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Dcp1a (NM_133761) Mouse Tagged ORF Clone
CAT＃: MR209319
- TrueORF®
Dcp1a (Myc-DDK-tagged) - Mouse DCP1 decapping enzyme homolog A (S. cerevisiae) (Dcp1a)
ORF Plasmid: tGFP
"NM_133761" in other vectors (4)
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CNY 4,480.00
CNY 5,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110066A22Rik; 4930568L04Rik; AU019772; D14Ertd817e; Mitc1; SMIF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209319 representing NM_133761
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACTTTCCTGTTCCACAGTCCGCCCCCGCCGGAGGGGCTCGGCTCTGAGATCCAAGATGGAGTTGT TGAGTCGAGCTGAGCAAGAGATGAGTCTGGCGGCCTTGAAACAACACGACCCCTATATCACCAGCATCGC AGACCTCACGGGCCAGGTCGCCCTGTACACTTTCTGCCCCAAAGCCAACCAGTGGGAGAAGACTGATATA GAAGGGACGTTATTTGTATACCGAAGGTCAGCTTCGCCTTACCATGGCTTTACCATTGTCAACCGACTGA ACATGCACAATCTGGTTGAGCCAGTGAATAAAGATTTGGAATTTCAGCTCCATGAACCATTTCTTCTGTA TAGAAATGCAAGTTTGTCAATATACAGTATCTGGTTCTATGACAAGAATGACTGTCATCGCATAGCAAAA CTCATGGCTGATGTGGTAGAAGAGGAGACACGGCGATCCCAGCAGGCTGCTCGAGATAAACAGAGTCCCA GCCAGGCCAATGGCTGCAGCGACCAGAGGCCCATAGATATCCTGGAGATGCTGAGCCGGGCCAAGGATGA GTATGAGAGGAATCAGATGGGCGGCTCAAATATCTCCAGTCCTGGGCTACAGCCAAGCACTCAGCTCTCT AATCTGGGAAGCACCGAGACTCTAGAAGAGACACCCTCTGGGTCACAGGATAAGTCTGCTCCGTCTGGTC ATAAACATCTGACAGTAGAAGAGTTATTTGGAACCTCCTTGCCAAAGGAACAACCAACAGCTATGGGTCT AGAGTCAGAGGACACAGACAAGCTGCTGGGAGATGCATCACAGAAAGAGCCCAGCTCATTCCTCCCATTT CCCTTTGAGCAGTCAGGGGGAGCCCCTCAGTCTGAAAACCTGGGTATCCATTCTGCCGCTCACCACACAG TTCAGCCTGAAGTCAGCACCCCAGTGCTGATCACTCCCGCCTCCATTGCACAGTCGGGTGACAAACATCC CCCAAGCTACACACTCCCACTGAGCCCTGTCCTCAGCCCCACTCTGCCAGCTGAAGCTCCTACCACACAA GTTCCCCACTTACCTCGGAATAGCACCATGATACAAGCAGTAAAGACCACACCCAGGCAGAAATCTCCAC TCCTGAACCAGCCAGTCCCTGAGCTAAGCCACAGCAGTCTGGTTGCTAGCCAGAGCCCCTTCAGAGCCCC GGTGAGCCTAGCAAACCCAGCTGGCACAGCCCTCCCAAGCGTTGATCTGCTTCAGAAACTCAGGTTGACC CCACAGCATGACCAAATACAAGCACAGCCGCTTGGAAAAGGAACCATGGCGCCCAGCTTTTCTTCGGCAG CTGGCCAACTGGCCACCCCTGAGAGCTTCATAGAGCCTTCTTCTAAGACAGCAGCAGCAAGAGCAGCAGT CTCAGCCTCCCTGAGCAACATGGTGCTTGCTCCAACCCTCCAGTCTATGCAGCAAAACCAGGATCCTGAA GTATTTTCCCAGCCTAAGGTGTTACCCAGTGCCATCCCGATTGCAGGCTCTCCATTGGTTCCTGCAACCA CCACAGCAGTATCTTCAGTCCTGCTGTCCCCCAGTGTGTTCCAGCAAACGGTTCCCAGGGCCGCAGACCT AGAGAGGAAAGCCAGCTCCCCCTCTCCCCTCACTGTTGGGACAGCAGAGAGTCAGAGAAAGCCTTCCATT ATCCTCAGCAAGTCTCAGCTCCAGGACACACTGATACACCTAATAAAGAATGACTCCAGCTTCCTCAGTA CGCTTCATGCAGTTTACTTGCAGGTTCTGACCAAGAACAAAGACAACCACAACCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209319 representing NM_133761
Red=Cloning site Green=Tags(s) MALSCSTVRPRRRGSALRSKMELLSRAEQEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDI EGTLFVYRRSASPYHGFTIVNRLNMHNLVEPVNKDLEFQLHEPFLLYRNASLSIYSIWFYDKNDCHRIAK LMADVVEEETRRSQQAARDKQSPSQANGCSDQRPIDILEMLSRAKDEYERNQMGGSNISSPGLQPSTQLS NLGSTETLEETPSGSQDKSAPSGHKHLTVEELFGTSLPKEQPTAMGLESEDTDKLLGDASQKEPSSFLPF PFEQSGGAPQSENLGIHSAAHHTVQPEVSTPVLITPASIAQSGDKHPPSYTLPLSPVLSPTLPAEAPTTQ VPHLPRNSTMIQAVKTTPRQKSPLLNQPVPELSHSSLVASQSPFRAPVSLANPAGTALPSVDLLQKLRLT PQHDQIQAQPLGKGTMAPSFSSAAGQLATPESFIEPSSKTAAARAAVSASLSNMVLAPTLQSMQQNQDPE VFSQPKVLPSAIPIAGSPLVPATTTAVSSVLLSPSVFQQTVPRAADLERKASSPSPLTVGTAESQRKPSI ILSKSQLQDTLIHLIKNDSSFLSTLHAVYLQVLTKNKDNHNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133761 |
| ORF Size | 1806 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133761.3, NP_598522.3 |
| RefSeq Size | 5616 bp |
| RefSeq ORF | 1809 bp |
| Locus ID | 75901 |
| UniProt ID | Q91YD3 |
| MW | 65.7 kDa |
| Gene Summary | Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201914 | Dcp1a (untagged) - Mouse DCP1 decapping enzyme homolog A (S. cerevisiae) (Dcp1a), (10ug) |
CNY 2,000.00 |
|
| MG209319 | Dcp1a (tGFP-tagged) - Mouse decapping enzyme (Dcp1a) |
CNY 5,800.00 |
|
| MR209319L3 | Lenti ORF clone of Dcp1a (Myc-DDK-tagged) - Mouse DCP1 decapping enzyme homolog A (S. cerevisiae) (Dcp1a) |
CNY 7,130.00 |
|
| MR209319L4 | Lenti ORF clone of Dcp1a (mGFP-tagged) - Mouse DCP1 decapping enzyme homolog A (S. cerevisiae) (Dcp1a) |
CNY 7,130.00 |
