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Trim45 (NM_001165953) Mouse Tagged ORF Clone
CAT＃: MG217723
- TrueORF®
Trim45 (tGFP-tagged) - Mouse tripartite motif-containing 45 (Trim45) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001165953" in other vectors (4)
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CNY 8,270.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4921530N01Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217723 representing NM_001165953
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGAAATCAGGAAGCCGCTTCTGGGTTTCGTGCACAAACTCCAAGATGCGAACGCGTCTGGAAGCT CAGGCAAGACTCATTGTCCTACATGTCTGAGGCTTTTCAAAGTCCCCAGGCTCTTGCCTTGCTTGCACAC AGTGTGCACCACGTGTCTGGAAAAGCTGGATCCATTCTCAGTAGTGGACATCCGTGGGGGTGACTCAGAT ACAAGCTCTGAGGGGTCAGTATTCCAGGACCCCGAGTTATGTAGCCTGCAGCCACAGATCGGCATCCTCT GTCCGGTATGTGATGCTCAAGTGGACCTGCCCTTGGGTGGAGTGAAGGCTTTAACGGTAGACCACCTGGC CATGAATGATGTGCTGCTGGAGAACCTGCGTGGGGAAGGCCAGGGCCTGGTGTGTGACCTGTGCAGCGAC AGAGAAGTAGAGAAGAGGTGTCAGACCTGCAAAGCCAATCTCTGCCACTTCTGCTGCCAGGCTCATAGGC GACAGAAGAAGACGACATATCACACCATGGTGGACCTAAAAGACCTGAAAGGCTACAGCCAGGTTGGAAA GCCCATCCTATGTCCTTCTCACCCTGCAGAGGAGCTGAGGCTGTTCTGTGAACTCTGTGACCGGCCGGTG TGTCGGGACTGTGTAGTTGGGGAGCACCGAGAGCACCCCTATGACTTCACCAGCAATGTCATCCACAAGC ATGGAGATTCTGTGCGGGAGCTGCTTCGAGACACCCAGCCCCATGTGGAAGCCCTGGAAGATGCCCTGGC ACAGATTAAAAGTGTGAACAACGCCCTCCAAGAGCGAGTGGAGGCAGTGGCAGCTGACGTCCGGACATTC TCTGAGGGCTACATCAAAGCCATCGAGGAACACCGGGACAAGCTGCTGCAGCAGCTGGATGACATACGGA TCCAGAGGGAAACCGCTTTACAGCTGCAGAAAGCGCAGCTGGAACAGCTGCTGGCAGATATGCGGACTGG AGTGGAGTTCACCGAGCATCTGCTGACCAGCGGCTCGGACTTAGAGATTCTCATCACCAAGGGAGTGGTT GTAGAGCGGCTCAGGAAGCTGAATAAAGTTGAATACAGTGCCCGCCCAGGAGTGAACCATAAGATCTGCT TCTCCCCCCAGGAGAAGGCAGGCCAGTGCCAAGGCTATGAAGTGTATGGGGCCATTAACACGCAGGAAGT TGATCCAGCTCAGTGTGTCCTTCAGGGAGAAGATCTCCACAGAGCTCGAGAGAAACAGACAGCCTCTTTT ACCTTGTTCTGTAAGGATGCCTCGGGACAGAGCATGGGCAGGGGAGGAGATAATGTCCATGTGGAAGTTG TCCCCAAAGATAAGAAGGACAGTCCGATCAGAACAGTGGTCCAGGACAACAAGGATGGGTCGTACCGCGT TTCCTACACCCCCAAGGAGCCTGGCATCTACACCGTGTGGGTCTGCATCAGAGAGCAGCACGTGCAGGGC TCACCATTCAACGTGACCGTGAGGAGGAAGCATCGGCCACATCCGGGTGTGTTTCACTGCTGCACCTTCT GCTCCAGTGGAGGACAGAAAGCTGCCCGCTGTGCCTGCGGAGGCACCATGCCGGCCCGCAGGCCCAAGCA GCAGCAAGCAAGGGTCCGTCCCTTTGCTGCCTCACAGGTGGATACCTTGGCTGTGGCCACGGACACAAAG GCCACCCGGGCCGTCCACACTGGTCTTGCTGTGGGAAGTTCATTGAGAAGTCCGAGTGCTCGTTCACGAG TGGGCAGGGCGCCCCGAGGAGTCTGCTTAGGACCGTGGCGCTCTGATGCTTCCTGGGGACAAGTCGGGCC AGGTAACCCTGAAGCTAGCATAACTTCAGCTATGCAGAGAACCCAGAGCTTGAGTAGTTCCAAAGAGACT GACAGAATTAAAGACGAAGTGCTTTCTCTAACATCAGACTTCAAGTGCTTTCTGTGTGGGCATCTCTCGT GTACCATGGCGGAGTGCAGGCCTCCCCTACACCGCTCCTCAAGGCAGGCTGCTGATCTACTCCACGGAAA GCTCTCCTGTTTTATACGGGCATTGAGGTGTCTGTGCCATGGGACCATGAGCTCCTGGAGCAGGGAGGAC TTTATGCCTTCCTTTCCACTGTGTTGCAGCCTCTCCGGTTTACCCCGAGCTAGAGTTTCAGGGCTTCTCC TGGAGGATGAAACCCCTCTGGCCCATCATTTGTTTGTTGTATACTTTCAGTGGTTTCCTAGTACACAGTT TATGATGATTATCACGGTTTTGCCGTCTAGA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217723 representing NM_001165953
Red=Cloning site Green=Tags(s) MSEIRKPLLGFVHKLQDANASGSSGKTHCPTCLRLFKVPRLLPCLHTVCTTCLEKLDPFSVVDIRGGDSD TSSEGSVFQDPELCSLQPQIGILCPVCDAQVDLPLGGVKALTVDHLAMNDVLLENLRGEGQGLVCDLCSD REVEKRCQTCKANLCHFCCQAHRRQKKTTYHTMVDLKDLKGYSQVGKPILCPSHPAEELRLFCELCDRPV CRDCVVGEHREHPYDFTSNVIHKHGDSVRELLRDTQPHVEALEDALAQIKSVNNALQERVEAVAADVRTF SEGYIKAIEEHRDKLLQQLDDIRIQRETALQLQKAQLEQLLADMRTGVEFTEHLLTSGSDLEILITKGVV VERLRKLNKVEYSARPGVNHKICFSPQEKAGQCQGYEVYGAINTQEVDPAQCVLQGEDLHRAREKQTASF TLFCKDASGQSMGRGGDNVHVEVVPKDKKDSPIRTVVQDNKDGSYRVSYTPKEPGIYTVWVCIREQHVQG SPFNVTVRRKHRPHPGVFHCCTFCSSGGQKAARCACGGTMPARRPKQQQARVRPFAASQVDTLAVATDTK ATRAVHTGLAVGSSLRSPSARSRVGRAPRGVCLGPWRSDASWGQVGPGNPEASITSAMQRTQSLSSSKET DRIKDEVLSLTSDFKCFLCGHLSCTMAECRPPLHRSSRQAADLLHGKLSCFIRALRCLCHGTMSSWSRED FMPSFPLCCSLSGLPRARVSGLLLEDETPLAHHLFVVYFQWFPSTQFMMIITVLPSR SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001165953 |
| ORF Size | 2271 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001165953.1, NP_001159425.1 |
| RefSeq Size | 4668 bp |
| RefSeq ORF | 2274 bp |
| Locus ID | 229644 |
| UniProt ID | Q6PFY8 |
| Gene Summary | May act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221398 | Trim45 (untagged) - Mouse tripartite motif-containing 45 (Trim45), transcript variant 1, (10ug) |
CNY 9,790.00 |
|
| MR217723 | Trim45 (Myc-DDK-tagged) - Mouse tripartite motif-containing 45 (Trim45), transcript variant 1 |
CNY 8,312.00 |
|
| MR217723L3 | Lenti ORF clone of Trim45 (Myc-DDK-tagged) - Mouse tripartite motif-containing 45 (Trim45), transcript variant 1 |
CNY 9,410.00 |
|
| MR217723L4 | Lenti ORF clone of Trim45 (mGFP-tagged) - Mouse tripartite motif-containing 45 (Trim45), transcript variant 1 |
CNY 9,410.00 |
