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Kif24 (NM_024241) Mouse Tagged ORF Clone
CAT＃: MR211920
- TrueORF®
Kif24 (Myc-DDK-tagged) - Mouse kinesin family member 24 (Kif24)
ORF Plasmid: tGFP
"NM_024241" in other vectors (4)
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CNY 9,280.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4933425J19Rik; 9430029L23Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211920 representing NM_024241
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCCTGGTTATATGAGTGTCTTTGTGAGGCTGAGCTTGCACAGTATTATCCTCATTTTACTGCGC TTGGCCTTCAAAAAATAGATGAATTAGCCAAGGTTACAATGAAGGACTACTCCAGATTGGGAGTCCATGA CATGAACGACCGCAAACGTCTCTTTCAGCTTATCAAAATCATTAAGATTATGCAGGAGGAAGATAAAGCC CTCGGTATCCCAGAGCATCCTCTTCAGGCAAGCAGCCTGTACACCAAGCCTCGGGAATTCAGATCTGGGC CCCGAAGACAACTGCATTTTGATTCTCCTTCTGCCAGCAAAGATAAAATGGCCAACAATGAAACAGGCAG TTTATCCAACTTCTCTGTGGATGAGCAGAAGTCCACTTACCTGAAAGTGCTGGAGCACATGCTACCAGAT GATTCCCAGTGCCAGACAAAAATAAGAGCCCCGGATGCCTCTGCTGCTGATGCTTCCATGCAAACGGAAA CCAACGCTCCGCTCTTTTCATCAAATTACTTCTCTCCACAACTGGGAAATTGTGATATTCCCGTTATTCA AAGAGTCTCTCATGTGTCAGGGTATAACTATGGAATTCCTCATTCTTGTGTCAGGCAGATCACCTCAGAG AATCCTTGGACTGAAATGGAGAAAATCAGAGTTTGTGTTCGAAAACGGCCTTTAGGTGTAAGGGAAGTAC GTCGTGGAGAAGTTAATGTTATTACTGTAGAAGATAAAGAAACTCTGCTCGTACATGAGAAAAAAGAAGC AGTTGACCTTACTCAATATATTCTTCAGCATGTTTTTTATTTTGATGAAGTCTTTGGTGAGGCATGCAGC AATCAAGATGTATACCTGAAGACTGCTCACCCCCTCATTCAGCATATTTTCAACGGAGGAAGTGCCACTT GCTTTGCATATGGACAAACAGGTGCGGGGAAAACCTATACCATGATAGGAACTCATCAAAACCCAGGATT GTATGCTCTGGCTGCCAAAGATATCTTCAGGCAGCTGAAAGTATCCCAGTCAAGAAGGAATCTCTTTGTG TGGATCAGCTTCTATGAGATCTACTGTGGACAACTTTATGACCTCCTAAATAGAAGAAAACGGCTCTTTG CAAGAGAAGATAGCAAGCATGTGGTACAGATAGCTGGCCTTCGAGAGCTCCAAGTGGACAGTGTGGAGCT ACTCCTACAGGTGATCTTAAAGGGCAGCAAGGAGCGCAGCACAGGGGCCACTGGTGTCAACGCAGACTCC TCCCGTTCCCATGCTATCATCCAAATTCAGATCAAAGATTCAGCCAAGAGGACATTTGGCAGGATCTCTT TCATTGACTTGGCTGGGAGTGAGAGAGCAGCAGATGCCAGGGACTCAGACAGACAGACAAAGATGGAAGG CGCGGAGATCAACCAGAGTCTTCTGGCTCTGAAAGAATGTATCCGAGCACTGGACCAGGAACACACCCAC ACGCCTTTCAGGCAGAGCAAACTGACTCAGGTCCTGAAGGACTCTTTCATTGGCAATGCCAAAACTTGCA TGATCGCCAACATCTCCCCAAGCCACATAGCCACAGAGCATACATTGAATACCTTGCGCTATGCTGACCG GGTGAAGGAACTAAAGAAAGGCGTTAAGTGTTGTGCTTCAGCTACCAGTCAGAATCAGACATCTGCAAAC GCCTCTCCAAAACGAATTCAGAGCTCCCCTGTCACCCTCCCCGGGGACAAGTGCTCCCCCAAAAAAGTTA AGCTGGGACTTCAGCAGTCACTTACTGTGGCCCCAGGCCCCACAAAAGTTAAAGCCCATCCTTTGGCCAG CCATGTTCCCAACGTCCCCTTTACCTCTGGACCTAAAACCCCTGGTAAAAAAAGTAGTTCCCGAGGGAGT CCCACTCCAGAGTGGGACATGAAGGCTAGCCCTCGCAAAGGAACTACGAGATCTGGCCATTCGATAAAAA AGGGAGCAGAGTCGGCACCACTGTGCTCTGAGAAAAGTCAAATTGGCAGCAAGATTGCTGTTGGGTGGGA GGGCAGGGCCTCAGACCCAGGAGAAGGTCTGCTGCGTGTCAGGCTGCCCACGAGAGGGAAGAAGGTGCAG CCAGTCCAGCCAGTGCAGAAGCAGCTCCTGTCCCGACCTCGGCTCCTTGCTAACAGCCACCACTTAGAAG CCACTCAGGACAGCAAGGTGGGCACACCTGCCGGGCTTGCCCCCGAAGCCTGGACAAACCCCATTCTACA GCAAAAGGAAAGGGAGGAGCATTTGCGATTCTACCACCAGCAGTTCCAGCAGCCACCGCTCCTCAAGCAG AAGCTAAATTACCAACCCCTGCAGAGGCTTTTATGCCAGCACAGGCCTTCAGAAGGTCGGCTCCAGAGTG AGACTGGCTTTCCCCTCCACTCTAACCCTGAGAACCGTGATGGAGCCCAGGCTGAGGACCTTGATGACAG TGACTTCAGTGAGGATTCTTTCTCGCACGGCTCCAGTCAGCCGGCCATGAAACAGGGGAGCACAGCCTTG GAAAGGAGCGGAAGTTCCTTCTTCCTTCATCAGGACAGGGAGCACAGCCCTGAGGAGCAGGCAGCAGAAA GGCAGCAATGTTTGCTTTTCAGCTCTGAGACGGATGGCAGTAAGAAGCGGCCAGCTGACAGCTGGGTGTA TTCCAGGGACCCCATCATCAGCCACAGAAGAGGAGCACTCAGTCAAAGCCACAGCCCAAGTATGGTATGC CCAGACTGGAGCAAGGAGGAAGACTCTGCCTCCTCAGGACCTTCTCCTAAGGACAACCGGGCCCAGAAAC CTTCCTCTTCACAGGTAGATTTTGTGCATCACCAAAAACCGGGTGAAGCTCAAGTCTCTGACATCAGACT GGAGGCCTTCACAAGTGAGGTTCCTGAGCAAGCTGAGGGCAGCTTGTCATCCCCATCCCCGGAAAATGGG CTGTCTTTTCCACTGTCCCATGTGGCTGTTTCTGGATCCCCAGACCAAAGAGACAGAGTCTGCACACCAT TAAGAGAAGTCAGTGAAAACAGAGTGACTCACACTCCAGGAAGAGTGAACAGCAGCACTCCTTTCCAAGA GGACTCTGGAGAGCAGATACAGATGTGTTCTGCAAATGCTTCTGGACTCATGGCTCCCCTCACTATGTCC CTCCTGGAGACCCCCTGTCATGAGGACCTTTCGTCCTTGGAGCAAATTGCCCAGGATGGAGCTGGCTATG GTTTCATGGCTGAGATTGTAGGAGGGCCAGCAGCAGGACACACAGTCCCATCTTATGATCAAGAGGCTGC TTTGCCAGTGTCTTCAGCTACTGAGTGCCTATGGCTATCTTCATCTCCCCCTGACAATCGGCCTAGTGGT GATCTTCCAGCTCTGTCCCCGTCACCCATCCACCAGCATTCACCTGACAAATTGCCCGGTAGAGAGGCCT ACCAGACCAGAAGGCCAATACTCTTGCCTGAGAATCATATGGGTAGTAAGCTATATGATGACCGTGCTGA AGAGACTGAACTGGGGGGCTCCCTCACATTCCCCAGAAAGCCGTCCTCCAACATACATGCCGGAGTACCC TATTCTACACCTTTCCTCACCTCATGTACAGGAAGTAGTAATGGAGTTGGCCGTCCCTGGGCCCAGGAGA GAAAACATCCTACAGGGGTTAGCTGCCAGGAGCTTGTTTCCTCTACAGATTCCAACAAGCCTCATTACAA CGAGGACATCGCATGGCTCAGGCACAGGCCAATCTCAAGGTGCTTAGATTCAGACTCTCCTGTGGTCCCC AGCTGTTCTTCCAAGGCCTTGAGGACATATTGTCCACTTACACCGGAGCAGGCACAGCAGGTGATTATCC GTGCACACAAAGAACAGCTGGATGAAATGGCTGAGCTGGACTTGAAGGAGGAGACCTTAATGACCCAGAT GGATTCTAATGATTTTGAAGATTTTGTGACCCAGCTGGATGAAATCATGGCTTTGAAGTCCAGGTGCATC CAAAGTCTGAGAAGCCAGCTACAGCTCTACCTCACCAGCCACAGGCCCGCTGCAGCCCCCGAAAGAACTG TGGTGTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211920 representing NM_024241
Red=Cloning site Green=Tags(s) MASWLYECLCEAELAQYYPHFTALGLQKIDELAKVTMKDYSRLGVHDMNDRKRLFQLIKIIKIMQEEDKA LGIPEHPLQASSLYTKPREFRSGPRRQLHFDSPSASKDKMANNETGSLSNFSVDEQKSTYLKVLEHMLPD DSQCQTKIRAPDASAADASMQTETNAPLFSSNYFSPQLGNCDIPVIQRVSHVSGYNYGIPHSCVRQITSE NPWTEMEKIRVCVRKRPLGVREVRRGEVNVITVEDKETLLVHEKKEAVDLTQYILQHVFYFDEVFGEACS NQDVYLKTAHPLIQHIFNGGSATCFAYGQTGAGKTYTMIGTHQNPGLYALAAKDIFRQLKVSQSRRNLFV WISFYEIYCGQLYDLLNRRKRLFAREDSKHVVQIAGLRELQVDSVELLLQVILKGSKERSTGATGVNADS SRSHAIIQIQIKDSAKRTFGRISFIDLAGSERAADARDSDRQTKMEGAEINQSLLALKECIRALDQEHTH TPFRQSKLTQVLKDSFIGNAKTCMIANISPSHIATEHTLNTLRYADRVKELKKGVKCCASATSQNQTSAN ASPKRIQSSPVTLPGDKCSPKKVKLGLQQSLTVAPGPTKVKAHPLASHVPNVPFTSGPKTPGKKSSSRGS PTPEWDMKASPRKGTTRSGHSIKKGAESAPLCSEKSQIGSKIAVGWEGRASDPGEGLLRVRLPTRGKKVQ PVQPVQKQLLSRPRLLANSHHLEATQDSKVGTPAGLAPEAWTNPILQQKEREEHLRFYHQQFQQPPLLKQ KLNYQPLQRLLCQHRPSEGRLQSETGFPLHSNPENRDGAQAEDLDDSDFSEDSFSHGSSQPAMKQGSTAL ERSGSSFFLHQDREHSPEEQAAERQQCLLFSSETDGSKKRPADSWVYSRDPIISHRRGALSQSHSPSMVC PDWSKEEDSASSGPSPKDNRAQKPSSSQVDFVHHQKPGEAQVSDIRLEAFTSEVPEQAEGSLSSPSPENG LSFPLSHVAVSGSPDQRDRVCTPLREVSENRVTHTPGRVNSSTPFQEDSGEQIQMCSANASGLMAPLTMS LLETPCHEDLSSLEQIAQDGAGYGFMAEIVGGPAAGHTVPSYDQEAALPVSSATECLWLSSSPPDNRPSG DLPALSPSPIHQHSPDKLPGREAYQTRRPILLPENHMGSKLYDDRAEETELGGSLTFPRKPSSNIHAGVP YSTPFLTSCTGSSNGVGRPWAQERKHPTGVSCQELVSSTDSNKPHYNEDIAWLRHRPISRCLDSDSPVVP SCSSKALRTYCPLTPEQAQQVIIRAHKEQLDEMAELDLKEETLMTQMDSNDFEDFVTQLDEIMALKSRCI QSLRSQLQLYLTSHRPAAAPERTVVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024241 |
| ORF Size | 4068 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024241.2, NP_077203.2 |
| RefSeq Size | 5616 bp |
| RefSeq ORF | 4071 bp |
| Locus ID | 109242 |
| UniProt ID | Q6NWW5 |
| MW | 150.2 kDa |
| Gene Summary | Microtubule-dependent motor protein that acts as a negative regulator of ciliogenesis by mediating recruitment of CCP110 to mother centriole in cycling cells, leading to restrict nucleation of cilia at centrioles. Mediates depolymerization of microtubules of centriolar origin, possibly to suppress aberrant cilia formation. Following activation by NEK2 involved in disassembly of primary cilium during G2/M phase but does not disassemble fully formed ciliary axonemes. As cilium assembly and disassembly is proposed to coexist in a dynamic equilibrium may suppress nascent cilium assembly and, potentially, ciliar re-assembly in cells that have already disassembled their cilia ensuring the completion of cilium removal in the later stages of the cell cycle (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224320 | Kif24 (untagged) - Mouse kinesin family member 24 (Kif24), (10ug) |
CNY 17,390.00 |
|
| MG211920 | Kif24 (tGFP-tagged) - Mouse kinesin family member 24 (Kif24) |
CNY 11,880.00 |
|
| MR211920L3 | Lenti ORF clone of Kif24 (Myc-DDK-tagged) - Mouse kinesin family member 24 (Kif24) |
CNY 12,730.00 |
|
| MR211920L4 | Lenti ORF clone of Kif24 (mGFP-tagged) - Mouse kinesin family member 24 (Kif24) |
CNY 12,730.00 |
