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Plcd1 (NM_019676) Mouse Tagged ORF Clone
CAT＃: MR210488
- TrueORF®
Plcd1 (Myc-DDK-tagged) - Mouse phospholipase C, delta 1 (Plcd1)
ORF Plasmid: tGFP
"NM_019676" in other vectors (4)
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CNY 5,544.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW212592; C79986 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210488 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCCGGTCGGGACTTCCTGACCTTGCACGGGCTGCAGGATGACCCGGACCTTCAGGCCCTCCTGA AGGGCAGCCAGCTTCTGAAGGTGAAGTCCAGCTCGTGGCGTAGAGAACGCTTCTACAAGCTACAGGAGGA CTGCAAGACCATCTGGCAGGAATCTCGAAAGGTCATGAGGTCCCCGGAGTCGCAGCTGTTCTCCATCGAG GACATTCAGGAGGTGCGGATGGGACACCGCACAGAAGGCCTGGAGAAATTTGCCAGAGACATACCCGAGG ACCGATGCTTCTCCATCGTCTTCAAGGACCAGCGCAATACCCTAGACCTCATCGCCCCGTCTCCAGCTGA CGTCCAGCATTGGGTGCAGGGCCTGCGCAAGATCATCGACCGCTCGGGCTCCATGGACCAGCGGCAGAAG CTGCAGCACTGGATTCACTCCTGCTTGCGAAAGGCTGATAAAAACAAGGATAACAAGATGAACTTCAAGG AGGTGAAGGACTTCCTGAAGGAGCTCAACGTACAGGTGGATGACAGCTACGCCAGGAAGATCTTCAGGGA ATGTGACCACTCCCAGACAGACTCGCTGGAGGATGAGGAGATTGAGACCTTCTACAGGATGCTGACCCAG CGGGTAGAGATCGACCGTGCCTTTGCAGAGGCCGCAGGTTCTGCAGAGACCCTGTCGGTGGAGAAGCTAG TGACGTTCCTGCAGCATCAGCAGCGGGAGGAGGAGGCGGGGCCAGCCTTGGCCCTCTCTCTCATTGAGCG CTACGAGCCCAGTGAGACTGCCAAAGCCCAGCGGCAGATGACCAAGGATGGCTTCCTCATGTACCTGCTG TCTGCTGACGGTAACGCCTTCAGCCTGGCGCACCGCCGTGTCTACCAGGACATGAACCAGCCGCTCAGCC ACTACTTAGTGTCTTCTTCCCACAACACCTACCTACTGGAGGACCAGCTCACAGGGCCCAGCAGCACGGA GGCCTACATCCGGGCTCTGTGCAAAGGCTGCCGGTGTCTGGAGCTGGACTGCTGGGACGGCCCCAACCAG GAACCCATCATCTACCACGGCTACACTTTTACCTCTAAGATACTTTTCTGCGACGTGCTCAGGGCCATCC GGGACTACGCCTTCAAGGCATCTCCCTACCCAGTTATCCTGTCCCTGGAGAACCACTGCAGCCTGGAGCA ACAACGAGTCATGGCTCATCACCTGAGGGCCATCCTGGGGCCCATGCTGTTGGACCAGCCGCTGGATGGG GTTACCACGAGCCTGCCTTCGCCTGAGCAACTGAAGGAAAAGATTCTGTTGAAAGGGAAGAAGCTGGGCG GGCTGCTACCTGCTGGAGGGGAAAACGGGCCTGAAGCCACCGATGTGTCTGACGAAGATGAGGCTGCTGA AATGGAAGATGAGGCCGTGCGCAGCCAAGTGCAACACAAGCCCAAGGAGGATAAGCTAAAGCTGGTGCCG GAGCTCTCCGACATGGTCATTTACTGCAAGAGTGTCCACTTTGGTGGCTTCTCCAGTCCTAGCACCTCTG GGCAGGCATTCTATGAGATGGCTTCCTTCTCCGAGAGCCGTGCCCTGCGGCTGCTGCAAGAATCAGGAAA TAGTTTTGTCCGCCATAACGTGGGCCATCTGAGCAGGATCTACCCAGCTGGGTGGAGGACAGATTCCTCC AACTACAGTCCCGTGGAGATGTGGAATGGAGGCTGCCAGATTGTGGCTCTGAACTTCCAAACCCCTGGGC CAGAAATGGATGTTTACCTTGGCTGCTTCCAGGACAATGGAGGTTGTGGGTATGTGCTGAAACCGGCCTT CCTGAGAGACCCTGACACCACCTTCAACTCACGGGCCCTGACTCAGGGGCCCTGGTGGGCCCCAAAGAAG CTCAGAGTCTGGATCATCTCTGGACAGCAGCTGCCGAAGGTCAATAAGAATAAGAATTCTATCGTGGACC CCAAGGTGATAGTGGAGATCCACGGTGTGGGCCAGGATGTGGCCAGCCGTCAGACAGCAGTTATTACCAA TAATGGTTTCAACCCACGGTGGGACACGGAGTTTGAGTTTGTGGTAGCAGTGCCTGACCTTGCCCTTGTA CGTTTCATGGTAGAGGATTATGACTCCTCCTCTAAGAACGACTTTATCGGCCAGAGTACTATCCCCTGGA ACAGCCTCAAGCAGGGGTACCGCCATGTCCACCTCTTGTCTAAGAATGGAGACCTGCATCCATCAGCCAC TCTCTTTGTGAAGATCTCCATCCAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210488 protein sequence
Red=Cloning site Green=Tags(s) MDSGRDFLTLHGLQDDPDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRSPESQLFSIE DIQEVRMGHRTEGLEKFARDIPEDRCFSIVFKDQRNTLDLIAPSPADVQHWVQGLRKIIDRSGSMDQRQK LQHWIHSCLRKADKNKDNKMNFKEVKDFLKELNVQVDDSYARKIFRECDHSQTDSLEDEEIETFYRMLTQ RVEIDRAFAEAAGSAETLSVEKLVTFLQHQQREEEAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLL SADGNAFSLAHRRVYQDMNQPLSHYLVSSSHNTYLLEDQLTGPSSTEAYIRALCKGCRCLELDCWDGPNQ EPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCSLEQQRVMAHHLRAILGPMLLDQPLDG VTTSLPSPEQLKEKILLKGKKLGGLLPAGGENGPEATDVSDEDEAAEMEDEAVRSQVQHKPKEDKLKLVP ELSDMVIYCKSVHFGGFSSPSTSGQAFYEMASFSESRALRLLQESGNSFVRHNVGHLSRIYPAGWRTDSS NYSPVEMWNGGCQIVALNFQTPGPEMDVYLGCFQDNGGCGYVLKPAFLRDPDTTFNSRALTQGPWWAPKK LRVWIISGQQLPKVNKNKNSIVDPKVIVEIHGVGQDVASRQTAVITNNGFNPRWDTEFEFVVAVPDLALV RFMVEDYDSSSKNDFIGQSTIPWNSLKQGYRHVHLLSKNGDLHPSATLFVKISIQD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019676 |
| ORF Size | 2271 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019676.1, NM_019676.2, NM_019676.3, NP_062650.1 |
| RefSeq Size | 2657 bp |
| RefSeq ORF | 2271 bp |
| Locus ID | 18799 |
| UniProt ID | Q8R3B1 |
| MW | 85.9 kDa |
| Gene Summary | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203035 | Plcd1 (untagged) - Mouse phospholipase C, delta 1 (Plcd1), (10ug) |
CNY 2,000.00 |
|
| MG210488 | Plcd1 (tGFP-tagged) - Mouse phospholipase C, delta 1 (Plcd1) |
CNY 6,370.00 |
|
| MR210488L3 | Lenti ORF clone of Plcd1 (Myc-DDK-tagged) - Mouse phospholipase C, delta 1 (Plcd1) |
CNY 7,700.00 |
|
| MR210488L4 | Lenti ORF clone of Plcd1 (mGFP-tagged) - Mouse phospholipase C, delta 1 (Plcd1) |
CNY 7,700.00 |
