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Aspa (NM_023113) Mouse Tagged ORF Clone
CAT＃: MR204432
- TrueORF®
Aspa (Myc-DDK-tagged) - Mouse aspartoacylase (Aspa)
ORF Plasmid: tGFP
"NM_023113" in other vectors (4)
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CNY 1,416.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Acy; Acy-; Acy-2; Acy2; nu; nur7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204432 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCTCTTGTGTTGCTAAAGAACCTATTAAGAAGATTGCCATCTTTGGAGGGACTCATGGAAATGAAC TGACCGGAGTGTTTCTAGTTACTCACTGGCTAAGGAATGGCACTGAAGTTCACAGAGCAGGGCTGGACGT GAAGCCATTCATTACCAATCCAAGGGCGGTGGAGAAGTGCACCAGATACATTGACTGTGACCTGAATCGT GTTTTTGACCTTGAAAATCTTAGCAAAGAGATGTCTGAAGACTTGCCATATGAAGTGAGAAGGGCTCAAG AAATAAATCATTTATTTGGTCCAAAAAATAGTGATGATGCCTATGACATTGTTTTTGACCTTCACAACAC CACTTCTAACATGGGTTGCACTCTTATTCTTGAGGATTCCAGGAATGACTTTTTAATTCAGATGTTTCAC TATATTAAGACTTGCATGGCTCCATTACCCTGCTCTGTTTATCTCATTGAGCATCCTTCACTCAAATATG CAACCACTCGTTCCATTGCCAAGTATCCTGTTGGTATAGAAGTTGGTCCTCAGCCTCACGGTGTCCTTAG AGCTGATATTTTAGACCAAATGAGAAAAATGATAAAACATGCTCTTGATTTTATACAGCATTTCAATGAA GGAAAAGAATTTCCTCCCTGTTCTATTGACGTCTATAAAATAATGGAGAAAGTTGATTATCCAAGGAATG AAAGTGGAGACATGGCTGCTGTTATTCATCCTAATCTGCAGGATCAAGACTGGAAACCATTGCACCCTGG AGATCCTGTGTTTGTGTCTCTTGATGGAAAAGTTATTCCACTGGGTGGAGACTGTACCGTGTACCCAGTG TTTGTGAATGAAGCTGCATATTATGAAAAAAAAGAAGCATTTGCAAAGACAACAAAACTAACACTCAGCG CAAAAAGCATCCGCTCCACTTTGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204432 protein sequence
Red=Cloning site Green=Tags(s) MTSCVAKEPIKKIAIFGGTHGNELTGVFLVTHWLRNGTEVHRAGLDVKPFITNPRAVEKCTRYIDCDLNR VFDLENLSKEMSEDLPYEVRRAQEINHLFGPKNSDDAYDIVFDLHNTTSNMGCTLILEDSRNDFLIQMFH YIKTCMAPLPCSVYLIEHPSLKYATTRSIAKYPVGIEVGPQPHGVLRADILDQMRKMIKHALDFIQHFNE GKEFPPCSIDVYKIMEKVDYPRNESGDMAAVIHPNLQDQDWKPLHPGDPVFVSLDGKVIPLGGDCTVYPV FVNEAAYYEKKEAFAKTTKLTLSAKSIRSTLH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023113 |
| ORF Size | 939 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023113.1, NM_023113.2, NM_023113.3, NM_023113.4, NM_023113.5, NP_075602.2 |
| RefSeq Size | 1537 bp |
| RefSeq ORF | 939 bp |
| Locus ID | 11484 |
| UniProt ID | Q8R3P0 |
| MW | 35.3 kDa |
| Gene Summary | This gene encodes an enzyme that deacteylates N-acetyl-L-aspartic acid (NAA) in the brain to yield acetate and L-aspartate. In humans, alterations in neuronal NAA concentration are associated with many neurodegenerative diseases (decrease associated with epilepsy, multiple sclerosis, myotrophic lateral sclerosis, and Alzheimer's disease; increase associated with Canavan disease). In mouse, mutations in this gene, which cause accumulation of NAA, result in demyelination and spongy degeneration in the CNS and serve as a pathophysiological model for Canavan disease. [provided by RefSeq, Dec 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201605 | Aspa (untagged) - Mouse aspartoacylase (Aspa), (10ug) |
CNY 2,000.00 |
|
| MG204432 | Aspa (tGFP-tagged) - Mouse aspartoacylase (aminoacylase) 2 (Aspa) |
CNY 2,850.00 |
|
| MR204432L3 | Lenti ORF clone of Aspa (Myc-DDK-tagged) - Mouse aspartoacylase (Aspa) |
CNY 4,750.00 |
|
| MR204432L4 | Lenti ORF clone of Aspa (mGFP-tagged) - Mouse aspartoacylase (Aspa) |
CNY 4,750.00 |
