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Rad54l (NM_009015) Mouse Tagged ORF Clone
CAT＃: MG221645
- TrueORF®
Rad54l (tGFP-tagged) - Mouse RAD54 like (S. cerevisiae) (Rad54l) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_009015" in other vectors (5)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RAD54 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221645 representing NM_009015
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGGAGCTTAGCTCCCAGCCAGTTGGCCAGGAGGAAACCAGAAGACAGATCATCAGATGATGAAG ACTGGCAGCCTGGGACAGTAACTCCTAAGAAACGCAAGTCCAGCAGTGAGACCCAGGTCCAGGAGTGTTT CCTGTCTCCTTTTCGGAAGCCCTTGACTCAGCTACTCAACCGGCCACCTTGTCTGGATAGCAGTCAACAT GAAGCATTTATTCGAAGTATTTTGTCAAAGCCTTTCAAGGTCCCCATCCCAAATTATCAAGGTCCCCTGG GCTCTCGTGCATTGGGCCTGAAAAGGGTTGGAGTTCGTCGTGCCCTTCATGACCCTCTGGAAGAAGGTGC CTTGGTTCTCTATGAGCCTCCCCCACTCAGCGCCCATGACCAACTGAAGCTTGACAAGGAAAAACTCCCT GTTCATGTGGTTGTTGATCCTATTCTCAGTAAGGTATTGCGGCCTCATCAGAGAGAGGGAGTGAAGTTCC TATGGGAGTGTGTCACCAGTCGTCGAATTCCTGGAAGCCATGGCTGCATCATGGCTGATGAGATGGGCCT GGGGAAGACACTGCAGTGCATCACATTGATGTGGACACTTTTACGCCAGAGCCCAGAATGCAAGCCAGAA ATCGAGAAGGCAGTGGTGGTGTCACCTTCTAGCCTGGTGAAGAACTGGTACAATGAGGTTGAGAAATGGC TTGGAGGGAGGATTCAACCTCTGGCCATCGATGGAGGCTCGAAGGACGAGATAGACCGAAAACTGGAAGG ATTCATGAACCAGCGTGGAGCTAGAGTGCCTTCCCCCATCCTCATCATTTCCTATGAGACTTTCCGCCTG CATGTTGGAGTTCTTAAAAAAGGAAATGTTGGACTGGTCATATGTGACGAGGGCCACAGGCTAAAGAACT CTGAGAATCAGACTTACCAGGCTCTGGACAGCTTGAATACCAGCCGGCGGGTGCTAATCTCCGGGACCCC CATCCAAAATGATTTGCTGGAGTATTTCAGCTTGGTGCACTTTGTAAATTCTGGCATTTTGGGAACTGCC CATGAATTCAAGAAGCATTTTGAGTTGCCAATTTTGAAGAGTCGAGATGCAGCTGCCAGTGAGGCAGACA GGCAGCGTGGGGAGGAGCGTCTGCGGGAGCTCATCGGTATTGTGAACAGGTGCCTGATACGGAGAACATC TGATATCCTCTCTAAGTATCTGCCCGTGAAGATTGAGCAGGTGGTTTGTTGTAGGCTGACACCCCTTCAA ACTGAGCTATACAAGAGATTTCTGAGACAGGCTAAGCCTGAAGAAGAATTGCGTGAGGGCAAGATGAGTG TGTCTTCCCTGTCTTCTATCACCTCTCTAAAGAAGCTGTGTAATCATCCAGCTCTAATCTATGACAAGTG TGTGGCAGAGGAGGATGGCTTTGAGGGCACTTTGGGTATCTTCCCACCTGGTTATAACTCTAAAGCTGTA GAGCCACAGTTGTCAGGTAAGATGCTGGTCCTTGATTACATTCTGGCCGTGACTCGAAGCCGTAGCAGTG ACAAAGTCGTGCTGGTGTCTAATTATACTCAGACATTGGATCTCTTTGAAAAGCTGTGCCGGGTTCGAAG GTACTTGTATGTTCGCCTGGATGGCACGATGTCCATTAAGAAGCGAGCCAAGGTTGTGGAGCGCTTCAAT AGCCCATCGAGCCCTGATTTTGTTTTCATGCTGAGCAGCAAAGCTGGGGGCTGTGGTCTTAACCTCATTG GTGCTAACCGACTGGTCATGTTTGATCCTGACTGGAATCCAGCCAATGATGAACAAGCTATGGCCCGAGT CTGGCGTGATGGTCAAAAGAAGATCTGCTATATCTACCGACTGCTATCTGCAGGAACAATTGAGGAGAAG ATCTTTCAGCGGCAGAGCCACAAGAAGGCTCTGAGCAGCTGTGTGGTGGACGAGGAGCAGGATGTGGAGC GCCACTTTTCTCTTGGTGAGCTTAAAGAGCTGTTTACTCTGGATGAAGCAAGCCTCAGTGACACACATGA CAGATTGCATTGCCGCCGTTGTGTAAACAACCGTCAGGTCTGGCCACCCCCTGATGGTTCTGACTGCACT TCAGACCTGGCTCAGTGGAACCACAGCACAGATAAACGAGGGCTCCAGGATGAGGTACTCCAGGCTGCCT GGGATGCTTCATCTACAGCCATCACCTTCGTCTTCCACCAGCGTTCTCATGAGGAGCAGCGCGGTCTTCA C ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221645 representing NM_009015
Red=Cloning site Green=Tags(s) MRRSLAPSQLARRKPEDRSSDDEDWQPGTVTPKKRKSSSETQVQECFLSPFRKPLTQLLNRPPCLDSSQH EAFIRSILSKPFKVPIPNYQGPLGSRALGLKRVGVRRALHDPLEEGALVLYEPPPLSAHDQLKLDKEKLP VHVVVDPILSKVLRPHQREGVKFLWECVTSRRIPGSHGCIMADEMGLGKTLQCITLMWTLLRQSPECKPE IEKAVVVSPSSLVKNWYNEVEKWLGGRIQPLAIDGGSKDEIDRKLEGFMNQRGARVPSPILIISYETFRL HVGVLKKGNVGLVICDEGHRLKNSENQTYQALDSLNTSRRVLISGTPIQNDLLEYFSLVHFVNSGILGTA HEFKKHFELPILKSRDAAASEADRQRGEERLRELIGIVNRCLIRRTSDILSKYLPVKIEQVVCCRLTPLQ TELYKRFLRQAKPEEELREGKMSVSSLSSITSLKKLCNHPALIYDKCVAEEDGFEGTLGIFPPGYNSKAV EPQLSGKMLVLDYILAVTRSRSSDKVVLVSNYTQTLDLFEKLCRVRRYLYVRLDGTMSIKKRAKVVERFN SPSSPDFVFMLSSKAGGCGLNLIGANRLVMFDPDWNPANDEQAMARVWRDGQKKICYIYRLLSAGTIEEK IFQRQSHKKALSSCVVDEEQDVERHFSLGELKELFTLDEASLSDTHDRLHCRRCVNNRQVWPPPDGSDCT SDLAQWNHSTDKRGLQDEVLQAAWDASSTAITFVFHQRSHEEQRGLH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009015 |
| ORF Size | 2241 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009015.2 |
| RefSeq Size | 3093 bp |
| RefSeq ORF | 2244 bp |
| Locus ID | 19366 |
| UniProt ID | P70270 |
| Gene Summary | Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). Deficient mice also show significantly shorter telomeres than wild-type controls, indicating that the protein activity plays an essential role in telomere length maintenance in mammals. Deficiency also resulted in an increased frequency of end-to-end chromosome fusions involving telomeres compared to the controls, suggesting a putative role in telomere capping. Non-homologous end joining (NHEJ) and homologous recombination (HR) represent the two major pathways of DNA double-strand break (DSB) repair in eukaryotic cells. LIG4 and RAD54L cooperate to support cellular proliferation, repair spontaneous DSBs, and prevent chromosome and single chromatid aberrations.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205394 | Rad54l (untagged) - Mouse RAD54 like (S. cerevisiae) (Rad54l), transcript variant 1, (10ug) |
CNY 2,000.00 |
|
| MG210443 | Rad54l (tGFP-tagged) - Mouse RAD54 like (S. cerevisiae) (Rad54l) |
CNY 6,370.00 |
|
| MR221645 | Rad54l (Myc-DDK-tagged) - Mouse RAD54 like (S. cerevisiae) (Rad54l), transcript variant 1 |
CNY 5,480.00 |
|
| MR221645L3 | Lenti ORF clone of Rad54l (Myc-DDK-tagged) - Mouse RAD54 like (S. cerevisiae) (Rad54l), transcript variant 1 |
CNY 7,700.00 |
|
| MR221645L4 | Lenti ORF clone of Rad54l (mGFP-tagged) - Mouse RAD54 like (S. cerevisiae) (Rad54l), transcript variant 1 |
CNY 7,700.00 |
