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Lcat (NM_008490) Mouse Tagged ORF Clone
CAT＃: MR206988
- TrueORF®
Lcat (Myc-DDK-tagged) - Mouse lecithin cholesterol acyltransferase (Lcat)
ORF Plasmid: tGFP
"NM_008490" in other vectors (4)
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CNY 3,656.00
CNY 3,900.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI046659; D8Wsu61e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206988 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTGCCTGGCTCCCCATGGCAGCGGGTGCTGTTGCTGTTGGGGCTACTGCTCCCTCCTGCCACCC CCTTCTGGCTCCTCAATGTGCTCTTCCCCCCGCACACCACGCCCAAGGCTGAACTCAGTAACCACACACG GCCTGTCATCCTCGTGCCTGGCTGCTTGGGGAATCGGCTAGAAGCCAAGCTGGATAAACCAGATGTGGTG AACTGGATGTGCTACCGTAAGACAGAGGACTTCTTCACCATCTGGCTGGATTTCAACTTGTTTCTCCCCC TCGGGGTGGACTGCTGGATTGATAATACCAGGATTGTCTACAACCACAGCTCTGGGCGCGTGTCCAATGC CCCTGGTGTCCAGATCCGTGTCCCTGGCTTTGGCAAGACCGAATCTGTTGAGTACGTGGATGACAACAAG CTAGCAGGCTACCTGCACACACTGGTGCAGAATCTGGTTAACAACGGATATGTGCGGGATGAGACAGTGC GGGCCGCACCCTATGACTGGCGTCTGGCACCCCACCAGCAGGATGAATACTACAAGAAGCTGGCTGGCCT GGTAGAGGAGATGTATGCCGCTTATGGGAAGCCTGTCTTCCTCATTGGGCATAGCCTTGGCTGTCTGCAT GTGCTCCACTTCTTACTGCGGCAGCCTCAGTCCTGGAAGGACCACTTCATTGATGGTTTTATCTCTCTCG GGGCTCCGTGGGGTGGTTCCATCAAGGCCATGCGGATCCTGGCCTCAGGTGACAACCAGGGCATCCCCAT CCTGTCCAACATAAAGCTGAAAGAGGAGCAGCGCATAACCACGACTTCCCCCTGGATGCTCCCAGCCCCT CACGTGTGGCCTGAAGACCATGTGTTCATTTCCACACCAAACTTCAACTACACCGTCCAAGACTTTGAGC GTTTTTTCACAGATCTGCATTTTGAAGAAGGCTGGCACATGTTTCTTCAGTCTCGTGACCTACTGGAGCG CCTCCCCGCACCTGGTGTAGAAGTATATTGTCTCTACGGTGTGGGCAGACCCACACCCCACACCTACATC TATGACCACAACTTCCCCTACAAAGACCCCGTGGCTGCACTCTATGAAGATGGGGACGACACCGTAGCCA CCCGCAGCACTGAGCTCTGTGGCCAGTGGCAGGGCCGCCAGTCGCAGCCCGTACATTTGCTGCCCATGAA CGAGACAGATCACCTCAACATGGTCTTCAGCAATAAGACACTGGAGCATATCAATGCCATCCTACTGGGT GCCTACCGCACTCCTAAGTCGCCAGCTGCCAGCCCAAGTCCCCCACCCCCTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206988 protein sequence
Red=Cloning site Green=Tags(s) MGLPGSPWQRVLLLLGLLLPPATPFWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNRLEAKLDKPDVV NWMCYRKTEDFFTIWLDFNLFLPLGVDCWIDNTRIVYNHSSGRVSNAPGVQIRVPGFGKTESVEYVDDNK LAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLAPHQQDEYYKKLAGLVEEMYAAYGKPVFLIGHSLGCLH VLHFLLRQPQSWKDHFIDGFISLGAPWGGSIKAMRILASGDNQGIPILSNIKLKEEQRITTTSPWMLPAP HVWPEDHVFISTPNFNYTVQDFERFFTDLHFEEGWHMFLQSRDLLERLPAPGVEVYCLYGVGRPTPHTYI YDHNFPYKDPVAALYEDGDDTVATRSTELCGQWQGRQSQPVHLLPMNETDHLNMVFSNKTLEHINAILLG AYRTPKSPAASPSPPPPE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008490 |
| ORF Size | 1317 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008490.2 |
| RefSeq Size | 1370 bp |
| RefSeq ORF | 1317 bp |
| Locus ID | 16816 |
| UniProt ID | P16301 |
| MW | 49.7 kDa |
| Gene Summary | Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs) (PubMed:19065001). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels (PubMed:19065001). Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins (PubMed:19065001). Required for remodeling high-density lipoprotein particles into their spherical forms (PubMed:19065001). Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines (PubMed:8820107).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201471 | Lcat (untagged) - Mouse lecithin cholesterol acyltransferase (Lcat), (10ug) |
CNY 2,000.00 |
|
| MG206988 | Lcat (tGFP-tagged) - Mouse lecithin cholesterol acyltransferase (Lcat) |
CNY 4,280.00 |
|
| MR206988L3 | Lenti ORF clone of Lcat (Myc-DDK-tagged) - Mouse lecithin cholesterol acyltransferase (Lcat) |
CNY 5,800.00 |
|
| MR206988L4 | Lenti ORF clone of Lcat (mGFP-tagged) - Mouse lecithin cholesterol acyltransferase (Lcat) |
CNY 5,800.00 |
