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Vamp8 (NM_016794) Mouse Tagged Lenti ORF Clone
CAT＃: MR200318L3
- LentiORF®
Lenti ORF clone of Vamp8 (Myc-DDK-tagged) - Mouse vesicle-associated membrane protein 8 (Vamp8)
"NM_016794" in other vectors (4)
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CNY 4,750.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AU041171; Edb; endobrevin |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR200318).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016794 |
| ORF Size | 306 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016794.2 |
| RefSeq Size | 786 bp |
| RefSeq ORF | 306 bp |
| Locus ID | 22320 |
| UniProt ID | O70404 |
| Gene Summary | SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (By similarity). Also required for dense-granule secretion in platelets (By similarity). Plays also a role in regulated enzyme secretion in pancreatic acinar cells (PubMed:15363411). Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity). Involved in the homotypic fusion of early and late endosomes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204264 | Vamp8 (untagged) - Mouse vesicle-associated membrane protein 8 (Vamp8), (10ug) |
CNY 1,200.00 |
|
| MG200318 | Vamp8 (tGFP-tagged) - Mouse vesicle-associated membrane protein 8 (Vamp8) |
CNY 2,850.00 |
|
| MR200318 | Vamp8 (Myc-DDK-tagged) - Mouse vesicle-associated membrane protein 8 (Vamp8) |
CNY 1,200.00 |
|
| MR200318L4 | Lenti ORF clone of Vamp8 (mGFP-tagged) - Mouse vesicle-associated membrane protein 8 (Vamp8) |
CNY 4,750.00 |
