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Dach2 (NM_001142570) Mouse Tagged ORF Clone
CAT＃: MR209355
- TrueORF®
Dach2 (Myc-DDK-tagged) - Mouse dachshund 2 (Drosophila) (Dach2), transcript variant 1
"NM_001142570" in other vectors (3)
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CNY 4,520.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9430028N04Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209355 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTCTCTGCACCTCCGGTGATCTCTGCAACTTCCAGCAGCGCCGGCGTCCCGGGGGGTTTATTCC GGGCTGAACCCCTGTATTCGTCTCCAGGAGAGCCCCCTCGCCTAACCCCTAACATGATCAACAGTTTCAT GGCCAATAACCATAACGGCAGTGTCTTGGGTGGCGGGATTGGTGGTGGCAGCGGTGGCAGCAGCAACACC AACACAAACGAGTGCCGGATGGTCGACATGCACGGGGTGAAGGTAGCTTCATTCCTGATGGATGGCCAGG AACTGATCTGCCTGCCGCAAGTCTTTGACCTTTTCCTCAAGCACCTGGTGGGAGGGTTGCACACAGTGTA CACTAAGCTGAAGAGACTCGACATATCTCCGGTGGTGTGCACTGTAGAGCAAGTTCGAATCCTCCGTGGG CTGGGGGCCATCCAGCCAGGGGTGAATCGTTGCAAGCTCATCACCAGGAAAGACTTTGAAACTTTGTTCA CCGATTGCACCAACGCCAGTTCAAGGCCTGGCAGGCCCCCTAAGCGTTCTTTGGGAGTATTGCAAGATAA TGCCCGACTTCTGCCCCATGCAGTCCCAGGCCTCTTATCACCAGGACTGATCACTCCAACAGGTATCACA GCTGCAGCGATGGCTGAGGCCATGAAGCTCCAGAAGATGAAGCTTATGGCAATGAATACTCTTCAGGGTA ATGGAAGCCAAAATGGGACTGAGTCAGAGCCAGATGACCTTAATTCTACGACAGGTGGAAGTGAATCCTC CTGGGATAAAGATAAGATACAATCTCCACTTGCTGCTTCTGGACCTCAACATGGAATTGCTCATGCAGCA CTTGCTGGTCAGCCAGGCCTTGGAGGTGCTCCGACCCTTAATCCACTTCAGCAGAATCACCTGCTAAGCA ATCGTCTGGATCTCCCATTTATGATGATGCCTCATCCCCTACTTCCAGTCAGCTTACCTCCTGCATCAGT TGCCATGGCAATGAATCAGATGAATCATCTCAATACTATTGCCAACATGGCTGCTGCAGCCCAGATTCAC AGTCCACTCTCCAGAGCTGGTGCCTCTGTTATAAAGGAACGGATCCCAGAGAGTCCTTCTCCTGCTCCCT CTCTGGAAGAGAGTCATCGTCCTGGGAGCCAGACCTCCTCCCACCCAAGCAGCAGTGTGTCCAGCTCTCC CTCGCAGATGGATCATCATTCAGAGAGAATGGTTATGATGCCCAACAATCGAGAAGAGCTTATTGTTGAC CAAGATAATGGACAAAGCATAAAAAAATTCCAGAGGGATAATAAAGAAGAAGTACCAGCTCAAATCCCAG TCATGAAGTCACCCTTGGATAAGATCCAGTTGGCTCCTGGACAGGCATTGCATCCTGGATTCCCTGGACC ATTCATTTTTGCAGATAGTCTATCTTCTGTGGAGACTCTGTTGACCAACATTCAGGGTCTACTGAAAGTG GCTTTGGATAATGCTCGTATCCAGGAGAAGCAGATTCAGCAGGAAAAGAAGGAACTGCGAATAGAGCTCT TCAGAGAAAGAGAAATTAGAGAAAACCTGGAGCGACAACTTGCAGTTGAGCTTCAAAGCAGAAGTACAAT GCAAAAGCGTCTGAAAAAAGAGAAAAAGGCTAAGAGAAAACTTCAGGAAGCCTTGGAATTTGAATCAAAG CGCCGCGAGCAAGTGGAGCAAGCACTTAAACAAGCTACATCTGGTGACAGTGGACTGAGAATGTTAAAAG ATTCTGGAATTCCAGATATTGAAATAGAAAACAGTGGAACGCCGCATGACAGTGCTGCTATGCAAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209355 protein sequence
Red=Cloning site Green=Tags(s) MAVSAPPVISATSSSAGVPGGLFRAEPLYSSPGEPPRLTPNMINSFMANNHNGSVLGGGIGGGSGGSSNT NTNECRMVDMHGVKVASFLMDGQELICLPQVFDLFLKHLVGGLHTVYTKLKRLDISPVVCTVEQVRILRG LGAIQPGVNRCKLITRKDFETLFTDCTNASSRPGRPPKRSLGVLQDNARLLPHAVPGLLSPGLITPTGIT AAAMAEAMKLQKMKLMAMNTLQGNGSQNGTESEPDDLNSTTGGSESSWDKDKIQSPLAASGPQHGIAHAA LAGQPGLGGAPTLNPLQQNHLLSNRLDLPFMMMPHPLLPVSLPPASVAMAMNQMNHLNTIANMAAAAQIH SPLSRAGASVIKERIPESPSPAPSLEESHRPGSQTSSHPSSSVSSSPSQMDHHSERMVMMPNNREELIVD QDNGQSIKKFQRDNKEEVPAQIPVMKSPLDKIQLAPGQALHPGFPGPFIFADSLSSVETLLTNIQGLLKV ALDNARIQEKQIQQEKKELRIELFREREIRENLERQLAVELQSRSTMQKRLKKEKKAKRKLQEALEFESK RREQVEQALKQATSGDSGLRMLKDSGIPDIEIENSGTPHDSAAMQA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001142570 |
| ORF Size | 1821 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001142570.1, NP_001136042.1 |
| RefSeq Size | 5991 bp |
| RefSeq ORF | 1821 bp |
| Locus ID | 93837 |
| UniProt ID | Q925Q8 |
| MW | 65.3 kDa |
| Gene Summary | Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression. Probably binds to DNA via its DACHbox-N domain.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219643 | Dach2 (untagged) - Mouse dachshund 2 (Drosophila) (Dach2), transcript variant 1, (10ug) |
CNY 7,790.00 |
|
| MR209355L3 | Lenti ORF clone of Dach2 (Myc-DDK-tagged) - Mouse dachshund 2 (Drosophila) (Dach2), transcript variant 1 |
CNY 6,460.00 |
|
| MR209355L4 | Lenti ORF clone of Dach2 (mGFP-tagged) - Mouse dachshund 2 (Drosophila) (Dach2), transcript variant 1 |
CNY 6,460.00 |
