTGCAAG GCCTGGGCTTATTTCTCTGATGTTGATCTTGAAAGAGATATGCACTCGGGATTAATTGGACCCCTTCTGA TTTGCCACGCGAACACACTGAATCCTGCTCATGGGAGACAAGTGTCAGTACAGGAATTTGCTCTGCTTTT CACTATCTTTGATGAGACCAAGAGCTGGTACTTCACTGAAAACGTGAAAAGGAACTGCAAGACACCCTGC AATTTCCAGATGGAAGACCCCACTTTGAAAGAGAATTATCGCTTCCATGCAATCAATGGTTATGTAATGG ATACCCTACCAGGCTTAGTAATGGCTCAAGATCAAAGGATTCGATGGTATCTTCTCAGCATGGGCAACAA TGAGAACATCCAATCTATTCATTTCAGTGGACATGTTTTCACTGTACGGAAAAAAGAGGAGTATAAAATG GCAGTGTACAACCTCTACCCAGGTGTTTTTGAGACTCTGGAAATGATACCATCCAGAGCTGGAATATGGC GAGTAGAATGCCTTATTGGCGAGCACTTACAGGCTGGGATGAGCACTCTTTTTCTGGTGTACAGCAAGCA GTGTCAGATTCCTCTTGGAATGGCTTCTGGAAGCATCCGTGATTTCCAGATTACAGCTTCAGGACATTAT GGACAGTGGGCCCCAAACCTGGCAAGACTTCATTATTCCGGATCAATCAATGCCTGGAGTACCAAGGAGC CCTTTTCTTGGATCAAGGTAGATCTGTTGGCACCAATGATTGTTCATGGCATCAAGACTCAGGGTGCTCG TCAGAAATTTTCCAGCCTTTATATCTCTCAATTTATCATCATGTATAGCCTGGATGGGAAGAAGTGGCTG AGTTATCAAGGAAATTCCACTGGAACCTTAATGGTTTTCTTTGGCAATGTGGACTCATCTGGGATTAAGC ATAATAGTTTTAATCCTCCAATTATTGCTCGATATATCCGTTTGCACCCCACTCATTCTAGCATCCGTAG TACTCTTCGCATGGAGTTGATGGGCTGTGATTTAAACAGTTGCAGCATACCATTGGGAATGGAAAGTAAA GTAATATCAGATACACAAATCACTGCCTCATCCTACTTCACCAACATGTTTGCTACTTGGTCTCCTTCAC AAGCTCGACTTCACCTCCAGGGAAGGACTAATGCCTGGCGACCTCAGGTGAATGATCCAAAACAATGGTT GCAAGTGGACTTACAAAAGACAATGAAAGTCACTGGAATAATAACCCAGGGAGTGAAATCTCTCTTTACC AGCATGTTTGTGAAAGAGTTCCTTATTTCCAGCAGTCAAGATGGCCATCACTGGACTCAAATTTTATACA ATGGCAAGGTAAAGGTTTTTCAGGGGAATCAGGACTCATCCACACCTATGATGAATTCTCTAGACCCACC ATTACTCACTCGCTATCTTCGAATTCACCCCCAGATCTGGGAGCACCAAATTGCTCTGAGGCTTGAGATT CTAGGATGTGAGGCCCAGCAGCAATAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR225863 representing NM_007977 Red=Cloning site Green=Tags(s) MQIALFACFFLSLFNFCSSAIRRYYLGAVELSWNYIQSDLLSVLHTDSRFLPRMSTSFPFNTSIMYKKTV FVEYKDQLFNIAKPRPPWMGLLGPTIWTEVHDTVVITLKNMASHPVSLHAVGVSYWKASEGDEYEDQTSQ MEKEDDKVFPGESHTYVWQVLKENGPMASDPPCLTYSYMSHVDLVKDLNSGLIGALLVCKEGSLSKERTQ MLYQFVLLFAVFDEGKSWHSETNDSYTQSMDSASARDWPKMHTVNGYVNRSLPGLIGCHRKSVYWHVIGM GTTPEIHSIFLEGHTFFVRNHRQASLEISPITFLTAQTLLIDLGQFLLFCHISSHKHDGMEAYVKVDSCP EESQWQKKNNNEEMEDYDDDLYSEMDMFTLDYDSSPFIQIRSVAKKYPKTWIHYISAEEEDWDYAPSVPT SDNGSYKSQYLSNGPHRIGRKYKKVRFIAYTDETFKTRETIQHESGLLGPLLYGEVGDTLLIIFKNQASR PYNIYPHGITDVSPLHARRLPRGIKHVKDLPIHPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINPER DLASGLIGPLLICYKESVDQRGNQMMSDKRNVILFSIFDENQSWYITENMQRFLPNAAKTQPQDPGFQAS NIMHSINGYVFDSLELTVCLHEVAYWHILSVGAQTDFLSIFFSGYTFKHKMVYEDTLTLFPFSGETVFMS MENPGLWVLGCHNSDFRKRGMTALLKVSSCDKSTSDYYEEIYEDIPTQLVNENNVIDPRSFFQNTNHPNT RKKKFKDSTIPKNDMEKIEPQFEEIAEMLKVQSVSVSDMLMLLGQSHPTPHGLFLSDGQEAIYEAIHDDH SPNAIDSNEGPSKVTQLRPESHHSEKIVFTPQPGLQLRSNKSLETTIEVKWKKLGLQVSSLPSNLMTTTI LSDNLKATFEKTDSSGFPDMPVHSSSKLSTTAFGKKAYSLVGSHVPLNVSEENSDSNILDSTLMYSQESL PRDNILSMENDRLLREKRFHGIALLTKDNTLFKDNVSLMKTNKTYNHSTTNEKLHTESPTSIENSTTDLQ DAILKVNSEIQEVTALIHDGTLLGKNSTYLRLNHMLNRTTSTKNKDIFHRKDEDPIPQDEENTIMPFSKM LFLSESSNWFKKTNGNNSLNSEQEHSPKQLVYLMFKKYVKNQSFLSEKNKVTVEQDGFTKNIGLKDMAFP HNMSIFLTTLSNVHENGRHNQEKNIQEEIEKEALIEEKVVLPQVHEATGSKNFLKDILILGTRQNISLYE VHVPVLQNITSINNSTNTVQIHMEHFFKRRKDKETNSEGLVNKTREMVKNYPSQKNITTQRSKRALGQFR LSTQWLKTINCSTQCIIKQIDHSKEMKKFITKSSLSDSSVIKSTTQTNSSDSHIVKTSAFPPIDLKRSPF QNKFSHVQASSYIYDFKTKSSRIQESNNFLKETKINNPSLAILPWNMFIDQGKFTSPGKSNTNSVTYKKR ENIIFLKPTLPEESGKIELLPQVSIQEEEILPTETSHGSPGHLNLMKEVFLQKIQGPTKWNKAKRHGESI KGKTESSKNTRSKLLNHHAWDYHYAAQIPKDMWKSKEKSPEIISIKQEDTILSLRPHGNSHSIGANEKQN WPQRETTWVKQGQTQRTCSQIPPVLKRHQRELSAFQSEQEATDYDDAITIETIEDFDIYSEDIKQGPRSF QQKTRHYFIAAVERLWDYGMSTSHVLRNRYQSDNVPQFKKVVFQEFTDGSFSQPLYRGELNEHLGLLGPY IRAEVEDNIMVTFKNQASRPYSFYSSLISYKEDQRGEEPRRNFVKPNETKIYFWKVQHHMAPTEDEFDCK AWAYFSDVDLERDMHSGLIGPLLICHANTLNPAHGRQVSVQEFALLFTIFDETKSWYFTENVKRNCKTPC NFQMEDPTLKENYRFHAINGYVMDTLPGLVMAQDQRIRWYLLSMGNNENIQSIHFSGHVFTVRKKEEYKM AVYNLYPGVFETLEMIPSRAGIWRVECLIGEHLQAGMSTLFLVYSKQCQIPLGMASGSIRDFQITASGHY GQWAPNLARLHYSGSINAWSTKEPFSWIKVDLLAPMIVHGIKTQGARQKFSSLYISQFIIMYSLDGKKWL SYQGNSTGTLMVFFGNVDSSGIKHNSFNPPIIARYIRLHPTHSSIRSTLRMELMGCDLNSCSIPLGMESK VISDTQITASSYFTNMFATWSPSQARLHLQGRTNAWRPQVNDPKQWLQVDLQKTMKVTGIITQGVKSLFT SMFVKEFLISSSQDGHHWTQILYNGKVKVFQGNQDSSTPMMNSLDPPLLTRYLRIHPQIWEHQIALRLEI LGCEAQQQY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_007977
ORF Size	6957 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_007977.2, NP_032003.2
RefSeq Size	7522 bp
RefSeq ORF	6960 bp
Locus ID	14069
UniProt ID	Q06194
MW	266.6 kDa
Gene Summary	Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.[UniProtKB/Swiss-Prot Function]

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