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Dgkb (NM_178681) Mouse Tagged ORF Clone
CAT＃: MG210702
- TrueORF®
Dgkb (tGFP-tagged) - Mouse diacylglycerol kinase, beta (Dgkb)
ORF Plasmid: DDK
"NM_178681" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 90kda; 6430574F24; C630029D13Rik; DAGK2; DGK; DGK-beta; mKIAA0718 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210702 representing NM_178681
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAAACCAGGAAAAATGGGCCCATCTCAGCCCCTCAGAATTTTCCCAACTTCAGAAATATGCTGAGT ATTCTACAAAGAAATTAAAGGATGTTCTTGAAGAATTCCATGGTAATGGCGTGCTTGCAAAGTATAATCC TGAAGGGACCATAGATTTTGAAGGCTTCAAGCTATTCATGAAGACCTTTCTGGAAGCTGAGCTGCCTGAC GATTTCACCGCCCACCTTTTTATGTCATTCAGCAACAAATTTCCCCATTCTAGCCCAAATGTAAAGAGTA AGCCCGCTCTTCTTTCAGGTGGTCTCAGGATGAATAAGGGTGCCATCACCCCTCCACGTTCCTCTCCAGC AAATACATGCTCCCCAGAAGTGATCCACCTCAAGGATATCGTCTGCTACCTGTCTCTTCTCGAAAGAGGA AGACCCGAGGATAAGCTCGAGTTTATGTTTCGCCTTTATGACACCGATGGGAATGGCTTCCTGGACAGCT CGGAATTAGAAAATATTATTGGTCAGATGATGCACGTGGCAGAGTACCTCGAATGGGATGTTACCGAACT TAATCCGATCCTTCATGAAATGATGGAAGAAATTGACTATGACCGGGATGGCACTGTGTCTCTGGAGGAG TGGATTCAAGGAGGAATGACAACTATCCCACTTCTAGTCCTGCTGGGCTTGGAGAATAATGTGAAGGATG ATGGACAGCATGTGTGGCGACTCAAGCACTTTAACAAGCCTGCCTACTGCAATCTCTGCCTGAACATGCT GATTGGCGTGGGGAAGCAGGGCCTCTGCTGCTCCTTCTGCAAGTACACGGTCCATGAGCGCTGTGTGGCC AGAGCCCCGCCCTCCTGCATCAAGACATATGTGAAGTCCAAGAAAAACACAGACGTCATGCACCACTATT GGGTCGAAGGCAATTGCCCAACCAAGTGCGATAAGTGCCACAAAACAGTGAAATGTTACCAGGGCCTGAC GGGCCTGCATTGTGTTTGGTGCCAGACCACACTGCACAATAAATGTGCTTCTCATCTGAAGCCCGAGTGT GACTGTGGACCCTTGAAGGACCATATTTTGCCACCTACCACAATCTGTCCAGTTGTATTGACTATGCCCA GTGCTGGAGCCTCAGTTCCTGAGGAAAGACAGTCCACAGCTAAAAAGGAAAAGAGCAGCTCCCAGCAGCC AAATAAAGCAACAGACAAAAATAAAATGCAAAGAGCTAACTCAGTTACTATGGATGGACAAGGTTTGCAG ATCACTCCTGTTCCTGGCACACATCCGCTTCTAGTTTTTGTCAACCCTAAAAGTGGTGGGAAGCAAGGAG AACGAATTTACAGAAAATTCCAGTATCTCCTAAATCCTCGTCAGGTTTACAGTCTTTCTGGAAATGGACC AATGCCAGGGTTACACTTTTTTCGCGATGTCCCTGACTTCAGAGTGTTAGCATGCGGTGGAGATGGGACT GTGGGCTGGATTTTGGATTGCATAGAGAAGGCCAATGTAGTCAAGCACCCTCCAGTTGCCATCCTGCCTC TTGGGACTGGCAATGACTTAGCAAGGTGCCTGCGGTGGGGTGGAGGTTATGAAGGGGAAAATCTTATGAA GATCCTAAAAGACATTGAAAGCAGCACAGAAATCATGCTGGACAGGTGGAAGTTTGAAGTCACACCCAAC GACAAAGATGAGAAAGGAGACCCTGTGCCTTACAGCATCATCAATAACTACTTTTCCATTGGCGTGGATG CCTCCATTGCACACAGATTCCACATCATGAGAGAAAAGCACCCAGAGAAATTCAACAGCCGAATGAAGAA CAAATTTTGGTACTTTGAGTTTGGCACATCTGAAACCTTCTCAGCGACCTGCAAGAAGCTGCATGAATCT GTAGAAATAGAATGTGATGGAGTACAAATAGATTTGATAAACATCTCTCTGGAAGGAATTGCAATTTTGA ATATACCAAGCATGCATGGAGGATCTAATCTTTGGGGAGAGTCTAAAAAGAAAAGAAGCCATCGACGAAT AGAGAAAAAAGGCTCTGACAAAAGACCCACTCTCACAGATGCAAAAGAGCTGAAGTTTGCAAGTCAAGAT CTGAGTGACCAGCTGCTGGAGGTCGTTGGCTTGGAAGGAGCAATGGAAATGGGCCAAATTTACACGGGCC TGAAAAGTGCGGGCCGGCGGCTGGCTCAGTGCTCCTCTGTGGTTATCAGAACAAGCAAGTCACTGCCAAT GCAGATTGATGGAGAGCCCTGGATGCAGACCCCATGCACAATAAAAATTACACACAAGAACCAAGCCCCA ATGCTGATGGGCCCTCCACCCAAAACTGGATTATTCTGCTCACTTATCAAAAGAACAAGAAACCGAAGCA AGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210702 representing NM_178681
Red=Cloning site Green=Tags(s) MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAKYNPEGTIDFEGFKLFMKTFLEAELPD DFTAHLFMSFSNKFPHSSPNVKSKPALLSGGLRMNKGAITPPRSSPANTCSPEVIHLKDIVCYLSLLERG RPEDKLEFMFRLYDTDGNGFLDSSELENIIGQMMHVAEYLEWDVTELNPILHEMMEEIDYDRDGTVSLEE WIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVA RAPPSCIKTYVKSKKNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQTTLHNKCASHLKPEC DCGPLKDHILPPTTICPVVLTMPSAGASVPEERQSTAKKEKSSSQQPNKATDKNKMQRANSVTMDGQGLQ ITPVPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGNGPMPGLHFFRDVPDFRVLACGGDGT VGWILDCIEKANVVKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIESSTEIMLDRWKFEVTPN DKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHES VEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQD LSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAP MLMGPPPKTGLFCSLIKRTRNRSKE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178681 |
| ORF Size | 2385 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178681.5 |
| RefSeq Size | 5545 bp |
| RefSeq ORF | 2388 bp |
| Locus ID | 217480 |
| UniProt ID | Q6NS52 |
| Gene Summary | Exhibits high phosphorylation activity for long-chain diacylglycerols.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203527 | Dgkb (untagged) - Mouse diacylglycerol kinase, beta (Dgkb), (10ug) |
CNY 2,000.00 |
|
| MR210702 | Dgkb (Myc-DDK-tagged) - Mouse diacylglycerol kinase, beta (Dgkb) |
CNY 5,800.00 |
|
| MR210702L3 | Lenti ORF clone of Dgkb (Myc-DDK-tagged) - Mouse diacylglycerol kinase, beta (Dgkb) |
CNY 7,700.00 |
|
| MR210702L4 | Lenti ORF clone of Dgkb (mGFP-tagged) - Mouse diacylglycerol kinase, beta (Dgkb) |
CNY 7,700.00 |
