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Aldob (NM_144903) Mouse Tagged ORF Clone
CAT＃: MR205585
- TrueORF®
Aldob (Myc-DDK-tagged) - Mouse aldolase B, fructose-bisphosphate (Aldob)
ORF Plasmid: tGFP
"NM_144903" in other vectors (5)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Al; Ald; Aldo-2; Aldo2; BC016435 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205585 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCACCGATTTCCAGCCCTCACCCCAGAGCAGAAGAAGGAGCTCTCTGAAATTGCCCAGCGCATTG TTGCCAATGGGAAGGGCATCCTGGCTGCAGATGAATCTGTGGGTACCATGGGAAACCGCCTGCAAAGGAT AAAGGTGGAGAACACCGAAGAGAACCGAAGGCAGTTCCGAGAACTCCTCTTTAGTGTGGACAATTCCATT AGCCAGAGCATCGGCGGAGTGATCCTTTTCCACGAGACCCTCTACCAGAAGGACAGCCAGGGAAATCTGT TCAGAAACGTTCTCAAGGAGAAGGGAATTGTGGTGGGCATCAAGTTGGACCAAGGAGGTGCCCCGCTTGC AGGAACAAACAAGGAAACCACCATTCAAGGGCTTGATGGCCTCTCTGAACGCTGTGCTCAGTACAAGAAA GATGGAGTTGACTTTGGGAAGTGGCGTGCTGTGTTGAGGATTGCTGACCAGTGTCCTTCCAGCCTTGCTA TCCAAGAAAATGCCAATGCTCTGGCTCGCTATGCCAGCATCTGTCAGCAGAATGGGCTGGTCCCTATTGT TGAGCCAGAGGTGCTTCCTGACGGAGACCATGACCTGGAGCACTGCCAGTATGTTTCTGAGAAGGTCCTG GCTGCTGTCTACAAGGCTCTCAATGATCATCATGTTTACCTAGAAGGCACCTTGCTAAAGCCAAACATGG TGACTGCTGGACATGCCTGCACGAAGAAGTATACACCAGAGCAGGTGGCTATGGCCACTGTCACAGCTCT CCACAGAACTGTTCCTGCAGCTGTTCCTGGTATCTGCTTTTTGTCTGGAGGTATGAGTGAGGAGGATGCT ACACTTAACCTCAATGCTATCAACCGTTGCCCTCTACCAAGGCCCTGGAAACTAAGCTTTTCATATGGAA GAGCCCTCCAGGCCAGTGCATTGGCTGCCTGGGGTGGCAAGGCTGCAAACAAGAAGGCAACCCAGGAAGC TTTCATGAAGCGGGCTATGGCTAACTGCCAGGCGGCCCAAGGACAGTACGTTCACACAGGCTCTTCAGGT GCTGCTGCCACCCAGTCGCTCTTCACAGCCTCCTACACCTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205585 protein sequence
Red=Cloning site Green=Tags(s) MAHRFPALTPEQKKELSEIAQRIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFRELLFSVDNSI SQSIGGVILFHETLYQKDSQGNLFRNVLKEKGIVVGIKLDQGGAPLAGTNKETTIQGLDGLSERCAQYKK DGVDFGKWRAVLRIADQCPSSLAIQENANALARYASICQQNGLVPIVEPEVLPDGDHDLEHCQYVSEKVL AAVYKALNDHHVYLEGTLLKPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEEDA TLNLNAINRCPLPRPWKLSFSYGRALQASALAAWGGKAANKKATQEAFMKRAMANCQAAQGQYVHTGSSG AAATQSLFTASYTY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144903 |
| ORF Size | 1095 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144903.3 |
| RefSeq Size | 1993 bp |
| RefSeq ORF | 1095 bp |
| Locus ID | 230163 |
| UniProt ID | Q91Y97 |
| MW | 39.5 kDa |
| Gene Summary | This gene encodes a subunit of the homotetrameric enzyme aldolase B, an isozyme of the class I fructose 1,6-bisphosphate aldolase enzyme. This enzyme catalyzes the conversion of fructose 1,6-bisphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Homozygous knockout mice for this gene exhibit liver damage and death following fructose ingestion. A pseudogene of this gene has been identified in the genome. [provided by RefSeq, Aug 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205585 | Aldob (tGFP-tagged) - Mouse aldolase 2, B isoform (Aldob) |
CNY 3,140.00 |
|
| MR205585L1 | Lenti ORF clone of Aldob (Myc-DDK-tagged) - Mouse aldolase B, fructose-bisphosphate (Aldob) |
CNY 4,750.00 |
|
| MR205585L2 | Lenti ORF clone of Aldob (mGFP-tagged) - Mouse aldolase B, fructose-bisphosphate (Aldob) |
CNY 4,750.00 |
|
| MR205585L3 | Lenti ORF clone of Aldob (Myc-DDK-tagged) - Mouse aldolase B, fructose-bisphosphate (Aldob) |
CNY 4,750.00 |
|
| MR205585L4 | Lenti ORF clone of Aldob (mGFP-tagged) - Mouse aldolase B, fructose-bisphosphate (Aldob) |
CNY 4,750.00 |
