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Lyar (NM_025281) Mouse Tagged ORF Clone
CAT＃: MR222894
- TrueORF®
Lyar (Myc-DDK-tagged) - Mouse Ly1 antibody reactive clone (Lyar)
ORF Plasmid: tGFP
"NM_025281" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MLZ-264 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222894 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTATTTTTTACATGCAATGCATGTGGTGAATCCGTGAAGAAAATACAAGTGGAAAAGCATGTGTCTA ACTGCAGAAACTGTGAATGTCTTTCCTGCATTGACTGTGGGAAAGATTTCTGGGGTGATGACTACAAAAG CCATGTGAAGTGCATCAGTGAAGGTCAGAAGTACGGAGGCAAAGGCTATGAAGCCAAGACACACAAAGGT GATGCAAAACAGCAGGCATGGATTCAGAAAATTAATGAGTTAATAAAGAAACCCAACGTCAGCCCCAAGG TGCGAGAACTTTTGCAGCAAATCAGTGCTTTTGATAATGTTCCCAGAAAAAAGGCGAAGTTTCAGAACTG GATGAAAAACAGCCTGAAAGTGCACAGCGACTCCGTTCTAGAGCAGGTGTGGGATATCTTCTCCGAAGCA TCCAGCAGTGAGCAAGATCAGCAGCAGCCACCCAGCCACACGGCCAAGCCACATGCAGAGATGCCGATCA CTAAAGTTCCATCTGCAAAAACAAATGGCACCACAGAAGAGCAAACCGAGGCGAAGAAGAATAAAAGAGA AAGGAAGGAGGAACGGCAGAAAAACAGGAAGAAAGAGAAGAAAGAGCTGAAGCTAGAAAACCACCAGGAA AACCTGAGGGGCCAGAAGCCAAAGAAGCGCAAGAAGAACCAGGAGGCCGGACATGAGGCTGCTGGGGAGG AGGCCGCCGAGGCCAGCGGCCCACCGGAGAAGAAGAAAGCCCAGGGAGGACAGGCTTCTGAAGAGGGAGC AGACAGAAATGGGGGCCCTGGGGAGGATGCTGCCGAGGGGCAGACCAAGACAGCAGCAGGGAAACGGAAG CGGCCAAAGCACTCAGGAGCTGAGTCTGGTTACAAGAAAAAAAAGATGAAGTTACCAGAGCAGCCTGAAG AGGGAGAGGCCAAGGACCACGAGGCTCCATCGAAAGGTAAATTCAACTGGAAGGGGACCATTAAGGCTGT TTTGAAACAGGCTCCAGACAACGAGATATCAGTCAAGAAGTTAAAGAAAAAGGTTATAGCTCAGTACCAT GCGGTGATGAACGACCATCACACATCGGAGGAGGAGCTCCTGGCCATCTTCAACAGGAAGATCAGCAGGA ACCCCACCTTTAAGGTGCTGAAGGACAGAGTAAAGCTTCTGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222894 protein sequence
Red=Cloning site Green=Tags(s) MVFFTCNACGESVKKIQVEKHVSNCRNCECLSCIDCGKDFWGDDYKSHVKCISEGQKYGGKGYEAKTHKG DAKQQAWIQKINELIKKPNVSPKVRELLQQISAFDNVPRKKAKFQNWMKNSLKVHSDSVLEQVWDIFSEA SSSEQDQQQPPSHTAKPHAEMPITKVPSAKTNGTTEEQTEAKKNKRERKEERQKNRKKEKKELKLENHQE NLRGQKPKKRKKNQEAGHEAAGEEAAEASGPPEKKKAQGGQASEEGADRNGGPGEDAAEGQTKTAAGKRK RPKHSGAESGYKKKKMKLPEQPEEGEAKDHEAPSKGKFNWKGTIKAVLKQAPDNEISVKKLKKKVIAQYH AVMNDHHTSEEELLAIFNRKISRNPTFKVLKDRVKLLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025281 |
| ORF Size | 1167 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025281.3, NP_079557.2 |
| RefSeq Size | 1568 bp |
| RefSeq ORF | 1167 bp |
| Locus ID | 17089 |
| UniProt ID | Q08288 |
| MW | 43.7 kDa |
| Gene Summary | Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs (By similarity). Also acts at the level of transcription regulation. Along with PRMT5, binds embryonic globin promoter (By similarity). Represses the expression of embryonic globin Hbb-y gene (PubMed:25092918). In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and that encoding the small nucleolar RNA SNORD41 (By similarity). Preferentially binds to a DNA motif containing 5'-GGTTAT-3' (By similarity). Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells (PubMed:25735755). Prevents NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal (PubMed:19489080).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208919 | Lyar (untagged) - Mouse Ly1 antibody reactive clone (Lyar), (10ug) |
CNY 3,990.00 |
|
| MG222894 | Lyar (tGFP-tagged) - Mouse Ly1 antibody reactive clone (Lyar), (10ug) |
CNY 3,710.00 |
|
| MR222894L3 | Lenti ORF clone of Lyar (Myc-DDK-tagged) - Mouse Ly1 antibody reactive clone (Lyar) |
CNY 5,230.00 |
|
| MR222894L4 | Lenti ORF clone of Lyar (mGFP-tagged) - Mouse Ly1 antibody reactive clone (Lyar) |
CNY 5,230.00 |
