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Echs1 (NM_053119) Mouse Tagged ORF Clone
CAT＃: MR203962
- TrueORF®
Echs1 (Myc-DDK-tagged) - Mouse enoyl Coenzyme A hydratase, short chain, 1, mitochondrial (Echs1), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_053119" in other vectors (4)
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CNY 2,081.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C80529 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203962 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCCTGCGTGCTCTGCTGCCCAGAGCCTGTAGCTCACTGTTGTCCTCAGTCCGCTGCCCAGAAC TACGGCGCTTCGCCTCGGGTGCTAACTTTCAGTACATCATCACAGAAAAGAAAGGAAAGAATAGCAGCGT GGGGCTGATCCAGTTGAACCGCCCCAAAGCACTCAATGCACTTTGCAATGGCCTGATTGAGGAGCTCAAC CAAGCACTGGAGACCTTTGAGCAAGATCCTGCTGTGGGTGCCATTGTGCTCACTGGTGGGGATAAGGCCT TTGCAGCTGGAGCTGACATCAAGGAAATGCAGAACCGAACATTTCAGGACTGTTACTCCAGCAAGTTCCT GAGCCACTGGGACCACATCACCCGGGTCAAGAAACCGGTCATCGCAGCTGTCAATGGTTATGCTCTTGGT GGGGGTTGTGAACTTGCCATGATGTGTGATATCATCTATGCTGGCGAGAAAGCCCAGTTCGGACAGCCAG AAATCCTCCTGGGGACCATCCCACGTGCTGGAGGCACTCAGAGACTCACCCGAGCAGTCGGCAAATCGCT AGCAATGGAGATGGTCCTCACTGGTGACCGCATCTCAGCTCAGGATGCAAAGCAGGCAGGTCTTGTAAGC AAGATTTTTCCTGTTGAAAAACTGGTTGAAGAAGCCATCCAATGTGCAGAAAAAATTGCCAGCAATTCTA AAATCGTAGTAGCCATGGCGAAAGAATCTGTGAATGCAGCCTTTGAGATGACGTTAACAGAAGGAAATAA GCTGGAGAAGAGGCTTTTCTATTCCACCTTTGCCACCGATGACCGGAGAGAAGGGATGACTGCATTTGTA GAGAAAAGGAAGGCCAACTTCAAAGACCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203962 protein sequence
Red=Cloning site Green=Tags(s) MAALRALLPRACSSLLSSVRCPELRRFASGANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELN QALETFEQDPAVGAIVLTGGDKAFAAGADIKEMQNRTFQDCYSSKFLSHWDHITRVKKPVIAAVNGYALG GGCELAMMCDIIYAGEKAQFGQPEILLGTIPRAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVS KIFPVEKLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGNKLEKRLFYSTFATDDRREGMTAFV EKRKANFKDH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053119 |
| ORF Size | 873 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053119.1, NM_053119.2, NP_444349.1 |
| RefSeq Size | 1491 bp |
| RefSeq ORF | 873 bp |
| Locus ID | 93747 |
| UniProt ID | Q8BH95 |
| MW | 31.6 kDa |
| Gene Summary | Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate (By similarity). Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205240 | Echs1 (untagged) - Mouse enoyl Coenzyme A hydratase, short chain, 1, mitochondrial (Echs1), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,000.00 |
|
| MG203962 | Echs1 (tGFP-tagged) - Mouse enoyl Coenzyme A hydratase, short chain, 1, mitochondrial (Echs1) |
CNY 2,850.00 |
|
| MR203962L3 | Lenti ORF clone of Echs1 (Myc-DDK-tagged) - Mouse enoyl Coenzyme A hydratase, short chain, 1, mitochondrial (Echs1), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
| MR203962L4 | Lenti ORF clone of Echs1 (mGFP-tagged) - Mouse enoyl Coenzyme A hydratase, short chain, 1, mitochondrial (Echs1), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
