M6pr (NM_010749) Mouse Tagged ORF Clone

CAT#: MR203749

  • TrueORF®

M6pr (Myc-DDK-tagged) - Mouse mannose-6-phosphate receptor, cation dependent (M6pr)

ORF Plasmid: DDK tGFP



  "NM_010749" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 2,081.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms CD-MPR; Mpr46
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR203749 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCCCTTCTCTGGCTGCTGGAGGACTGAACTGTTATTGTTGCTACTCCTGGCTGTGGCTGTGAGAG
AATCCTGGCAGATAGAAGAAAAATCGTGTGACCTGGTAGGAGAGAAGGATAAGGAGTCAAAGAACGAGGT
GGCTCTCCTGGAGAGGTTACGGCCACTGTTTAACAAAAGTTTTGAGAGTACTGTGGGCCAGGGCTCAGAC
ACATACAGCTACATATTCAGAGTATGCCGGGAAGCTAGCAACCACTCCTCTGGAGCAGGCCTGGTCCAGA
TCAACAAAAGCAATGACAAGGAGACAGTGGTTGGGAGAATCAACGAGACTCACATCTTCAATGGAAGTAA
TTGGATCATGCTGATATATAAAGGGGGTGATGAATATGACAACCACTGTGGCAAAGAGCAGCGCCGTGCA
GTGGTGATGATCTCCTGCAATCGGCACACACTAGCGGCTAATTTTAACCCAGTGTCTGAGGAACGAGGCA
AAGTCCAGGATTGCTTCTACCTCTTTGAGATGGATAGCAGCCTGGCCTGTTCACCAGAGGTCTCACACCT
CAGTGTGGGCTCGATCTTACTTGTCATATTTGCATCATTGGTTGCTGTCTATATCATTGGGGGTTTCTTA
TACCAGCGACTGGTAGTGGGGGCCAAGGGAATGGAGCAGTTTCCTCATCTGGCCTTCTGGCAGGATCTTG
GCAACCTAGTAGCTGATGGTTGTGACTTTGTGTGCCGATCCAAACCCCGCAATGTGCCTGCAGCATATCG
TGGAGTGGGAGATGACCAGCTGGGGGAAGAGTCGGAAGAAAGGGATGATCATCTGCTACCAATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR203749 protein sequence
Red=Cloning site Green=Tags(s)

MFPFSGCWRTELLLLLLLAVAVRESWQIEEKSCDLVGEKDKESKNEVALLERLRPLFNKSFESTVGQGSD
TYSYIFRVCREASNHSSGAGLVQINKSNDKETVVGRINETHIFNGSNWIMLIYKGGDEYDNHCGKEQRRA
VVMISCNRHTLAANFNPVSEERGKVQDCFYLFEMDSSLACSPEVSHLSVGSILLVIFASLVAVYIIGGFL
YQRLVVGAKGMEQFPHLAFWQDLGNLVADGCDFVCRSKPRNVPAAYRGVGDDQLGEESEERDDHLLPM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_010749
ORF Size 837 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_010749.2, NM_010749.3, NM_010749.5, NM_010749.6, NM_010749.7, NP_034879.2
RefSeq Size 2258 bp
RefSeq ORF 837 bp
Locus ID 17113
UniProt ID P24668
MW 31.2 kDa
Gene Summary Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...