Ptprb (NM_029928) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	3230402H02Rik; C130094E24; Ptpz; Rptpb; VE-PTP; Veptp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR222130 representing NM_029928 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAGGCATGGAGCCCTAACGGCCTTGTGGATAACACTGAGCGTCGTGCAGACTGGAGTGGCAGAGC AAGTGAAATGTAACTTCACACTGTTGGAGTCCAGGGTCTCTAGCTTGTCAGCGTCTATCCAGTGGAGGAC TTTCGCGTCACCCTGTAACTTTAGCCTCATCTACAGCAGTGATACCTCGGGGCCCATGTGGTGCCATCCT ATTCGGATAGACAACTTTACCTACGGATGTAACCCCAAGGATTTACAAGCAGGGACCGTCTATAACTTCA GGATTGTTTCTCTGGATGGAGAAGAGAGCACTCTGGTCTTACAGACAGATCCGTTGCCTCCTGCCAGGTT TGAAGTCAATCGGGAGAAAACAGCATCAACCACCCTGCAGGTCCGGTGGACTCCCTCTTCTGGAAAAGTC TCCTGGTATGAGGTGCAATTATTTGATCATAACAATCAAAAGATACAAGAAGTCCAAGTTCAAGAAAGTA CCACCTGGAGCCAATATACTTTTCTGAACCTCACTGAGGGTAACAGTTACAAAGTTGCCATCACAGCTGT TTCGGGAGAAAAGCGCTCCTTTCCGGTGTATATCAATGGCTCTACAGTACCATCTCCAGTGAAAGATCTT GGCATTTCCCCCAATCCTAATTCTCTCCTAATTTCCTGGTCTCGTGGTTCTGGGAATGTGGAACAATACA GGCTGGTGCTAATGGATAAAGGGGCCATCGTTCAAGACACAAACGTGGACAGGCGTGATACTTCTTATGC TTTTCACGAGCTGACCCCTGGCCACCTCTACAACCTCACTATTGTCACCATGGCCTCGGGACTGCAAAAC TCCAGGTGGAAACTGGTGAGGACCGCTCCCATGGAAGTCTCAAATCTGAAGGTGACAAATGACGGGAGGT TGACCTCTCTAAATGTGAAGTGGCAGAAACCCCCTGGGGATGTAGATTCCTACAGCATTACCCTGTCTCA CCAAGGGACCATCAAAGAATCCAAAACATTAGCACCTCCTGTTACTGAAACTCAATTTAAGGACTTAGTC CCTGGACGGCTTTACCAAGTGACCATCAGCTGCATCTCTGGTGAGCTCTCTGCTGAGAAGTCAGCAGCGG GGAGAACAGTTCCAGAAAAAGTGAGGAATCTGGTTTCCTACAACGAGATTTGGATGAAGTCCTTTACAGT GAACTGGACGCCCCCTGCTGGAGATTGGGAGCATTATCGTATCGTGCTCTTCAATGAATCCTTGGTCTTG CTCAACACCACAGTGGGAAAGGAAGAAACGCACTATGCCTTGGATGGCTTGGAGCTCATACCAGGAAGAC AGTATGAGATAGAAGTCATTGTTGAGAGCGGAAATCTGCGGAATTCCGAGCGCTGTCAAGGCAGGACAGT ACCCCTGGCTGTCCTCCAGCTTCGCGTCAAACACGCTAACGAAACTTCACTGGGCATCACGTGGCGGGCC CCTCTAGGCGAATGGGAGAAATACATCATTTCGTTGATGGACAGAGAGCTCTTGGTCATCCACAAGTCAC TCTCCAAAGATGCCAAAGAATTCACTTTTACAGACCTGATGCCTGGACGGAATTACAAGGCTACTGTCAC TAGCATGAGTGGAGATTTAAAACAGTCATCTTCAATCAAAGGAAGAACAGTGCCTGCCCAGGTGACTGAC CTGCACGTCAACAACCAAGGGATGACCAGTAGTCTGTTCACTAACTGGACAAAGGCACTGGGAGATGTAG AGTTCTACCAAGTTTTACTGATCCATGAAAATGTGGTTGTCAAGAACGAGAGTGTTTCCAGTGATACCAG CAGATACAGCTTCCGCGCCCTGAAACCCGGCAGCCTCTACTCCGTGGTGGTGACCACGGTGAGTGGAGGG ATCTCCTCCCGGCAGGTGGTGGCGGAAGGAAGAACAGTCCCGTCCAGCGTGAGTGGGGTGACAGTCAACA ATTCTGGCCGGAATGACTACCTCAGCGTTTCCTGGCTGCCGGCGCCTGGAGAAGTGGATCACTACGTGGT GAGCCTCTCCCACGAGGGCAAGGTGGATCAGTTCCTCATCATCGCCAAATCTGTCAGCGAGTGTTCCTTC AGCTCCCTCACCCCGGGCCGCCTCTACAACGTCACTGTAACCACCAAGAGCGGCAATTATGCAAGCCACT CCTTCACCGAGGAACGGACAGTGCCAGACAAGGTCCAGGGAATCAGTGTTAGCAACTCTGCCAGAAGCGA CTACTTAAAGGTGTCCTGGGTGCATGCCACTGGAGACTTTGACCACTATGAAGTCACCATCAAAAACAGA GAAAGCTTCATTCAAACCAAAACCATCCCCAAGTCAGAAAATGAGTGTGAATTTATTGAGCTGGTTCCTG GACGCCTGTACAGCGTCACTGTCAGTACAAAGAGTGGACAATATGAAGCCAGTGAACAGGGGACAGGGAG AACGATCCCAGAGCCTGTGAAGGATCTCACCCTTCTCAACAGGAGTACGGAGGATCTCCATGTGACTTGG TCAAGAGCCAATGGGGATGTTGATCAGTACGAGGTCCAGCTGCTCTTCAACGACATGAAAGTCTTCCCTC ATATTCACCTTGTGAACACAGCAACTGAGTATAAGTTCACGGCGCTCACGCCGGGGCGCCATTACAAAAT CCTCGTCCTGACCATCAGTGGCGATGTCCAGCAGTCAGCCTTCATTGAAGGCCTCACAGTTCCCAGCACT GTCAAAAACATTCACATTTCTGCCAATGGAGCCACGGATAGGCTGATGGTAACCTGGAGCCCTGGTGGCG GGGATGTGGACTCCTATGTGGTGTCTGCATTCAGACAGGACGAGAAGGTTGACTCTCAGACCATTCCCAA GCATGCCTCGGAGCACACGTTCCACAGGCTGGAGGCCGGAGCCAAGTACAGGATCGCCATTGTTTCTGTC AGTGGGTCCCTGAGAAACCAGATAGATGCGCTCGGACAGACAGTCCCAGCGTCTGTCCAGGGAGTCGTCG CAGCCAATGCATACAGCAGTAATTCCTTAACAGTAAGTTGGCAGAAAGCCCTTGGTGTGGCAGAAAGATA CGATATCCTGCTTCTAAACGAGAATGGGCTTCTTTTGAGCAACGTGTCAGAGCCAGCTACGGCAAGACAG CACAAATTTGAAGATCTAACGCCAGGCAAGAAATACAAGATGCAGATCCTGACTGTCAGCGGAGGCCTCT TCAGTAAAGAATCTCAGGCTGAAGGCCGAACAGTCCCAGCAGCTGTCACCAATCTGAGGATCACAGAGAA CTCCAGTAGATACCTGTCCTTCGGCTGGACCGCCTCGGAGGGTGAACTCAGCTGGTACAACATCTTCCTC TACAACCCAGACAGGACTCTTCAGGAGCGAGCTCAAGTTGACCCGCTAGTCCAGAGCTTCTCTTTCCAGA ACTTGCTACAAGGCAGAATGTACAAGATGGTGATTGTCACTCACAGTGGGGAGCTGTCCAATGAGTCATT TATATTCGGCAGAACAGTTCCTGCTGCCGTGAACCATCTCAAAGGCTCCCATCGGAACACGACAGACAGC CTGTGGTTCAGCTGGAGCCCAGCCTCCGGGGACTTTGACTTCTATGAGCTGATTCTTTACAATCCCAACG GCACGAAGAAGGAGAACTGGAAAGAAAAGGACGTGACAGAGTGGCGTTTCCAAGGTCTTGTTCCTGGAAG GAAATACACCCTGTATGTGGTGACTCACAGTGGGGACCTCAGCAATAAAGTCACAGGGGAGGGCAGAACA GCCCCAAGTCCTCCGAGTCTTTTGTCATTCGCTGATGTTGCAAACACCTCCTTGGCTATCACCTGGAAGG GACCCCCAGACTGGACAGATTACAATGACTTTGAGCTGCAGTGGTTCCCTGGAGATGCACTTACCATCTT CAACCCCTACAGCAGCAGAAAGTCAGAAGGACGCATTGTGTACGGGCTTCACCCAGGGAGGTCCTATCAA TTCAGTGTCAAGACTGTGAGCGGGGACTCCTGGAAAACCTACAGCAAACCAATTTCTGGGTCTGTGAGGA CAAAGCCAGACAAGATACAAAACCTGCATTGCCGCCCCCAGAACTCCACGGCCATTGCCTGCTCTTGGAT ACCTCCTGACTCCGACTTTGATGGCTACAGCATTGAGTGCCGAAAAATGGATACCCAAGAAATCGAGTTT TCCAGAAAGCTGGAGAAAGAAAAATCACTGCTCAACATCATGATGTTAGTACCTCATAAGAGGTACCTGG TGTCCATCAAGGTGCAGTCGGCCGGCATGACCAGTGAGGTGGTTGAAGATAGCACCATCACCATGATAGA CCGCCCGCCTCAACCGCCTCCACACATCCGTGTGAATGAAAAGGATGTGCTAATCAGCAAATCTTCCATC AACTTTACTGTCAACTGCAGCTGGTTCAGCGACACCAACGGAGCGGTTAAATACTTTGCTGTGGTGGTGA GAGAGGCCGACAGCATGGATGAGTTGAAGCCAGAACAGCAGCACCCTCTCCCTTCCTACCTGGAGTACAG ACACAACGCCTCCATCCGAGTCTACCAGACCAATTATTTTGCCAGCAAATGTGCTGAAAGTCCCGACAGC AGTTCTAAAAGTTTCAACATTAAGCTTGGAGCAGAGATGGACAGCCTCGGTGGCAAATGTGATCCCAGTC AGCAGAAATTCTGTGATGGACCGCTGAAGCCACACACCGCCTACAGAATCAGCATCCGGGCTTTTACACA GCTATTTGACGAGGACTTGAAAGAGTTCACCAAACCTCTCTACTCGGATACGTTCTTCTCTATGCCCATC ACCACAGAGTCAGAGCCCTTGTTTGGAGTTATTGAAGGTGTGAGTGCTGGCCTGTTTCTAATTGGCATGC TGGTGGCCCTTGTTGCCTTCTTCATCTGCAGACAGAAAGCTAGCCACAGCAGGGAAAGGCCATCTGCCCG GCTCAGCATTCGTAGGGACCGGCCTTTGTCTGTCCATCTGAATCTGGGCCAGAAAGGCAACCGGAAAACT TCTTGCCCCATAAAGATCAATCAGTTTGAAGGGCATTTCATGAAGCTGCAGGCAGACTCCAACTACCTTC TATCCAAGGAATATGAGGACTTAAAAGACGTGGGTAGAAGCCAGTCATGTGACATTGCCCTCTTGCCTGA GAATCGAGGGAAAAATCGATACAACAACATATTGCCTTATGATGCCTCAAGAGTGAAGCTCTGCAATGTC GATGACGACCCTTGCTCTGACTACATCAACGCCAGCTACATCCCCGGTAACAACTTCAGACGAGAATACA TCGCCACTCAGGGACCGCTTCCAGGCACCAAGGATGACTTCTGGAAGATGGCGTGGGAGCAGAACGTTCA CAACATCGTCATGGTGACCCAGTGTGTTGAAAAGGGCCGAGTGAAGTGTGACCATTACTGGCCAGCAGAC CAGGACCCCCTCTACTACGGTGATCTCATCCTACAGATGGTCTCGGAGTCCGTGCTCCCCGAGTGGACCA TCAGGGAGTTTAAGATATGCAGTGAAGAACAGTTGGATGCACACAGACTCATCCGTCACTTTCACTACAC GGTGTGGCCAGACCATGGGGTCCCAGAGACCACCCAGTCCCTGATCCAATTTGTGAGGACAGTCAGGGAC TACATCAACAGAAGCCCCGGGGCTGGGCCCACCGTAGTGCACTGCAGCGCTGGTGTGGGCAGAACAGGGA CGTTCGTTGCCCTGGACCGGATCCTCCAGCAGTTGGACTCTAAGGACTCCGTGGACATTTATGGGGCAGT GCATGACCTAAGACTCCACAGGGTTCACATGGTCCAGACCGAGTGTCAATATGTGTATCTGCATCAGTGT GTAAGAGACGTCCTCAGAGCAAAGAAACTGCGGAACGAGCAAGAGAACCCCCTGTTTCCGATTTATGAGA ATGTGAATCCAGAGTATCACAGAGATGCAATCTACTCGAGACAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR222130 representing NM_029928 Red=Cloning site Green=Tags(s) MLRHGALTALWITLSVVQTGVAEQVKCNFTLLESRVSSLSASIQWRTFASPCNFSLIYSSDTSGPMWCHP IRIDNFTYGCNPKDLQAGTVYNFRIVSLDGEESTLVLQTDPLPPARFEVNREKTASTTLQVRWTPSSGKV SWYEVQLFDHNNQKIQEVQVQESTTWSQYTFLNLTEGNSYKVAITAVSGEKRSFPVYINGSTVPSPVKDL GISPNPNSLLISWSRGSGNVEQYRLVLMDKGAIVQDTNVDRRDTSYAFHELTPGHLYNLTIVTMASGLQN SRWKLVRTAPMEVSNLKVTNDGRLTSLNVKWQKPPGDVDSYSITLSHQGTIKESKTLAPPVTETQFKDLV PGRLYQVTISCISGELSAEKSAAGRTVPEKVRNLVSYNEIWMKSFTVNWTPPAGDWEHYRIVLFNESLVL LNTTVGKEETHYALDGLELIPGRQYEIEVIVESGNLRNSERCQGRTVPLAVLQLRVKHANETSLGITWRA PLGEWEKYIISLMDRELLVIHKSLSKDAKEFTFTDLMPGRNYKATVTSMSGDLKQSSSIKGRTVPAQVTD LHVNNQGMTSSLFTNWTKALGDVEFYQVLLIHENVVVKNESVSSDTSRYSFRALKPGSLYSVVVTTVSGG ISSRQVVAEGRTVPSSVSGVTVNNSGRNDYLSVSWLPAPGEVDHYVVSLSHEGKVDQFLIIAKSVSECSF SSLTPGRLYNVTVTTKSGNYASHSFTEERTVPDKVQGISVSNSARSDYLKVSWVHATGDFDHYEVTIKNR ESFIQTKTIPKSENECEFIELVPGRLYSVTVSTKSGQYEASEQGTGRTIPEPVKDLTLLNRSTEDLHVTW SRANGDVDQYEVQLLFNDMKVFPHIHLVNTATEYKFTALTPGRHYKILVLTISGDVQQSAFIEGLTVPST VKNIHISANGATDRLMVTWSPGGGDVDSYVVSAFRQDEKVDSQTIPKHASEHTFHRLEAGAKYRIAIVSV SGSLRNQIDALGQTVPASVQGVVAANAYSSNSLTVSWQKALGVAERYDILLLNENGLLLSNVSEPATARQ HKFEDLTPGKKYKMQILTVSGGLFSKESQAEGRTVPAAVTNLRITENSSRYLSFGWTASEGELSWYNIFL YNPDRTLQERAQVDPLVQSFSFQNLLQGRMYKMVIVTHSGELSNESFIFGRTVPAAVNHLKGSHRNTTDS LWFSWSPASGDFDFYELILYNPNGTKKENWKEKDVTEWRFQGLVPGRKYTLYVVTHSGDLSNKVTGEGRT APSPPSLLSFADVANTSLAITWKGPPDWTDYNDFELQWFPGDALTIFNPYSSRKSEGRIVYGLHPGRSYQ FSVKTVSGDSWKTYSKPISGSVRTKPDKIQNLHCRPQNSTAIACSWIPPDSDFDGYSIECRKMDTQEIEF SRKLEKEKSLLNIMMLVPHKRYLVSIKVQSAGMTSEVVEDSTITMIDRPPQPPPHIRVNEKDVLISKSSI NFTVNCSWFSDTNGAVKYFAVVVREADSMDELKPEQQHPLPSYLEYRHNASIRVYQTNYFASKCAESPDS SSKSFNIKLGAEMDSLGGKCDPSQQKFCDGPLKPHTAYRISIRAFTQLFDEDLKEFTKPLYSDTFFSMPI TTESEPLFGVIEGVSAGLFLIGMLVALVAFFICRQKASHSRERPSARLSIRRDRPLSVHLNLGQKGNRKT SCPIKINQFEGHFMKLQADSNYLLSKEYEDLKDVGRSQSCDIALLPENRGKNRYNNILPYDASRVKLCNV DDDPCSDYINASYIPGNNFRREYIATQGPLPGTKDDFWKMAWEQNVHNIVMVTQCVEKGRVKCDHYWPAD QDPLYYGDLILQMVSESVLPEWTIREFKICSEEQLDAHRLIRHFHYTVWPDHGVPETTQSLIQFVRTVRD YINRSPGAGPTVVHCSAGVGRTGTFVALDRILQQLDSKDSVDIYGAVHDLRLHRVHMVQTECQYVYLHQC VRDVLRAKKLRNEQENPLFPIYENVNPEYHRDAIYSRH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_029928
ORF Size	5994 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_029928.2, NP_084204.2
RefSeq Size	11912 bp
RefSeq ORF	5997 bp
Locus ID	19263
UniProt ID	B2RU80
MW	224.9 kDa
Gene Summary	Plays an important role in blood vessel remodeling and angiogenesis. Not necessary for the initial formation of blood vessels, but is essential for their maintenance and remodeling. Can induce dephosphorylation of TEK/TIE2, CDH5/VE-cadherin and KDR/VEGFR-2. Regulates angiopoietin-TIE2 signaling in endothelial cells. Acts as a negative regulator of TIE2, and controls TIE2 driven endothelial cell proliferation, which in turn affects blood vessel remodeling during embryonic development and determines blood vessel size during perinatal growth. Essential for the maintenance of endothelial cell contact integrity and for the adhesive function of VE-cadherin in endothelial cells and this requires the presence of plakoglobin.[UniProtKB/Swiss-Prot Function]

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