Wdfy2 (NM_175546) Mouse Tagged ORF Clone
CAT#: MR217140
- TrueORF®
Wdfy2 (Myc-DDK-tagged) - Mouse WD repeat and FYVE domain containing 2 (Wdfy2)
ORF Plasmid: tGFP
"NM_175546" in other vectors (4)
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CNY 2,850.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 5830485M08; B130024L21Rik; ProF; ZFYVE22 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR217140 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGAGATCCAGCCCCAGCCACTGACCCGCAAGCCGATCCTGCTGCAGCGGGTCGAGGGGTCGC AGGAGGTGGTCAACATGGCGGTGATTGTACCTAAAGAAGAGGGTGTCATCAGCGTCTCGGAGGACAGGAC AGTTCGTGTTTGGTTAAAGAGGGACAGTGGACAGTACTGGCCAAGCATCTACCATGCAATGCCTTCTCCA TGTTCATGCATGTCTTTTAACCCGGAAACAAGAAGACTGTCCATAGGTCTAGACAATGGTACAATCTCAG AGTTTATTTTGTCTGAAGATTATAACAAGATGACACCTGTGAAAAACTATCAAGCACATCAGAGCAGAGT AACGATGGTCCTGTTTGTTCTGGAACTGGAGTGGGTGCTGAGCACAGGACAGGACAAGCAGTTCGCCTGG CACTGCTCTGAGAGTGGACAGCGCCTGGGAGGTTACCGCACCAGTGCTGTGGCCTCAGGCCTGCAATTTG ATGTTGAAACCCGTCATGTGTTTATTGGCGATCACTCGGGCCAAGTAACCATCCTCAAACTGGAACAAGA AAACTGCACACTGCTCACATCCTTCAGAGGGCACACAGGAGGGGTGACAGCACTCTGCTGGGACCCGGTG CAGCGCGTGCTATTCTCAGGCAGTTCCGATCACTCTGTCATCATGTGGGACATCGGTGGGAGAAAAGGCA CGGCCATCGAACTCCAAGGACACAATGACAAAGTCCAGGCCCTCTCCTATGCACAGCACACACGACAGCT CATCTCATGTGGCGGTGACGGTGGCATTGTCGTCTGGAACATGGATGTGGAAAGGCAGGAGACCCCTGAG TGGTTGGACAGTGACTCCTGCCAAAAGTGTGACCAGCCTTTCTTCTGGAACTTCAAGCAAATGTGGGACA GTAAGAAAATTGGACTAAGACAGCACCACTGCCGGAAGTGTGGGAAGGCCGTGTGCGGCAAGTGTAGCTC CAAGCGCTCTTCCATCCCACTCATGGGCTTTGAGTTTGAAGTGCGGGTCTGCGACAGCTGCCACGAGGCC ATCACAGATGAAGAACGCGCCCCCACAGCCACCTTCCATGACAGTAAGCATAACATTGTGCATGTGCATT TCGATGCAACCAGGGGATGGTTACTGACTTCTGGAACTGACAAGGTAATTAAGCTGTGGGACATGACCCC AGTGGTGTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217140 protein sequence
Red=Cloning site Green=Tags(s) MAAEIQPQPLTRKPILLQRVEGSQEVVNMAVIVPKEEGVISVSEDRTVRVWLKRDSGQYWPSIYHAMPSP CSCMSFNPETRRLSIGLDNGTISEFILSEDYNKMTPVKNYQAHQSRVTMVLFVLELEWVLSTGQDKQFAW HCSESGQRLGGYRTSAVASGLQFDVETRHVFIGDHSGQVTILKLEQENCTLLTSFRGHTGGVTALCWDPV QRVLFSGSSDHSVIMWDIGGRKGTAIELQGHNDKVQALSYAQHTRQLISCGGDGGIVVWNMDVERQETPE WLDSDSCQKCDQPFFWNFKQMWDSKKIGLRQHHCRKCGKAVCGKCSSKRSSIPLMGFEFEVRVCDSCHEA ITDEERAPTATFHDSKHNIVHVHFDATRGWLLTSGTDKVIKLWDMTPVVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_175546 |
ORF Size | 1203 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175546.4, NP_780755.2 |
RefSeq Size | 2011 bp |
RefSeq ORF | 1203 bp |
Locus ID | 268752 |
UniProt ID | Q8BUB4 |
MW | 45.1 kDa |
Gene Summary | Acts in an adapter protein-like fashion to mediate the interaction between the kinase PRKCZ and its substrate VAMP2 and increases the PRKCZ-dependent phosphorylation of VAMP2 (By similarity). Positively regulates adipocyte differentiation, by facilitating the phosphorylation and thus inactivation of the anti-adipogenetic transcription factor FOXO1 by the kinase AKT1 (PubMed:18388859). Plays a role in endosomal control of AKT2 signaling; required for insulin-stimulated AKT2 phosphorylation and glucose uptake and insulin-stimulated phosphorylation of AKT2 substrates (PubMed:20189988). Participates in transferrin receptor endocytosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC214269 | Wdfy2 (untagged) - Mouse WD repeat and FYVE domain containing 2 (Wdfy2), (10ug) |
CNY 3,990.00 |
|
MG217140 | Wdfy2 (tGFP-tagged) - Mouse WD repeat and FYVE domain containing 2 (Wdfy2), (10ug) |
CNY 3,140.00 |
|
MR217140L3 | Lenti ORF clone of Wdfy2 (Myc-DDK-tagged) - Mouse WD repeat and FYVE domain containing 2 (Wdfy2) |
CNY 4,750.00 |
|
MR217140L4 | Lenti ORF clone of Wdfy2 (mGFP-tagged) - Mouse WD repeat and FYVE domain containing 2 (Wdfy2) |
CNY 4,750.00 |