United States
Germany
Japan
United Kingdom
China
Masp1 (NM_008555) Mouse Tagged ORF Clone
CAT＃: MG226378
- TrueORF®
Masp1 (tGFP-tagged) - Mouse mannan-binding lectin serine peptidase 1 (Masp1), (10ug)
ORF Plasmid: DDK
"NM_008555" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW048060; CCPII; Crarf; Masp1/3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226378 representing NM_008555
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGTTCCTGTCTTTCTGGCGGCTCCTCCTCTACCACGCTCTGTGCCTCGCCCTGCCGGAGGTTTCAG CCCATACCGTGGAGCTAAACGAAATGTTTGGTCAGATCCAGTCACCTGGCTATCCAGATTCCTATCCAAG TGACTCTGAGGTGACATGGAATATTACTGTCCCGGAGGGGTTTCGAATCAAGCTTTACTTCATGCACTTC AACTTGGAATCCTCCTATCTTTGTGAATACGACTATGTGAAGGTAGAAACAGAAGACCAGGTGCTGGCAA CCTTTTGTGGCAGGGAGACCACCGATACTGAGCAGACCCCCGGCCAGGAAGTGGTTCTTTCGCCTGGCAC CTTCATGTCTGTCACTTTCCGGTCAGATTTCTCCAATGAGGAACGATTCACAGGCTTCGACGCCCACTAC ATGGCTGTAGATGTGGATGAGTGCAAGGAGAGGGAAGATGAAGAGCTGTCCTGTGACCACTACTGTCACA ACTACATCGGTGGCTACTACTGCTCCTGCCGCTTTGGCTACATCCTCCACACAGACAACAGGACCTGCCG AGTGGAATGCAGCGGCAATCTCTTTACCCAGAGGACAGGCACAATCACCAGCCCCGATTACCCCAACCCT TATCCCAAGAGCTCAGAATGTTCCTATACCATTGACCTGGAGGAAGGCTTCATGGTCAGCCTGCAGTTTG AGGACATTTTTGACATTGAAGACCATCCTGAGGTGCCCTGTCCCTATGACTACATTAAGATTAAAGCTGC TTCAAAAGTATGGGGTCCCTTCTGTGGAGAGAAATCCCCAGAACCAATCAGCACCCAGACTCACAGTGTC CAGATCCTATTCCGCAGCGACAACTCAGGAGAGAACCGAGGCTGGAGGCTCTCCTACAGAGCGGCAGGAA ATGAGTGCCCAAAGCTACAGCCTCCTGTGTACGGGAAAATCGAGCCCTCGCAGGCCGTGTATTCCTTCAA AGACCAAGTGCTCGTCAGCTGTGACACAGGCTACAAAGTGCTAAAGGATAACGAGGTGATGGACACATTC CAAATTGAGTGTCTGAAGGACGGTGCATGGAGTAACAAGATCCCCACCTGTAAAATTGTAGACTGTGGAG CTCCTGCAGGGCTGAAACATGGGCTAGTAACCTTCTCCACCAGAAACAACCTCACCACATACAAATCTGA GATAAGGTACTCCTGCCAACAGCCCTATTACAAGATGCTTCACAATACCACAGGTGTATATACGTGTTCT GCTCATGGGACCTGGACGAACGAAGTGCTCAAGAGAAGCCTGCCCACCTGCCTTCCAGTGTGTGGTGTCC CCAAGTTCTCCCGGAAGCAGATCTCCAGGATCTTCAATGGCCGCCCAGCCCAGAAGGGTACCATGCCATG GATTGCCATGCTGTCACACCTGAACGGACAACCCTTCTGTGGGGGTAGCCTTTTAGGTTCCAACTGGGTT TTGACAGCTGCTCACTGCCTCCACCAGTCACTTGATCCAGAAGAACCAACCCTACACAGCTCATACTTGC TCAGCCCTTCTGACTTCAAAATTATCATGGGAAAGCACTGGAGACGGCGCTCAGACGAAGACGAGCAGCA CCTGCATGTAAAGCGCACCACGCTCCACCCACTGTACAACCCCAGCACGTTTGAGAACGACCTTGGTCTG GTGGAACTGTCAGAGAGCCCGAGGCTGAACGACTTTGTGATGCCTGTCTGTCTGCCTGAGCAGCCTTCCA CTGAAGGAACCATGGTCATCGTCAGTGGCTGGGGGAAGCAGTTCTTACAGAGGTTTCCAGAGAACCTGAT GGAGATTGAAATCCCAATTGTAAACTCTGACACCTGCCAGGAGGCCTATACCCCATTGAAGAAGAAAGTG ACCAAGGACATGATCTGTGCCGGAGAAAAGGAAGGGGGGAAAGATGCCTGTGCTGGTGACTCTGGAGGCC CTATGGTGACCAAAGATGCAGAGAGAGACCAATGGTACCTGGTGGGCGTGGTGTCCTGGGGTGAAGATTG CGGGAAGAAAGATCGCTATGGAGTCTATTCTTACATCTATCCCAACAAGGACTGGATCCAGAGGATCACT GGGGTGAGGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226378 representing NM_008555
Red=Cloning site Green=Tags(s) MRFLSFWRLLLYHALCLALPEVSAHTVELNEMFGQIQSPGYPDSYPSDSEVTWNITVPEGFRIKLYFMHF NLESSYLCEYDYVKVETEDQVLATFCGRETTDTEQTPGQEVVLSPGTFMSVTFRSDFSNEERFTGFDAHY MAVDVDECKEREDEELSCDHYCHNYIGGYYCSCRFGYILHTDNRTCRVECSGNLFTQRTGTITSPDYPNP YPKSSECSYTIDLEEGFMVSLQFEDIFDIEDHPEVPCPYDYIKIKAASKVWGPFCGEKSPEPISTQTHSV QILFRSDNSGENRGWRLSYRAAGNECPKLQPPVYGKIEPSQAVYSFKDQVLVSCDTGYKVLKDNEVMDTF QIECLKDGAWSNKIPTCKIVDCGAPAGLKHGLVTFSTRNNLTTYKSEIRYSCQQPYYKMLHNTTGVYTCS AHGTWTNEVLKRSLPTCLPVCGVPKFSRKQISRIFNGRPAQKGTMPWIAMLSHLNGQPFCGGSLLGSNWV LTAAHCLHQSLDPEEPTLHSSYLLSPSDFKIIMGKHWRRRSDEDEQHLHVKRTTLHPLYNPSTFENDLGL VELSESPRLNDFVMPVCLPEQPSTEGTMVIVSGWGKQFLQRFPENLMEIEIPIVNSDTCQEAYTPLKKKV TKDMICAGEKEGGKDACAGDSGGPMVTKDAERDQWYLVGVVSWGEDCGKKDRYGVYSYIYPNKDWIQRIT GVRN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008555 |
| ORF Size | 2112 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008555.2, NP_032581.2 |
| RefSeq Size | 2752 bp |
| RefSeq ORF | 2115 bp |
| Locus ID | 17174 |
| UniProt ID | P98064 |
| Gene Summary | Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5. Also plays a role in development.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220735 | Masp1 (untagged) - Mouse mannan-binding lectin serine peptidase 1 (Masp1), (10ug) |
CNY 9,120.00 |
|
| MR226378 | Masp1 (Myc-DDK-tagged) - Mouse mannan-binding lectin serine peptidase 1 (Masp1) |
CNY 5,160.00 |
|
| MR226378L3 | Lenti ORF clone of Masp1 (Myc-DDK-tagged) - Mouse mannan-binding lectin serine peptidase 1 (Masp1) |
CNY 8,170.00 |
|
| MR226378L4 | Lenti ORF clone of Masp1 (mGFP-tagged) - Mouse mannan-binding lectin serine peptidase 1 (Masp1) |
CNY 8,170.00 |
