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Csk (NM_007783) Mouse Tagged ORF Clone
CAT＃: MR207189
- TrueORF®
Csk (Myc-DDK-tagged) - Mouse c-src tyrosine kinase (Csk)
ORF Plasmid: tGFP
"NM_007783" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW212630; p50CSK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207189 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCAATACAGGCCGCCTGGCCATCCGGTACAGAATGTATTGCCAAGTACAACTTCCATGGCACTG CTGAGCAAGACCTTCCCTTCTGCAAAGGAGATGTGCTCACCATCGTGGCTGTCACCAAGGACCCCAACTG GTACAAAGCCAAAAACAAAGTGGGCCGTGAGGGCATCATCCCAGCCAACTATGTCCAGAAGCGTGAGGGT GTGAAGGCAGGCACCAAACTCAGCCTTATGCCCTGGTTCCACGGCAAGATCACACGGGAGCAGGCCGAGC GGCTTCTTTACCCACCAGAGACAGGCCTGTTCCTCGTGCGGGAAAGCACCAACTACCCTGGAGACTACAC ACTGTGTGTGAGCTGTGAGGGCAAGGTGGAGCACTACCGCATCATGTATCATGCGAGCAAGCTGAGCATT GATGAGGAGGTGTACTTTGAGAACCTCATGCAGCTGGTGGAGCACTACACCACAGATGCCGATGGACTCT GCACTCGCCTCATCAAACCAAAGGTCATGGAGGGCACCGTGGCGGCCCAGGATGAGTTCTACCGCAGTGG CTGGGCACTGAACATGAAGGAACTGAAGCTGCTACAGACAATAGGGAAGGGGGAGTTTGGAGATGTGATG CTGGGGGATTACCGGGGCAACAAAGTTGCAGTCAAGTGCATCAAGAATGACGCAACTGCCCAGGCCTTCC TGGCTGAAGCCTCCGTCATGACGCAACTTCGGCACAGCAACCTCGTCCAGCTGCTGGGTGTGATTGTGGA GGAGAAGGGTGGGCTCTACATCGTCACAGAGTACATGGCCAAGGGGAGTTTGGTGGACTATCTTCGATCA CGTGGTCGTTCGGTGCTAGGTGGAGACTGTCTCCTCAAATTCTCATTAGACGTCTGTGAAGCCATGGAGT ACCTGGAGGGTAACAATTTTGTGCACCGGGACTTGGCTGCCCGGAATGTGCTGGTGTCTGAAGACAACGT GGCCAAAGTCAGTGACTTTGGCCTCACTAAGGAAGCCTCCAGCACTCAGGACACAGGCAAGCTGCCAGTC AAATGGACAGCGCCTGAAGCCTTGAGAGAGAAGAAATTTTCCACCAAGTCTGATGTGTGGAGTTTCGGAA TCCTTCTCTGGGAAATCTATTCCTTCGGGCGAGTGCCTTACCCAAGAATTCCCCTGAAGGACGTCGTCCC TCGGGTGGAAAAGGGCTATAAGATGGACGCTCCGGATGGCTGCCCGCCCGCAGTCTACGAGGTGATGAAG AACTGCTGGCACCTGGATGCTGCCACACGGCCCACGTTTTTGCAGCTTCGGGAACAGCTCGAGCACATCA AGACCCATGAGCTGCACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207189 protein sequence
Red=Cloning site Green=Tags(s) MSAIQAAWPSGTECIAKYNFHGTAEQDLPFCKGDVLTIVAVTKDPNWYKAKNKVGREGIIPANYVQKREG VKAGTKLSLMPWFHGKITREQAERLLYPPETGLFLVRESTNYPGDYTLCVSCEGKVEHYRIMYHASKLSI DEEVYFENLMQLVEHYTTDADGLCTRLIKPKVMEGTVAAQDEFYRSGWALNMKELKLLQTIGKGEFGDVM LGDYRGNKVAVKCIKNDATAQAFLAEASVMTQLRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSLVDYLRS RGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFGLTKEASSTQDTGKLPV KWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYEVMK NCWHLDAATRPTFLQLREQLEHIKTHELHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007783 |
| ORF Size | 1353 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007783.3 |
| RefSeq Size | 2292 bp |
| RefSeq ORF | 1353 bp |
| Locus ID | 12988 |
| UniProt ID | P41241 |
| MW | 50.7 kDa |
| Gene Summary | Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN, CSK or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203625 | Csk (untagged) - Mouse c-src tyrosine kinase (Csk), (10ug) |
CNY 5,488.00 |
|
| MG207189 | Csk (tGFP-tagged) - Mouse c-src tyrosine kinase (Csk) |
CNY 7,088.00 |
|
| MR207189L3 | Lenti ORF clone of Csk (Myc-DDK-tagged) - Mouse c-src tyrosine kinase (Csk) |
CNY 6,370.00 |
|
| MR207189L4 | Lenti ORF clone of Csk (mGFP-tagged) - Mouse c-src tyrosine kinase (Csk) |
CNY 6,370.00 |
