Dusp6 (NM_026268) Mouse Tagged ORF Clone
CAT#: MG222688
- TrueORF®
Dusp6 (tGFP-tagged) - Mouse dual specificity phosphatase 6 (Dusp6), (10ug)
ORF Plasmid: DDK
"NM_026268" in other vectors (3)
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CNY 3,140.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1300019I03Rik; MKP-3; MKP3; PYST1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222688 representing NM_026268
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATAGATACGCTCAGACCCGTGCCTTTCGCGTCGGAAATGGCGATCTGCAAGACGGTGTCGTGGCTCA ACGAGCAGCTGGAGCTGGGCAACGAACGGCTTCTGCTGATGGACTGCCGACCACAGGAGCTGTACGAGTC GTCGCACATCGAATCTGCCATTAATGTGGCCATCCCGGGCATCATGCTGCGGCGTCTGCAGAAGGGCAAC CTGCCCGTGCGTGCGCTCTTCACGCGCTGCGAGGACCGGGACCGCTTTACCAGGCGCTGCGGCACCGACA CCGTGGTGCTGTACGACGAGAATAGCAGCGACTGGAATGAGAACACTGGTGGAGAGTCGGTCCTCGGGCT GCTGCTCAAGAAACTCAAAGACGAGGGCTGCCGGGCGTTCTACCTGGAAGGTGGCTTCAGTAAGTTCCAG GCCGAGTTCGCCCTGCACTGCGAGACCAATCTAGACGGCTCGTGCAGCAGCAGTTCCCCGCCTTTGCCAG TGCTGGGGCTCGGGGGCCTGCGGATCAGCTCCGACTCTTCCTCGGACATTGAGTCTGACCTTGACCGAGA CCCCAATAGTGCAACGGACTCTGATGGCAGCCCGCTGTCCAACAGCCAGCCTTCCTTCCCGGTGGAGATT TTGCCCTTCCTTTACCTGGGCTGTGCCAAGGACTCGACCAACTTGGACGTGTTGGAAGAGTTTGGCATCA AGTACATCTTGAATGTCACCCCCAATTTGCCCAATCTGTTTGAGAATGCGGGCGAGTTCAAATACAAGCA AATTCCTATCTCGGATCACTGGAGCCAAAACCTGTCCCAGTTTTTCCCTGAGGCCATTTCTTTCATAGAT GAAGCCCGAGGCAAAAACTGTGGTGTCCTGGTGCATTGCTTGGCAGGTATCAGCCGCTCTGTCACCGTGA CAGTGGCGTACCTCATGCAGAAGCTCAACCTGTCCATGAACGATGCTTACGACATTGTTAAGATGAAGAA GTCCAACATCTCCCCCAACTTCAACTTCATGGGCCAGCTGCTTGACTTCGAAAGGACCCTGGGACTCAGC AGCCCTTGTGACAACCGCGTCCCCACTCCGCAGCTGTACTTCACCACGCCCTCCAACCAGAACGTCTACC AGGTGGACTCCCTGCAGTCTACG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222688 representing NM_026268
Red=Cloning site Green=Tags(s) MIDTLRPVPFASEMAICKTVSWLNEQLELGNERLLLMDCRPQELYESSHIESAINVAIPGIMLRRLQKGN LPVRALFTRCEDRDRFTRRCGTDTVVLYDENSSDWNENTGGESVLGLLLKKLKDEGCRAFYLEGGFSKFQ AEFALHCETNLDGSCSSSSPPLPVLGLGGLRISSDSSSDIESDLDRDPNSATDSDGSPLSNSQPSFPVEI LPFLYLGCAKDSTNLDVLEEFGIKYILNVTPNLPNLFENAGEFKYKQIPISDHWSQNLSQFFPEAISFID EARGKNCGVLVHCLAGISRSVTVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLS SPCDNRVPTPQLYFTTPSNQNVYQVDSLQST TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_026268 |
ORF Size | 1143 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_026268.3, NP_080544.1 |
RefSeq Size | 2814 bp |
RefSeq ORF | 1146 bp |
Locus ID | 67603 |
UniProt ID | Q9DBB1 |
Gene Summary | Inactivates MAP kinases. Has a specificity for the ERK family (By similarity). Plays an important role in alleviating acute postoperative pain (PubMed:24155322, PubMed:28405172). Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (PubMed:24155322). Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain (PubMed:28405172).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Dynamic ROS Control by TIGAR Regulates the Initiation and Progression of Pancreatic Cancer
,null,
Cancer Cell
,PubMed ID 31983610
[Dusp6]
|
Documents
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FAQs |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR222688 | Dusp6 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 6 (Dusp6) |
CNY 2,850.00 |
|
MR222688L3 | Lenti ORF clone of Dusp6 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 6 (Dusp6) |
CNY 4,750.00 |
|
MR222688L4 | Lenti ORF clone of Dusp6 (mGFP-tagged) - Mouse dual specificity phosphatase 6 (Dusp6) |
CNY 4,750.00 |