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Uba6 (NM_172712) Mouse Tagged ORF Clone
CAT＃: MR211543
- TrueORF®
Uba6 (Myc-DDK-tagged) - Mouse ubiquitin-like modifier activating enzyme 6 (Uba6)
ORF Plasmid: tGFP
"NM_172712" in other vectors (4)
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CNY 7,336.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4930542H01; 5730469D23Rik; AU021846; AW124799; E1-L2; Ube1l2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211543 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGTCCGAGCCGTTGGCTGTGCTTTCCTGTGAAGAGGCGTCCTGCTCCTCCTGGGGAGCCTGCG GTGCAAGTAAAAATTTACCTACCATGACAACAGAATCTTTGGAAATCGATGATGGATTATACAGTCGCCA GAGATATGTTCTTGGTGACACAGCAATGCAGAAGATGGCCAAGTCCTGTGTCTTCTTAAGTGGTATGGGT GGTCTTGGAGTGGAAATTGCAAAGAATCTTGTTCTTGCAGGGATTAAGGCCCTAACAATTCATGATACAA AAAAATGCCAAGCATGGGATCTAGGGACCAATTTCTTCCTGTGTGAAGATGATGTTGTTAATGAGAGAAA CAGGGCTGAAGCTGTACTTCATCGTATTGCAGAACTAAATCCATATGTTCAGGTCTCATCATCCTCTGCC CCTCTTGATGAAACCACAGACCTCTCTTTCTTAGAAAAATATCAGTGTGTAGTATTGACTGAAATAAAAC TGACATTACAAAAGAAGATCAACAATTTTTGCCATTCTCATTGCCCTCCAATTAAGTTCATTAGTGCAGA TGTACATGGAATTTGGTCCAGGTTGTTTTGTGATTTTGGTGATGAATTTGAAGTTTCAGATACAACAGGA GAAGAACCAAAAGAAATTTTCATTTCAAACATAACGCAAGCTAATCCAGGTATTGTCACTTGCCTTGAAA GTCATCCTCACAAGCTTGAGACAGGACAGTTCCTAACATTTCGAGAAATTCATGGAATGACAGGCTTAAA TGGATCTGTACAACAGATAACTGTCATATCACCATTTTCCTTCAGCATTGGTGATACTACAAAACTGGAC CCATATTTACATGGAGGCATAGCCGTGCAGGTGAAGACTCCTAAAACATTCTGCTTTGAACCCCTGGAGA GCCAGATAAAACATCCAAGGTGCCTTATTGCAGATTTTAGCAAACCTGAGGCACCTTTAGAGATTCATCT CGCTATGCTTGCCTTGGACCAGTTTCAGGAGAACTATAACCGTAAGCCAAATATCAGATGTCAGCAGGAT TCAGATGAACTATTGAAGCTAACAGTATCTATAAATGAAACCTTGGAAGAGAAGCCTGAAGTGAATGCTG ACATTGTGCACTGGCTTTCGTGGACTGCCCAAGGCTTTTTACCACCACTCGCTGCTGCAGTTGGAGGTGT TGCTAGCCAAGAAGTCTTAAAAGCTGTGACGGGGAAGTTCTCGCCCTTGTGTCAATGGTTGTACCTTGAA GCAGCAGATACTGTGGAATCCCTAGGCAATCCTGGGCATGAAGAGTTTCTCCCACGAGGAGATAGATATG ATGCCATACGTGCCTGCATTGGAAATACATTGTGTCAGAAGCTACAAAATTTGAATATCTTCTTAGTAGG ATGTGGTGCCATAGGCTGTGAAATGTTAAAAAATTTTGCATTACTGGGTGTTGGTACAGGCAGAGAGAAA GGAATGGTAACAGTTACAGATCCTGACTTGATAGAAAAATCCAATTTAAACAGACAGTTCCTGTTTCGTC CTCATCACATACAGAAACCTAAAAGCTATACTGCTGCTGAAGCAACTCTGAAAATAAATCCTCAATTAAA AATAGATGCACACCTGAACAAAGTTTGTCCAGCCACTGAGAGTATATACAGTGATGAATTTTACACTAAG CAAGATATCATTATTACAGCACTAGATAATGTGGAAGCCAGGAGATATGTAGACAGTCGCTGTTTAGCAA ATCTACGGCCTCTCTTAGATTCTGGAACAATGGGCACCAAGGGACATACTGAAATTATCGTACCTCAATT AACTGAATCTTATAATAGTCATCGAGACCCCCCCGAAGAGGAAATACCATTTTGCACTCTAAAGTCCTTT CCAGCAGCTATCGAACATACTATCCAGTGGGCAAGAGACAAGTTTGAAAGTTCCTTCTCCCACAAACCTT CACTGTTTAACAAGTTTTGGCAAGCATATCCCTCTGCAGAAGATGTCTTACAGAAGATACAAAATGGACA AAGTTTAGAAGGCTGTTTTCAAGTTATTAAGCTACTTAGCAGAAGACCTAGAATTTGGTCCCAGTGTGTA GAGTTAGCAAGACTAAAGTTTGAAAAATACTTTAACCATAAGGCTCTGCAGCTTCTTCACTGTTTTCCTC TTGAGACAAGACTAAAAGATGGCAGTTTGTTTTGGCAATCACCAAAAAGGCCGCCTTCTCCAATAAAATT TGATTTAAATGAACCTTTGCACCTCAGTTTTCTTCAGAGTGCTGCAAAACTATATGCTACAGTATATTGT ATTCCGTTTTCAGAAAAGGACTTATCAGTGAATAGCCTCATGGACATTCTTTCAGAAGTTAAAATTGAGG AATTCAAGCCTTCCAACAAGGTTGTTCAAACAGACGAAACTGCAAGGAAGCCCGACCATGTTCCTGTCTC CAGTGAAGATGAAAGGAATGCTGTTTTCCAACTAGAAGAGGCTCTTTCCTCTAACAAAGCTACCAAAAGT GACCTTCAGATGACAGTGCTTTCATTTGAAAAAGATGATGACCGTAATGGACACATAGATTTCATCACAG CTGCTTCCAACCTTCGAGCCAAAATGTATAGCATTGAACCAGCTGACCGTTTTAAAACTAAACGCATAGC TGGTAAAATTATACCTGCTATAGCAACATCTACTGCTGCAGTTTCTGGCTTGGTTGCTTTGGAGATGATC AAGGTAGCTGGTGGCTATCCCTTCGACGCTTACAAAAACTGTTTCCTTAATTTAGCCATTCCAATAATAG TGTTTACAGAGACATCTGAAGTAAGAAAAACTGAAATCAGAAATGGAATATCATTTACAATTTGGGACAG ATGGACTGTACATGGAAAAGAAGATTTCACCCTCTCAGATTTCATAAATGCTGTCAAAGAAAACTATGGA ATTGAGCCGACAATGGTGGTGCAGGGAGTCAAAATGCTTTATGTCCCTGTAATGCCAGGTCATGCAAAAA GGCTAAAGTTAACAATGCACAAACTTGTGAAGCCTTCCACTGAGAAGAAATATGTGGATCTTACTGTGTC ATTTGCCCCAGACGCTGATGGAGATGAAGATTTGCCCGGCCCTCCAGTGAGATACTACTTCAGTCATGAC ACTAATGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211543 protein sequence
Red=Cloning site Green=Tags(s) MERSEPLAVLSCEEASCSSWGACGASKNLPTMTTESLEIDDGLYSRQRYVLGDTAMQKMAKSCVFLSGMG GLGVEIAKNLVLAGIKALTIHDTKKCQAWDLGTNFFLCEDDVVNERNRAEAVLHRIAELNPYVQVSSSSA PLDETTDLSFLEKYQCVVLTEIKLTLQKKINNFCHSHCPPIKFISADVHGIWSRLFCDFGDEFEVSDTTG EEPKEIFISNITQANPGIVTCLESHPHKLETGQFLTFREIHGMTGLNGSVQQITVISPFSFSIGDTTKLD PYLHGGIAVQVKTPKTFCFEPLESQIKHPRCLIADFSKPEAPLEIHLAMLALDQFQENYNRKPNIRCQQD SDELLKLTVSINETLEEKPEVNADIVHWLSWTAQGFLPPLAAAVGGVASQEVLKAVTGKFSPLCQWLYLE AADTVESLGNPGHEEFLPRGDRYDAIRACIGNTLCQKLQNLNIFLVGCGAIGCEMLKNFALLGVGTGREK GMVTVTDPDLIEKSNLNRQFLFRPHHIQKPKSYTAAEATLKINPQLKIDAHLNKVCPATESIYSDEFYTK QDIIITALDNVEARRYVDSRCLANLRPLLDSGTMGTKGHTEIIVPQLTESYNSHRDPPEEEIPFCTLKSF PAAIEHTIQWARDKFESSFSHKPSLFNKFWQAYPSAEDVLQKIQNGQSLEGCFQVIKLLSRRPRIWSQCV ELARLKFEKYFNHKALQLLHCFPLETRLKDGSLFWQSPKRPPSPIKFDLNEPLHLSFLQSAAKLYATVYC IPFSEKDLSVNSLMDILSEVKIEEFKPSNKVVQTDETARKPDHVPVSSEDERNAVFQLEEALSSNKATKS DLQMTVLSFEKDDDRNGHIDFITAASNLRAKMYSIEPADRFKTKRIAGKIIPAIATSTAAVSGLVALEMI KVAGGYPFDAYKNCFLNLAIPIIVFTETSEVRKTEIRNGISFTIWDRWTVHGKEDFTLSDFINAVKENYG IEPTMVVQGVKMLYVPVMPGHAKRLKLTMHKLVKPSTEKKYVDLTVSFAPDADGDEDLPGPPVRYYFSHD TNE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172712 |
| ORF Size | 3162 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172712.2, NP_766300.1 |
| RefSeq Size | 5101 bp |
| RefSeq ORF | 3162 bp |
| Locus ID | 231380 |
| UniProt ID | Q8C7R4 |
| MW | 118 kDa |
| Gene Summary | Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34 (By similarity). Essential for embryonic development. Required for UBD/FAT10 conjugation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223347 | Uba6 (untagged) - Mouse ubiquitin-like modifier activating enzyme 6 (Uba6), (10ug) |
CNY 13,490.00 |
|
| MG211543 | Uba6 (tGFP-tagged) - Mouse ubiquitin-activating enzyme E1-like 2 (Ube1l2) |
CNY 9,220.00 |
|
| MR211543L3 | Lenti ORF clone of Uba6 (Myc-DDK-tagged) - Mouse ubiquitin-like modifier activating enzyme 6 (Uba6) |
CNY 10,260.00 |
|
| MR211543L4 | Lenti ORF clone of Uba6 (mGFP-tagged) - Mouse ubiquitin-like modifier activating enzyme 6 (Uba6) |
CNY 10,260.00 |
