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Mcoln1 (NM_053177) Mouse Tagged ORF Clone
CAT＃: MR225390
- TrueORF®
Mcoln1 (Myc-DDK-tagged) - Mouse mucolipin 1 (Mcoln1)
ORF Plasmid: tGFP
"NM_053177" in other vectors (4)
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CNY 6,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2210015I05Rik; mucolipidin; TRPML1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225390 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCCCGGCGGGCCGGCGCGCCTCAGAGACTGAGCGGCTGCTGACCCCCAATCCTGGGTATGGGA CCCAGGTGGGGACCTCACCAGCCCCAACAACCCCCACAGAAGAGGAAGACCTCCGCCGCCGCCTCAAGTA CTTCTTTATGAGCCCATGTGACAAGTTCCGGGCCAAAGGCCGCAAGCCCTGCAAGCTGATGCTGCAGGTG GTCAAGATCTTGGTGGTCACTGTGCAGCTCATTCTCTTTGGGCTCAGCAACCAGCTGGTGGTGACATTCC GGGAAGAGAACACCATTGCCTTCCGACATCTCTTCCTGCTGGGTTACTCTGATGGGTCTGATGACACCTT TGCAGCCTACACACAGGAGCAGCTCTACCAAGCCATCTTCTATGCTGTGGACCAGTACCTGATACTACCT GAGATATCCCTGGGCCGGTATGCCTATGTCCGTGGTGGGGGTGGGCCTTGGGCCAATGGATCAGCTTTGG CTCTCTGCCAGCGGTACTACCACCGTGGCCATGTGGACCCAGCCAATGATACCTTTGACATTGATCCAAG GGTAGTCACTGACTGTATCCAGGTGGATCCTCCTGACAGACCCCCTGACATCCCCAGTGAGGACTTGGAC TTCTTGGATGGCAGCGCCAGTTACAAGAACCTCACACTGAAATTCCACAAGCTGATCAACGTCACCATCC ACTTCCAGCTGAAGACAATTAACCTGCAGAGCCTCATCAACAATGAGATCCCTGATTGTTACACCTTCAG TATCCTGATCACATTTGACAATAAAGCGCACAGTGGGCGAATCCCCATCCGCCTGGAGACCAAGACCCAC ATCCAGGAGTGCAAACACCCCAGTGTCTCCAGACATGGAGACAACAGCTTCCGGCTTCTGTTTGATGTGG TGGTTATCCTCACCTGCTCCCTGTCCTTCCTGCTGTGCGCCCGCTCACTGCTCCGTGGCTTCCTGCTGCA GAACGAGTTTGTTGTATTCATGTGGCGGCGGCGGGGTCGGGAAATCAGCCTCTGGGAACGGCTGGAGTTT GTCAATGGCTGGTACATCCTGCTGGTCACCAGTGACGTGCTCACCATCTCGGGGACTGTCATGAAGATTG GCATTGAGGCAAAGAACCTAGCCAGCTATGATGTCTGCAGTATTCTCTTGGGTACCTCCACTCTGCTAGT CTGGGTCGGTGTCATTCGCTACCTGACATTTTTCCACAAGTACAACATCTTGATTGCCACGTTGCGAGTG GCACTGCCCAGTGTCATGCGTTTCTGCTGCTGTGTGGCTGTCATCTACCTGGGCTATTGCTTCTGTGGCT GGATCGTTCTAGGGCCCTACCATGTGAAGTTCCGCTCGCTGTCCATGGTTTCTGAGTGTCTGTTCTCACT CATCAACGGAGACGACATGTTTGTGACGTTCGCGGCCATGCAGGCCCAGCAGGGTCACAGCAGCCTGGTG TGGCTCTTCTCCCAGCTGTACCTCTACTCCTTCATCAGCCTCTTCATCTACATGGTGCTGAGCCTCTTCA TTGCACTCATCACCGGCGCCTATGACACCATCAAGCACCCAGGAGGTACTGGCACAGAGAAGAGTGAGCT CCAGGCCTACATCGAGCAGTGCCAGGATAGCCCCACATCTGGCAAGTTCCGTCGTGGCAGTGGCTCAGCT TGTAGCCTTTTCTGCTGCTGTGGAAGGGACTCCCCGGAGGACCATTCGCTGCTGGTGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225390 protein sequence
Red=Cloning site Green=Tags(s) MATPAGRRASETERLLTPNPGYGTQVGTSPAPTTPTEEEDLRRRLKYFFMSPCDKFRAKGRKPCKLMLQV VKILVVTVQLILFGLSNQLVVTFREENTIAFRHLFLLGYSDGSDDTFAAYTQEQLYQAIFYAVDQYLILP EISLGRYAYVRGGGGPWANGSALALCQRYYHRGHVDPANDTFDIDPRVVTDCIQVDPPDRPPDIPSEDLD FLDGSASYKNLTLKFHKLINVTIHFQLKTINLQSLINNEIPDCYTFSILITFDNKAHSGRIPIRLETKTH IQECKHPSVSRHGDNSFRLLFDVVVILTCSLSFLLCARSLLRGFLLQNEFVVFMWRRRGREISLWERLEF VNGWYILLVTSDVLTISGTVMKIGIEAKNLASYDVCSILLGTSTLLVWVGVIRYLTFFHKYNILIATLRV ALPSVMRFCCCVAVIYLGYCFCGWIVLGPYHVKFRSLSMVSECLFSLINGDDMFVTFAAMQAQQGHSSLV WLFSQLYLYSFISLFIYMVLSLFIALITGAYDTIKHPGGTGTEKSELQAYIEQCQDSPTSGKFRRGSGSA CSLFCCCGRDSPEDHSLLVN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053177 |
| ORF Size | 1743 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053177.1, NP_444407.1 |
| RefSeq Size | 2039 bp |
| RefSeq ORF | 1743 bp |
| Locus ID | 94178 |
| UniProt ID | Q99J21 |
| MW | 65.5 kDa |
| Gene Summary | Nonselective cation channel probably playing a role in the regulation of membrane trafficking events and of metal homeostasis (PubMed:29019981). Proposed to play a major role in Ca(2+) release from late endosome and lysosome vesicles to the cytoplasm, which is important for many lysosome-dependent cellular events, including the fusion and trafficking of these organelles, exocytosis and autophagy. Required for efficient uptake of large particles in macrophages in which Ca(2+) release from the lysosomes triggers lysosomal exocytosis. May also play a role in phagosome-lysosome fusion (PubMed:23993788). Involved in lactosylceramide trafficking indicative for a role in the regulation of late endocytic membrane fusion/fission events. By mediating lysosomal Ca(2+) release is involved in regulation of mTORC1 signaling and in mTOR/TFEB-dependent lysosomal adaptation to environmental cues such as nutrient levels (PubMed:25733853). Seems to act as lysosomal active oxygen species (ROS) sensor involved in ROS-induced TFEB activation and autophagy (By similarity). Functions as a Fe(2+) permeable channel in late endosomes and lysosomes. Proposed to play a role in zinc homeostasis probably implicating its association with TMEM163 (By similarity). In adaptive immunity, TRPML2 and TRPML1 may play redundant roles in the function of the specialized lysosomes of B cells (PubMed:17050035).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219324 | Mcoln1 (untagged) - Mouse mucolipin 1 (Mcoln1), (10ug) |
CNY 6,488.00 |
|
| MG225390 | Mcoln1 (tGFP-tagged) - Mouse mucolipin 1 (Mcoln1), (10ug) |
CNY 8,088.00 |
|
| MR225390L3 | Lenti ORF clone of Mcoln1 (Myc-DDK-tagged) - Mouse mucolipin 1 (Mcoln1) |
CNY 7,700.00 |
|
| MR225390L4 | Lenti ORF clone of Mcoln1 (mGFP-tagged) - Mouse mucolipin 1 (Mcoln1) |
CNY 7,700.00 |
