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Jaml (NM_001005421) Mouse Tagged ORF Clone
CAT＃: MR205923
- TrueORF®
Amica1 (Myc-DDK-tagged) - Mouse adhesion molecule, interacts with CXADR antigen 1 (Amica1)
ORF Plasmid: tGFP
"NM_001005421" in other vectors (4)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AMICA; Amica1; Crea7; Gm638 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205923 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTTGCCTCCTGAAACTGATTGTGATTCCAGTAATCCTGGCCCCTGTAGGTTATCCACAGGGCCTGC CAGGCTTGACCGTTTCCTCCCCTCAGCTGAGAGTGCATGTGGGTGAATCAGTCTTGATGGGATGTGTTGT CCAGCGCACAGAAGAGAAACACGTGGACAGAGTGGATTGGCTCTTCTCGAAAGATAAAGATGATGCGAGT GAATATGTGCTGTTCTACTATTCCAACCTTAGCGTGCCTACGGGGCGCTTCCAGAACCGGTCACATTTGG TGGGGGACACCTTCCATAATGATGGTTCTCTCCTGCTCCAAGATGTTCAGAAGGCCGATGAGGGAATCTA CACCTGTGAAATCCGCCTCAAAAATGAGAGCATGGTGATGAAAAAGCCCGTGGAACTGTGGGTGCTACCA GAGGAACCTAAAGATCTCAGAGTCCGAGTAGGTGATACAACTCAGATGAGATGTTCTATCCAGAGCACAG AAGAGAAACGGGTGACCAAAGTAAACTGGATGTTTTCTTCAGGGAGCCATACTGAGGAGGAGACAGTCTT GAGCTATGACTCCAACATGCGTAGTGGAAAATTCCAGAGCCTGGGCCGCTTCCGCAACCGTGTAGACCTG ACAGGTGACATCTCCCGCAATGATGGCTCAATCAAACTTCAAACAGTGAAGGAGTCTGACCGAGGAATCT ACACTTGCAGCATCTACGTGGGAAAGCTGGAGTCCAGGAAAACCATTGTGCTGCATGTGGTCCAGGACGA ATTTCAAAGGACAATTTCACCAACTCCTCCAACTGATAAGGGTCAGCAGGGCATCCTGAATGGAAATCAG CTGGTGATCATTGTGGGGATCGTCTGTGCCACCTTCCTGCTGCTTCCGGTTTTGATATTAATTGTGAAGA AAGCCAAGTGGAATAAGAGCTCAGTAAGTTCTATGGCTTCTGTGAAGAGCCTGGAGAACAAAGAGAAGAT TAATCCAGAGAAGCATATCTACTCCTCCATAACTACGTGGGAGACGACAGAGAGAGGAATAAGTGGAGAG TCAGAGGGCACCTACATGACCATGAATCCAGTTTGGCCTTCTTCCCCCAAAGCATCCAGTTTGGTCCGCT CTTCAGTCAGATCCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205923 protein sequence
Red=Cloning site Green=Tags(s) MLCLLKLIVIPVILAPVGYPQGLPGLTVSSPQLRVHVGESVLMGCVVQRTEEKHVDRVDWLFSKDKDDAS EYVLFYYSNLSVPTGRFQNRSHLVGDTFHNDGSLLLQDVQKADEGIYTCEIRLKNESMVMKKPVELWVLP EEPKDLRVRVGDTTQMRCSIQSTEEKRVTKVNWMFSSGSHTEEETVLSYDSNMRSGKFQSLGRFRNRVDL TGDISRNDGSIKLQTVKESDRGIYTCSIYVGKLESRKTIVLHVVQDEFQRTISPTPPTDKGQQGILNGNQ LVIIVGIVCATFLLLPVLILIVKKAKWNKSSVSSMASVKSLENKEKINPEKHIYSSITTWETTERGISGE SEGTYMTMNPVWPSSPKASSLVRSSVRSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001005421 |
| ORF Size | 1140 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001005421.1 |
| RefSeq Size | 2472 bp |
| RefSeq ORF | 1140 bp |
| Locus ID | 270152 |
| UniProt ID | Q80UL9 |
| MW | 42.6 kDa |
| Gene Summary | Transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR, a plasma membrane receptor found on adjacent epithelial and endothelial cells. The interaction between both receptors mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. It also controls the transmigration of leukocytes within epithelial and endothelial tissues through adhesive interactions with epithelial and endothelial CXADR.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201873 | Amica1 (untagged) - Mouse adhesion molecule, interacts with CXADR antigen 1 (Amica1), (10ug) |
CNY 2,000.00 |
|
| MG205923 | Amica1 (tGFP-tagged) - Mouse adhesion molecule, interacts with CXADR antigen 1 (Amica1) |
CNY 3,140.00 |
|
| MR205923L3 | Lenti ORF clone of Amica1 (Myc-DDK-tagged) - Mouse adhesion molecule, interacts with CXADR antigen 1 (Amica1) |
CNY 4,750.00 |
|
| MR205923L4 | Lenti ORF clone of Amica1 (mGFP-tagged) - Mouse adhesion molecule, interacts with CXADR antigen 1 (Amica1) |
CNY 4,750.00 |
