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Dnmt3b (NM_001003960) Mouse Tagged Lenti ORF Clone
CAT＃: MR225600L4
- LentiORF®
Lenti ORF clone of Dnmt3b (mGFP-tagged) - Mouse DNA methyltransferase 3B (Dnmt3b), transcript variant 2
"NM_001003960" in other vectors (4)
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CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | MmuIIIB |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR225600).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001003960 |
| ORF Size | 2517 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001003960.3, NP_001003960.2 |
| RefSeq Size | 4281 bp |
| RefSeq ORF | 2520 bp |
| Locus ID | 13436 |
| UniProt ID | O88509 |
| Gene Summary | This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene results in severe developmental defects and loss of viability. Mutation of the related gene in humans causes immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. There is a pseudogene for this gene located adjacent to this gene in the same region of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221993 | Dnmt3b (untagged) - Mouse DNA methyltransferase 3B (Dnmt3b), transcript variant 2, (10ug) |
CNY 10,740.00 |
|
| MG225600 | Dnmt3b (tGFP-tagged) - Mouse DNA methyltransferase 3B (Dnmt3b) transcript variant 2, (10ug) |
CNY 7,890.00 |
|
| MR225600 | Dnmt3b (Myc-DDK-tagged) - Mouse DNA methyltransferase 3B (Dnmt3b), transcript variant 2 |
CNY 6,144.00 |
|
| MR225600L3 | Lenti ORF clone of Dnmt3b (Myc-DDK-tagged) - Mouse DNA methyltransferase 3B (Dnmt3b), transcript variant 2 |
CNY 9,030.00 |
