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Uba1 (NM_009457) Mouse Tagged ORF Clone
CAT＃: MG211556
- TrueORF®
Uba1 (tGFP-tagged) - Mouse ubiquitin-activating enzyme E1, Chr X (Ube1x)
"NM_009457" in other vectors (5)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | A1; A1S9; Sb; Sbx; Ube-1; Ube1x |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211556 representing NM_009457
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGCTCGCCGCTGTCCAAGAAACGTCGCGTGTCCGGGCCTGATCCAAAGCCGGGTTCTAACTGCT CCCCTGCACAGTCTGCGCTGTCCGAAGTGTCCTCAGTGCCAACCAACGGAATGGCGAAGAACGGCAGTGA AGCAGACATAGACGAGAGCCTTTACTCCCGGCAGCTGTACGTTTTGGGCCATGAGGCAATGAAAATGCTC CAGACATCCAGCGTCCTTGTCTCAGGCTTGCGGGGCTTGGGTGTAGAAATTGCTAAGAACATCATCCTTG GTGGGGTCAAGGCTGTCACCCTACATGACCAAGGAACTACCCAGTGGGCTGATCTCTCCTCCCAGTTTTA CCTTCGGGAGGAGGACATTGGTAAAAATCGAGCGGAGGTATCCCAGCCCCGACTTGCTGAACTCAACAGC TATGTACCTGTCACTGCCTACACTGGGCCTCTTGTCGAGGACTTCCTTAGTAGCTTCCAGGTGGTGGTCC TCACCAACAGCCCCCTGGAAGCCCAGCTGCGAGTGGGGGAGTTCTGTCATAGCCGTGGTATCAAGCTAGT GGTGGCAGATACAAGAGGCCTGTTTGGGCAACTTTTCTGTGATTTTGGAGAGGAAATGGTCCTCACAGAT TCCAATGGGGAGCAGCCACTCAGTGCTATGGTTTCAATGGTCACCAAGGACAACCCCGGTGTGGTTACCT GCCTTGATGAGGCCCGACATGGCTTTGAGACTGGTGACTTCGTCTCATTCTCAGAAGTACAGGGCATGAT CCAACTCAATGGATGTCAGCCCATGGAGATCAAAGTGCTGGGTCCTTATACCTTTAGTATCTGTGACACT TCCAACTTCTCTGACTACATCCGTGGAGGCATCGTCAGTCAGGTCAAAGTACCGAAAAAGATTAGTTTTA AATCCTTGCCAGCATCACTGGTAGAGCCTGACTTTGTGATGACTGACTTTGCCAAGTATTCTCGCCCTGC CCAACTGCACATTGGCTTCCAAGCTCTGCACCAATTCTGTGCTCTGCACAACCAACCACCTCGACCACGA AATGAGGAAGATGCAACAGAGCTGGTGGGCCTGGCTCAGGCTGTAAACGCTCGGTCCCCACCTTCAGTAA AACAGAACAGCTTGGATGAAGACCTTATTCGGAAGCTAGCTTATGTTGCTGCTGGGGACCTGGCACCCAT AAATGCTTTCATTGGGGGCCTTGCTGCCCAGGAAGTCATGAAGGCCTGCTCTGGAAAGTTTATGCCCATC ATGCAGTGGTTGTACTTTGATGCTCTTGAATGTCTCCCAGAGGACAAAGAGGCTCTGACAGAGGAGAAGT GCCTCCCACGTCAGAACCGTTACGATGGGCAGGTAGCTGTATTTGGGTCAGACTTTCAGGAGAAGCTGAG CAAGCAAAAGTACTTCCTGGTGGGTGCAGGGGCCATTGGCTGTGAATTGCTCAAGAACTTTGCCATGATT GGGCTGGGTTGTGGAGAGGGTGGAGAGGTCGTGGTCACAGACATGGACACCATTGAGAAATCAAATCTGA ACCGACAGTTTCTCTTCCGGCCCTGGGATGTCACGAAGTTAAAGTCTGACACGGCCGCTGCAGCTGTGCG CCAGATGAATCCTTACATCCAGGTGACAAGCCACCAGAACCGTGTAGGTCCTGACACTGAGCGCATCTAT GATGATGATTTCTTCCAAAATTTGGATGGTGTGGCCAATGCTCTGGACAACATAGATGCCCGCATGTACA TGGATCGCCGATGTGTGTACTATCGGAAGCCACTGCTAGAGTCTGGCACACTGGGCACAAAGGGCAACGT GCAGGTGGTAATCCCCTTCCTGACAGAATCCTACAGCTCTAGCCAGGATCCACCAGAGAAATCCATCCCC ATTTGTACCCTGAAAAACTTTCCCAATGCCATCGAACACACTCTTCAGTGGGCCCGGGATGAATTTGAAG GCCTTTTCAAGCAGCCAGCAGAAAATGTTAATCAGTACCTCACAGACTCCAAATTTGTGGAGCGGACATT GCGGCTGGCTGGTACCCAGCCATTGGAGGTGCTGGAGGCTGTGCAGCGCAGCCTGGTGTTGCAGCGACCA CAGACTTGGGGAGACTGTGTGACCTGGGCCTGCCACCACTGGCACACCCAGTACTGTAACAACATCCGGC AACTGCTGCACAACTTTCCTCCTGACCAGCTCACCAGCTCAGGGGCCCCTTTCTGGTCTGGACCCAAACG CTGTCCACACCCACTTACTTTTGATGTTAACAATACATTGCATCTGGATTATGTGATGGCTGCTGCCAAC CTTTTTGCCCAGACCTATGGGTTGACTGGATCCCAAGACCGAGCTGCTGTGGCCTCACTCCTGCAGTCAG TACAAGTCCCAGAGTTCACCCCCAAGTCTGGTGTCAAGATTCATGTTTCTGACCAGGAGTTGCAGAGTGC CAATGCCTCTGTTGATGACAGCCGTCTTGAGGAGCTCAAAGCCACATTGCCCAGCCCAGACAAGTTACCC GGATTTAAGATGTACCCCATTGATTTTGAGAAGGATGATGACAGCAATTTCCACATGGATTTCATTGTGG CTGCATCCAATCTTCGGGCCGAAAACTATGATATTTCCCCTGCAGACCGACACAAGAGCAAGCTGATTGC AGGGAAGATCATCCCAGCCATTGCCACAACCACAGCTGCTGTGGTTGGCCTTGTGTGTCTGGAGCTCTAC AAGGTAGTTCAAGGGCACCAACAGCTCGATTCCTACAAAAATGGTTTCCTGAACTTGGCCCTGCCCTTCT TTGGGTTTTCTGAACCTCTTGCTGCACCTCGTCACCAGTACTATAATCAAGAGTGGACATTGTGGGATCG CTTTGAAGTACAAGGGCTGCAGCCTAATGGTGAGGAGATGACCCTCAAGCAGTTCCTTGATTACTTTAAG ACAGAGCACAAATTGGAGATCACCATGCTGTCCCAGGGCGTGTCCATGCTCTATTCTTTCTTCATGCCAG CTGCTAAGCTCAAGGAACGATTGGATCAGCCGATGACAGAGATTGTGAGCCGAGTGTCAAAGAGAAAGCT GGGCCGCCATGTGCGGGCACTGGTGCTTGAGCTGTGCTGCAACGATGAAAGCGGCGAGGACGTCGAGGTC CCTTATGTCCGATATACCATTCGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211556 representing NM_009457
Red=Cloning site Green=Tags(s) MSSSPLSKKRRVSGPDPKPGSNCSPAQSALSEVSSVPTNGMAKNGSEADIDESLYSRQLYVLGHEAMKML QTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTTQWADLSSQFYLREEDIGKNRAEVSQPRLAELNS YVPVTAYTGPLVEDFLSSFQVVVLTNSPLEAQLRVGEFCHSRGIKLVVADTRGLFGQLFCDFGEEMVLTD SNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFETGDFVSFSEVQGMIQLNGCQPMEIKVLGPYTFSICDT SNFSDYIRGGIVSQVKVPKKISFKSLPASLVEPDFVMTDFAKYSRPAQLHIGFQALHQFCALHNQPPRPR NEEDATELVGLAQAVNARSPPSVKQNSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPI MQWLYFDALECLPEDKEALTEEKCLPRQNRYDGQVAVFGSDFQEKLSKQKYFLVGAGAIGCELLKNFAMI GLGCGEGGEVVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPYIQVTSHQNRVGPDTERIY DDDFFQNLDGVANALDNIDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIP ICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDSKFVERTLRLAGTQPLEVLEAVQRSLVLQRP QTWGDCVTWACHHWHTQYCNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNTLHLDYVMAAAN LFAQTYGLTGSQDRAAVASLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLP GFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDISPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELY KVVQGHQQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFK TEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEV PYVRYTIR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009457 |
| ORF Size | 4065 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009457.3, NP_033483.1 |
| RefSeq Size | 3950 bp |
| RefSeq ORF | 3357 bp |
| Locus ID | 22201 |
| UniProt ID | Q02053 |
| Gene Summary | This gene encodes a member of the ubiquitin-activating E1 family. The encoded protein initiates the ubiquitin activation and transfer cascade, catalyzing the first step in ubiquitin conjugation to mark cellular proteins for proteasome degradation. Ubiquitin activating enzymes use ATP to form a thioester between a conserved catalytic cysteine of the enzyme and the C-terminal carboxylate of ubiquitin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223393 | Uba1 (untagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 1, (10ug) |
CNY 13,590.00 |
|
| MG219278 | Uba1 (tGFP-tagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1) transcript variant 1, (10ug) |
CNY 11,590.00 |
|
| MR219278 | Uba1 (Myc-DDK-tagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 1 |
CNY 7,376.00 |
|
| MR219278L3 | Lenti ORF clone of Uba1 (Myc-DDK-tagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 1 |
CNY 12,450.00 |
|
| MR219278L4 | Lenti ORF clone of Uba1 (mGFP-tagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 1 |
CNY 12,450.00 |
