Pign (BC021148) Mouse Tagged ORF Clone

CAT＃: MR210838

TrueORF^®

Pign (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class N (cDNA clone MGC:13757 IMAGE:4024793)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"BC021148" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,750.00

货期*

现货

规格

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Gm20308; PIG-N
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR210838 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGTTTTTTGCTTTGGGATTGCTTATCCATTTTGTGTTCTTCGCTTCCATCTTTGACATTTATT TTACATCTCCTTTGGTTCATGGAATGACTCCCCAGTTTACACCTTTGCCTCCTCCAGCAAAAAGATTAGT GCTATTTGTTGCTGATGGCCTGAGAGCAGATACTCTTTATGAATTAGATGAAGATGGAAACTCCAGAGCA CCTTTCATTAGGAATGTTATAATTCATGAAGGCAGCTGGGGAGTATCTCACACACGTGTGCCAACAGAAT CTCGGCCTGGCCATGTAGCCTTGATAGCTGGATTTTATGAGGATGTCAGTGCAGTTGCCAAAGGATGGAA AGAAAATCCTGTAGAATTTGACTCTCTTTTTAATGAAAGCAAGTACACGTGGAGCTGGGGGAGTCCAGAT ATCTTGCCCATGTTTGCCAAAGGTGCTAGTGGTGACCATGTCTATACATATAGTTACGATGCTCAAAGAG AAGATTTTGGTGCCCACGATGCCACAAAACTGGATACGTGGGTTTTTGATAAAGTTAAGGACTTCTTTGA TGCTGCAAGAAACAACCAATCTTTGTTCACAAAAGTAAATGAAGAAAAAGTAGTTTTTTTCTTACATTTA TTGGGAATAGATACAAATGGACATGCTCATCGACCATCGTCAAGGGAATATAAGGACAATATTAAAAAAG TTGATGATGGAGTGAAGGAAATCGTATCAATTTTCAAGCATTTTTATGGAGATGATGGGAAAACAGCATT TATCTTTACCTCTGACCATGGAATGACAGACTGGGGTTCCCATGGGGCTGGTCATCCGTCAGAGACTTTA ACTCCGTTCGTTACTTGGGGAGCTGGAATCAAATTTCCTCAAAATGTATCAGCTCAGCAATATGATGATG AGTTTTTAAAAGAATGGAGATTGGAAAACTGGAAAAGGAGAGATGTCAATCAGGCTGATATTGCACCTCT GATGGCATCCCTTATTGGAGTCCCCTTTCCTCTTAATTCGGTGGGAATACTTCCTGTGGGTTATCTTAAC AACACTGGTCTCTTCAAAGCAGAGAGCATGTTTACCAATGCAGTACAGATTCTTGAACAGTTCAAGGTGA AAATGACTCAGAAAAAAGAAGCTACTTTACCGTTTTTGTTTACACCATTTAAGTTGCTTTCTGATTCTCA ACAACTGGACATTTTAAGAAAAGCAAGATCTTACATAAAACAAGAAAAATTTGATGAAGTAGTGTCCCTC TGTGAGGAGCTGATTGACCTCGCCCTGAGAGGATTGTCCTATTATCATACTTATGACAGACTGTTTCTGG GCATCAATGTTGCAGTTGGTTTTGTGGGATGGATGTCTTATACTTCTTTGTTAATCATCAAGTCGCATTC TAATATCCCAAAAGGCACTAGGAAGGAAGGTAAGAAGCCACACTGTCTCCTGCTTTATAGCTTCATAGCT ACTGGTGTCTTAGTAGCATGCTTCCTGATGATCCAAGCCTGTCCCTGGACTTACTATGTGTACTGCTTGC TGCCAGTGCCTATATGGTATGCAGTTCTAAGAGAACATGAAGTTATTCAAGACCTGGTTGAATCACTATT GACTTTTCCTCGGAGCCATTTTGTTGCCTACTTGCTTGTCTTTACCTTGGGTATTGAAGTACTGGTGCTC AGTTTCTTCTACCGCTACATGTTGACAGCGGGACTGATTGTGTTTGCAGGCTGGCCGTTTCTCACTCAGC TGTGGACCCGAGCAAAGATCACCTTCCTGAGTTGGGCTTTCTTCTCTTTGCTTCTGGCAGTGTTCCCACT GATGCCTGTTGTGGGTAGAAAGCCAAACCTTTCTCTAGTAATGGGTGCTGGATTCCTGGTTCTTCTGCTG TCGCTGGCTGTTGTAACAACTCTCGGAAAAAGAAACATTAAATTGGTAAAGGGCGAGCTGTTGGTACTTC TGTTACAGATGCTGAGTACAGTGCTCTCTATGTATGTGGTGTATAGCACCCACCACAGTCTGTTAAAGAA AGAAGGCCTGCCTCTTATGAATCAGATTGTGAGCTGGGCAACATTAGCCTCTTCTCTGGTGGCACCACTC CTGAGCTCCACAGCTCTCTCTCAGCGACTGGCCAGCATACTCCTCTCCTTGATGTCCACCTACCTGCTCC TAAGCACTGGGTATGAAGCTCTCTTTCCTTTAGTGCTGTCCTGTTTGATGTTTGTCTGGATCCAAGTGGA ACAAGAGACCCTTCAACAGCCTGGTGTTTCTTGTAAACAGAAGCTCACCAGCATCCAGTTCACCTGTGAC ACTGATATAGCTCAGTTCCGACAGCTCTGTCCGGATGACATCCGTAGGGCCTTTTTCCTCATATTGTTGA TCTTTCCTGTATTCTTAAGACTATTCGACTACAAAAGAATAAGGATTCTGTTAGCCCAAGAAAATTATCA TAGGTCATTAAAGGCTGCCTGGGAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR210838 protein sequence Red=Cloning site Green=Tags(s) MLLFFALGLLIHFVFFASIFDIYFTSPLVHGMTPQFTPLPPPAKRLVLFVADGLRADTLYELDEDGNSRA PFIRNVIIHEGSWGVSHTRVPTESRPGHVALIAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPD ILPMFAKGASGDHVYTYSYDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLFTKVNEEKVVFFLHL LGIDTNGHAHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKTAFIFTSDHGMTDWGSHGAGHPSETL TPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWKRRDVNQADIAPLMASLIGVPFPLNSVGILPVGYLN NTGLFKAESMFTNAVQILEQFKVKMTQKKEATLPFLFTPFKLLSDSQQLDILRKARSYIKQEKFDEVVSL CEELIDLALRGLSYYHTYDRLFLGINVAVGFVGWMSYTSLLIIKSHSNIPKGTRKEGKKPHCLLLYSFIA TGVLVACFLMIQACPWTYYVYCLLPVPIWYAVLREHEVIQDLVESLLTFPRSHFVAYLLVFTLGIEVLVL SFFYRYMLTAGLIVFAGWPFLTQLWTRAKITFLSWAFFSLLLAVFPLMPVVGRKPNLSLVMGAGFLVLLL SLAVVTTLGKRNIKLVKGELLVLLLQMLSTVLSMYVVYSTHHSLLKKEGLPLMNQIVSWATLASSLVAPL LSSTALSQRLASILLSLMSTYLLLSTGYEALFPLVLSCLMFVWIQVEQETLQQPGVSCKQKLTSIQFTCD TDIAQFRQLCPDDIRRAFFLILLIFPVFLRLFDYKRIRILLAQENYHRSLKAAWEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	BC021148
ORF Size	2478 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC021148, AAH21148
RefSeq Size	4038 bp
RefSeq ORF	2480 bp
Locus ID	27392
MW	93.8 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC200937	Pign (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class N (cDNA clone MGC:13757 IMAGE:4024793), (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,000.00
MG210838	Pign (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class N (cDNA clone MGC:13757 IMAGE:4024793)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,230.00
MR210838L3	Lenti ORF clone of Pign (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class N (cDNA clone MGC:13757 IMAGE:4024793)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,650.00
MR210838L4	Lenti ORF clone of Pign (mGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class N (cDNA clone MGC:13757 IMAGE:4024793)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,650.00

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