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Hey2 (NM_013904) Mouse Tagged ORF Clone
CAT＃: MR223872
- TrueORF®
Hey2 (Myc-DDK-tagged) - Mouse hairy/enhancer-of-split related with YRPW motif 2 (Hey2)
ORF Plasmid: tGFP
"NM_013904" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | bHLHb32; CHF1; Herp1; hesr2; Hrt2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223872 representing NM_013904
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGCCCTTGTGAGGAAACGACCTCCGAAAGCGACCTGGACGAGACCATCGACGTGGGGAGCGAGA ACAATTACCCTGGGCACGCTACAAGCTCAGTGATGAGGTCCAATTCACCGACAACTACCTCTCAGATTAT GGCAAGAAAGAAAAGGAGAGGGATCATAGAAAAAAGGCGTCGGGATCGAATAAATAACAGTTTATCTGAA TTGAGAAGACTAGTGCCAACAGCTTTTGAAAAACAAGGATCTGCCAAGTTAGAAAAGGCTGAAATATTGC AAATGACAGTGGATCATTTGAAGATGCTCCAGGCTACAGGGGGTAAAGGCTACTTTGATGCCCATGCTCT TGCCACAGACTTCATGAGCATTGGATTCCGAGAGTGCTTGACAGAAGTGGCTAGGTACCTAAGCTCAGTG GAAGGCCTTGACCCGTCGGACCCACTACGCGTGCGCCTTGTCTCTCATCTCAGCACCTGTGCCTCCCAGC GGGAGGCAGCAGTGATGACATCCTCCATGGCCCACCACCATCACCCCTTGCACCCTCACCACTGGGCAGC TGCTTTCCACCATCTCCCCACAGCCCTGCTCCAGCCCAATGGACTCCACACATCAGAGTCAACCCCATGT CGCCTATCCACATCTTCAGAAGTGCCTTCTGCTCATGGCTCTGCTCTCCTCACAGCAACGTTTGCCCATG CAGATTCTGCTCTTCGGATGCCATCAGGGGGCACCGTTGCACCCTGCGTGCCACCTCTCTCCACCTCTCT TCTGTCTCTTTCGGCCACTGTGCATGCCGCAGCTGCAGCAGCCACTGCAGCTGCACACAGCTTCCCTCTG TCCTTCGCAGGGGCTTTTCCCATGCTCCCGTCCAATGCAGCGGCAGCAGCCGCTGTTGCTGCTGCAACAG CAATCAGCCCACCCTTGTCGGTATCCGCAGCCTCCAGTCCTCAGCAGACAAGCACTGGGACAAACAATAA ACCTTACCAACCCTGGGGGACAGAAGTTGGAGCCTTT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013904 |
| ORF Size | 1017 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013904.1, NP_038932.1 |
| RefSeq Size | 2549 bp |
| RefSeq ORF | 1020 bp |
| Locus ID | 15214 |
| UniProt ID | Q9QUS4 |
| MW | 36.3 kDa |
| Gene Summary | Transcriptional repressor which functions as a downstream effector of Notch signaling in cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. May be required in conjunction with HEY1 to specify arterial cell fate or identity. Promotes maintenance of neuronal precursor cells and glial versus neuronal fate specification. Binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6 and by the neuronal bHLH factors ASCL1/MASH1 and NEUROD4/MATH3.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208661 | Hey2 (untagged) - Mouse hairy/enhancer-of-split related with YRPW motif 2 (Hey2), (10ug) |
CNY 3,990.00 |
|
| MG223872 | Hey2 (tGFP-tagged) - Mouse hairy/enhancer-of-split related with YRPW motif 2 (Hey2), (10ug) |
CNY 3,710.00 |
|
| MR223872L3 | Lenti ORF clone of Hey2 (Myc-DDK-tagged) - Mouse hairy/enhancer-of-split related with YRPW motif 2 (Hey2) |
CNY 5,230.00 |
|
| MR223872L4 | Lenti ORF clone of Hey2 (mGFP-tagged) - Mouse hairy/enhancer-of-split related with YRPW motif 2 (Hey2) |
CNY 5,230.00 |
