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Cyp7a1 (NM_007824) Mouse Tagged ORF Clone
CAT＃: MR224092
- TrueORF®
Cyp7a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 7, subfamily a, polypeptide 1 (Cyp7a1)
ORF Plasmid: tGFP
"NM_007824" in other vectors (4)
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CNY 3,744.00
CNY 4,370.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224092 representing NM_007824
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAGCATTTCTTTGATCTGGGGGATTGCTGTGGTAGTGAGCTGTTGCATATGGTTTATCATTGGAA TAAGGAGAAGGAAAGTAGGTGAACCTCCTTTGGACAACGGGTTGATTCCATACCTGGGCTGTGCTCTGAA GTTCGGATCCAATCCTCTTGAATTCCTAAGAGCAAAGCAAAGGAAACATGGCCATGTTTTTACCTGCAAA CTGATGGGGAAATATGTCCACTTCATCACAAACTCCCTGTCATACCACAAAGTCTTATGTCACGGAAAAT ATTTTGACTGGAAAAAATTTCATTACACTACTTCTGCGAAGGCATTTGGACACAGAAGCATAGACCCAAG TGATGGAAATACCACGGAAAACATAAACAAGACTTTTAACAAAACCCTCCAGGGAGATGCTCTGTGTTCA CTCTCTGAAGCCATGATGCAAAACCTCCAATCTGTCATGAGACCTCCGGGCCTTCCTAAATCAAAGAGCG CTGTCTGGGTCACGGAAGGGATGTATGCCTTCTGCTACCGAGTGATGTTTGAAGCCGGATATCTAACGCT GTTTGGCAAAGATATTTCAAAGACAGACTCACAAAGAGCATTTATTCAAAACAACCTTGACAGCTTCAAA CAATTTGACCAAGTATTTCCGGCACTAGTGGCGGGCGTCCCTATTCACTTGTTCAAGACCGCACATAAAG CCCGGGAAAGGCTGGCTGAGAGCTTGAAGCACAAGAACCTGTACATGAGGGACCAGGTCTCTGAACTGAT CCGTCTACGCATGTTTCTCAACGATACACTCTCCACCTTTGATGACATGGAGAAGGCTAAGACGCACCTC GTGATCCTCTGGGCATCTCAAGCAAACACCATTCCTGCAACCTTCTGGAGCTTATTTCAAATGATCAGGA GCCCTGAAGCAATGAAAGCAGCCTCTGAAGAAGTGAATGGAGCATTACAGAGTGCTGGCCAAGAGCTCAG CTCTGGAGGGAATGCCATTTACTTGGATCAAGAGCAACTAAACAACCTGCCAGTACTAGATAGCATCATC AAGGAGGCTCTGCGGCTCTCCAGTGCATCCTTGAATATCCGGACAGCTAAGGAGGACTTCACTCTACACC TTGAGGATGGTTCCTATAACATTCGAAAAGATGACATCATAGCTCTTTACCCACAGTTAATGCACTTGGA TCCTGAAATCTACCCAGACCCTTTGACTTTTAAATACGACCGGTACCTTGATGAAAGTGGGAAAGCAAAG ACCACCTTCTATAGAAATGGAAACAAGCTGAAGTATTTCTACATGCCCTTTGGATCAGGAGCTACAATAT GTCCTGGAAGACTATTTGCTGTCCAAGAAATCAAGCAATTTTTGATTCTGATGCTGTCTTACTTTGAACT GGAGCTTGTAGAGAGCCACACCAAGTGTCCCCCTCTAGATCAGTCCCGGGCAGGCTTGGGAATTTTGCCA CCACTAAATGATATTGAGTTTAAATATAAACTGAAACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224092 representing NM_007824
Red=Cloning site Green=Tags(s) MMSISLIWGIAVVVSCCIWFIIGIRRRKVGEPPLDNGLIPYLGCALKFGSNPLEFLRAKQRKHGHVFTCK LMGKYVHFITNSLSYHKVLCHGKYFDWKKFHYTTSAKAFGHRSIDPSDGNTTENINKTFNKTLQGDALCS LSEAMMQNLQSVMRPPGLPKSKSAVWVTEGMYAFCYRVMFEAGYLTLFGKDISKTDSQRAFIQNNLDSFK QFDQVFPALVAGVPIHLFKTAHKARERLAESLKHKNLYMRDQVSELIRLRMFLNDTLSTFDDMEKAKTHL VILWASQANTIPATFWSLFQMIRSPEAMKAASEEVNGALQSAGQELSSGGNAIYLDQEQLNNLPVLDSII KEALRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDIIALYPQLMHLDPEIYPDPLTFKYDRYLDESGKAK TTFYRNGNKLKYFYMPFGSGATICPGRLFAVQEIKQFLILMLSYFELELVESHTKCPPLDQSRAGLGILP PLNDIEFKYKLKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007824 |
| ORF Size | 1509 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007824.3 |
| RefSeq Size | 4172 bp |
| RefSeq ORF | 1512 bp |
| Locus ID | 13122 |
| UniProt ID | Q64505 |
| MW | 57.7 kDa |
| Gene Summary | Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216953 | Cyp7a1 (untagged) - Mouse cytochrome P450, family 7, subfamily a, polypeptide 1 (Cyp7a1), (10ug) |
CNY 6,460.00 |
|
| MG224092 | Cyp7a1 (tGFP-tagged) - Mouse cytochrome P450 family 7 subfamily a polypeptide 1 (Cyp7a1), (10ug) |
CNY 4,850.00 |
|
| MR224092L3 | Lenti ORF clone of Cyp7a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 7, subfamily a, polypeptide 1 (Cyp7a1) |
CNY 6,144.00 |
|
| MR224092L4 | Lenti ORF clone of Cyp7a1 (mGFP-tagged) - Mouse cytochrome P450, family 7, subfamily a, polypeptide 1 (Cyp7a1) |
CNY 6,270.00 |
